Ethanolic Extract of Papaya (Carica papaya) Leaf Exhibits Estrogenic Effects In Vivo and In Silico
The menopause women have the low level of estrogen in the body. The lack of estrogen changes physiological function in women’s body that affects in health condition. Carica papaya L. leaf contains flavonoid quercetin which exhibits estrogenic effect. The aim of this study is to determine the estroge...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Cancer Chemoprevention
2012-06-01
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| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/60 |
| _version_ | 1829105272797265920 |
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| author | Raisatun Nisa Sugiyanto Rahmi Khamsita Marvin Lambertus Rohmad Yudi Utomo Ratna Asmah Susidarti |
| author_facet | Raisatun Nisa Sugiyanto Rahmi Khamsita Marvin Lambertus Rohmad Yudi Utomo Ratna Asmah Susidarti |
| author_sort | Raisatun Nisa Sugiyanto |
| collection | DOAJ |
| description | The menopause women have the low level of estrogen in the body. The lack of estrogen changes physiological function in women’s body that affects in health condition. Carica papaya L. leaf contains flavonoid quercetin which exhibits estrogenic effect. The aim of this study is to determine the estrogenic effect of papaya leaves extract (PLE) in vivo, and in silico. Papaya leaves were extracted by ethanol 70% maceration. The in silico study were done by molecular docking between quersetin and Estrogen Receptor (ERα and ERβ) to obtain the docking score. Based on this study, docking score of quercetin was almost similar to the native ligand of ER. The in vivo study was done as follow: 36 female rats Sprague Dawley divided into six groups. The groups are shame-ovariectomized (S-OVX), control ovariectomized (OVX), CMC-Na control (OVX+CMC-Na), positive control (OVX+Estradiol), and the PLE treatment groups dose 750 mg/kgBW (OVX+750mg/kgBW) and dose 1000 mg/kgBW (OVX+1000 mg/kgBW). Administrations of PLE were done in three weeks orally, while estradiol was administrated intraperitonially. The mammae and uterine were sliced for analysis. Based on the study, the treatment of PLE increased the number of mammae lobules and uterine weight as well as estrogen does. In summary, PLE can be developed as a source of phytoestrogens.
Keywords: Carica papaya L., phytoestrogen, estrogen receptor, mammae lobule, uterine |
| first_indexed | 2024-12-12T06:02:22Z |
| format | Article |
| id | doaj.art-d80bb0e15bae4a7b947ddde32de39d08 |
| institution | Directory Open Access Journal |
| issn | 2088-0197 2355-8989 |
| language | English |
| last_indexed | 2024-12-12T06:02:22Z |
| publishDate | 2012-06-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj.art-d80bb0e15bae4a7b947ddde32de39d082022-12-22T00:35:22ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892012-06-013238439010.14499/indonesianjcanchemoprev3iss2pp384-39061Ethanolic Extract of Papaya (Carica papaya) Leaf Exhibits Estrogenic Effects In Vivo and In SilicoRaisatun Nisa Sugiyanto0Rahmi Khamsita1Marvin Lambertus2Rohmad Yudi Utomo3Ratna Asmah Susidarti4Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaDepartement of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah MadaThe menopause women have the low level of estrogen in the body. The lack of estrogen changes physiological function in women’s body that affects in health condition. Carica papaya L. leaf contains flavonoid quercetin which exhibits estrogenic effect. The aim of this study is to determine the estrogenic effect of papaya leaves extract (PLE) in vivo, and in silico. Papaya leaves were extracted by ethanol 70% maceration. The in silico study were done by molecular docking between quersetin and Estrogen Receptor (ERα and ERβ) to obtain the docking score. Based on this study, docking score of quercetin was almost similar to the native ligand of ER. The in vivo study was done as follow: 36 female rats Sprague Dawley divided into six groups. The groups are shame-ovariectomized (S-OVX), control ovariectomized (OVX), CMC-Na control (OVX+CMC-Na), positive control (OVX+Estradiol), and the PLE treatment groups dose 750 mg/kgBW (OVX+750mg/kgBW) and dose 1000 mg/kgBW (OVX+1000 mg/kgBW). Administrations of PLE were done in three weeks orally, while estradiol was administrated intraperitonially. The mammae and uterine were sliced for analysis. Based on the study, the treatment of PLE increased the number of mammae lobules and uterine weight as well as estrogen does. In summary, PLE can be developed as a source of phytoestrogens. Keywords: Carica papaya L., phytoestrogen, estrogen receptor, mammae lobule, uterinehttps://ijcc.chemoprev.org/index.php/ijcc/article/view/60 |
| spellingShingle | Raisatun Nisa Sugiyanto Rahmi Khamsita Marvin Lambertus Rohmad Yudi Utomo Ratna Asmah Susidarti Ethanolic Extract of Papaya (Carica papaya) Leaf Exhibits Estrogenic Effects In Vivo and In Silico ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | Ethanolic Extract of Papaya (Carica papaya) Leaf Exhibits Estrogenic Effects In Vivo and In Silico |
| title_full | Ethanolic Extract of Papaya (Carica papaya) Leaf Exhibits Estrogenic Effects In Vivo and In Silico |
| title_fullStr | Ethanolic Extract of Papaya (Carica papaya) Leaf Exhibits Estrogenic Effects In Vivo and In Silico |
| title_full_unstemmed | Ethanolic Extract of Papaya (Carica papaya) Leaf Exhibits Estrogenic Effects In Vivo and In Silico |
| title_short | Ethanolic Extract of Papaya (Carica papaya) Leaf Exhibits Estrogenic Effects In Vivo and In Silico |
| title_sort | ethanolic extract of papaya carica papaya leaf exhibits estrogenic effects in vivo and in silico |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/60 |
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