Green synthesis of silver nanoparticles at low temperature in a fast pace with unique DPPH radical scavenging and selective cytotoxicity against MCF-7 and BT-20 tumor cell lines
''Black Peel Pomegranate'' is a rare pomegranate cultivar that its specific features are still uncovered particularly in the bio-nano researches. The present study was organized to evaluate this pomegranate's potential in the biosynthesis of silver nanoparticles as well as b...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-12-01
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| Series: | Biotechnology Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X19305788 |
| _version_ | 1818507331934093312 |
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| author | Sadegh Khorrami Atefeh Zarepour Ali Zarrabi |
| author_facet | Sadegh Khorrami Atefeh Zarepour Ali Zarrabi |
| author_sort | Sadegh Khorrami |
| collection | DOAJ |
| description | ''Black Peel Pomegranate'' is a rare pomegranate cultivar that its specific features are still uncovered particularly in the bio-nano researches. The present study was organized to evaluate this pomegranate's potential in the biosynthesis of silver nanoparticles as well as bio-medical activities. According to the results, the pomegranate peel extract incredibly inhibited 100 % of DPPH free radicals (EC50 = 5 μg/mL). This extract also induced more than 70 % cell death in the treated breast tumor cell lines, BT-20 and MCF-7. Interestingly, the extract was capable of biosynthesis very stable and small (15.6 nm) silver nanoparticles at ambient temperature in an ultra-fast pace. Likewise, these nanoparticles inhibited 77 % of DPPH free radicals (EC50 = 9 μg/mL). Although this antioxidant capacity was lower than that of the extract, instead, the anticancer activity of the synthesized nanoparticles was significantly enhanced, so that they led to more than 81 % and 89 % cell death in the breast tumor cell lines BT-20 and MCF-7, respectively. Considerably, neither the extract nor the biosynthesized silver nanoparticles, showed significant toxicity against non-tumor cell lines (L-929) at the same concentrations. These features of the biosynthesized nanoparticles were quite outstanding in comparison with chemical/commercial ones. Overall, the present study introduces black peel pomegranate as a worthy bio-agent in the biosynthesis of silver nanoparticles with unique activities as well as a cancer treatment. Keywords: Black peel pomegranate, Green synthesis, Selective cytotoxicity, Antioxidant agents, Anticancer agents |
| first_indexed | 2024-12-10T22:17:01Z |
| format | Article |
| id | doaj.art-d811551f7b90476f85d4923b7212710c |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-10T22:17:01Z |
| publishDate | 2019-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-d811551f7b90476f85d4923b7212710c2022-12-22T01:31:26ZengElsevierBiotechnology Reports2215-017X2019-12-0124Green synthesis of silver nanoparticles at low temperature in a fast pace with unique DPPH radical scavenging and selective cytotoxicity against MCF-7 and BT-20 tumor cell linesSadegh Khorrami0Atefeh Zarepour1Ali Zarrabi2Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran; Corresponding authors.Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, IranSabanci University, Nanotechnology Research, and Application Center (SUNUM), Istanbul, 34956, Turkey; Corresponding authors.''Black Peel Pomegranate'' is a rare pomegranate cultivar that its specific features are still uncovered particularly in the bio-nano researches. The present study was organized to evaluate this pomegranate's potential in the biosynthesis of silver nanoparticles as well as bio-medical activities. According to the results, the pomegranate peel extract incredibly inhibited 100 % of DPPH free radicals (EC50 = 5 μg/mL). This extract also induced more than 70 % cell death in the treated breast tumor cell lines, BT-20 and MCF-7. Interestingly, the extract was capable of biosynthesis very stable and small (15.6 nm) silver nanoparticles at ambient temperature in an ultra-fast pace. Likewise, these nanoparticles inhibited 77 % of DPPH free radicals (EC50 = 9 μg/mL). Although this antioxidant capacity was lower than that of the extract, instead, the anticancer activity of the synthesized nanoparticles was significantly enhanced, so that they led to more than 81 % and 89 % cell death in the breast tumor cell lines BT-20 and MCF-7, respectively. Considerably, neither the extract nor the biosynthesized silver nanoparticles, showed significant toxicity against non-tumor cell lines (L-929) at the same concentrations. These features of the biosynthesized nanoparticles were quite outstanding in comparison with chemical/commercial ones. Overall, the present study introduces black peel pomegranate as a worthy bio-agent in the biosynthesis of silver nanoparticles with unique activities as well as a cancer treatment. Keywords: Black peel pomegranate, Green synthesis, Selective cytotoxicity, Antioxidant agents, Anticancer agentshttp://www.sciencedirect.com/science/article/pii/S2215017X19305788 |
| spellingShingle | Sadegh Khorrami Atefeh Zarepour Ali Zarrabi Green synthesis of silver nanoparticles at low temperature in a fast pace with unique DPPH radical scavenging and selective cytotoxicity against MCF-7 and BT-20 tumor cell lines Biotechnology Reports |
| title | Green synthesis of silver nanoparticles at low temperature in a fast pace with unique DPPH radical scavenging and selective cytotoxicity against MCF-7 and BT-20 tumor cell lines |
| title_full | Green synthesis of silver nanoparticles at low temperature in a fast pace with unique DPPH radical scavenging and selective cytotoxicity against MCF-7 and BT-20 tumor cell lines |
| title_fullStr | Green synthesis of silver nanoparticles at low temperature in a fast pace with unique DPPH radical scavenging and selective cytotoxicity against MCF-7 and BT-20 tumor cell lines |
| title_full_unstemmed | Green synthesis of silver nanoparticles at low temperature in a fast pace with unique DPPH radical scavenging and selective cytotoxicity against MCF-7 and BT-20 tumor cell lines |
| title_short | Green synthesis of silver nanoparticles at low temperature in a fast pace with unique DPPH radical scavenging and selective cytotoxicity against MCF-7 and BT-20 tumor cell lines |
| title_sort | green synthesis of silver nanoparticles at low temperature in a fast pace with unique dpph radical scavenging and selective cytotoxicity against mcf 7 and bt 20 tumor cell lines |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X19305788 |
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