Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF Treatment
Background: ZEB1 plays a role in epithelial-to-mesenchymal transition and acts as a repressor of E-cadherin, TGF-β, and Wnt/β-catenin. Since ZEB1 protein is expressed in estrogen-responsive tissues, and expression of the gene in the normal ovary and endometrium is positively correlated with high est...
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MDPI AG
2023-08-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/12/17/5652 |
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| author | Ioannis Chrysanthopoulos Despoina Mavrogianni Eirini Drakaki Anastasios Potiris Athanasios Zikopoulos Athanasios Zachariou Ekaterini Domali Peter Drakakis Sofoklis Stavros |
| author_facet | Ioannis Chrysanthopoulos Despoina Mavrogianni Eirini Drakaki Anastasios Potiris Athanasios Zikopoulos Athanasios Zachariou Ekaterini Domali Peter Drakakis Sofoklis Stavros |
| author_sort | Ioannis Chrysanthopoulos |
| collection | DOAJ |
| description | Background: ZEB1 plays a role in epithelial-to-mesenchymal transition and acts as a repressor of E-cadherin, TGF-β, and Wnt/β-catenin. Since ZEB1 protein is expressed in estrogen-responsive tissues, and expression of the gene in the normal ovary and endometrium is positively correlated with high estrogen levels, we performed a direct analysis of granulosa cell samples to determine whether there are any significant changes in zeb1 expression during folliculogenesis. Methods: ZEB1 expression levels were measured in the granulosa cells of 56 infertile women undergoing IVF treatment. RNA extraction from granulosa cells was performed along with reverse transcription quantitative polymerase chain reaction (RT-qPCR) with SYBR Green I to determine zeb1 gene expression levels. Statistical analysis was performed by using <i>t</i>-test, while possible correlations of the expression of ZEB1 protein with body mass index (BMI), age, number of oocytes, and oocyte maturation were investigated. Results: Zeb1 gene expression levels correlate significantly with body mass index (BMI) and age, but not with oocyte number and oocyte maturation stage. Obese women demonstrate a higher expression level of zeb1 gene compared to normal and overweight women. Moreover, zeb1 gene is overexpressed in women aged 35–40 years old and is under-expressed in women >40 years old. Conclusions: ZEB1 expression should be further investigated as it may unveil new potential findings of the zeb1 gene’s role in female fertility and its use as a biomarker in fertility workups. |
| first_indexed | 2024-03-10T23:19:54Z |
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| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T23:19:54Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-d812ea003d9f4c5cac281187324cdeb32023-11-19T08:23:34ZengMDPI AGJournal of Clinical Medicine2077-03832023-08-011217565210.3390/jcm12175652Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF TreatmentIoannis Chrysanthopoulos0Despoina Mavrogianni1Eirini Drakaki2Anastasios Potiris3Athanasios Zikopoulos4Athanasios Zachariou5Ekaterini Domali6Peter Drakakis7Sofoklis Stavros8First Department of Obstetrics and Gynecology, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 115 28 Athens, GreeceFirst Department of Obstetrics and Gynecology, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 115 28 Athens, GreeceFirst Department of Obstetrics and Gynecology, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 115 28 Athens, GreeceThird Department of Obstetrics and Gynecology, University General Hospital “ATTIKON”, Medical School, National and Kapodistrian University of Athens, 12462 Athens, GreeceDepartment of Obstetrics and Gynecology, Royal Cornwall Hospital, Treliske, Truro TR1 3LQ, UKLaboratory of Spermatology, Department of Urology, School of Health Sciences, University of Ioannina, 451 10 Ioannina, GreeceFirst Department of Obstetrics and Gynecology, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 115 28 Athens, GreeceFirst Department of Obstetrics and Gynecology, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 115 28 Athens, GreeceThird Department of Obstetrics and Gynecology, University General Hospital “ATTIKON”, Medical School, National and Kapodistrian University of Athens, 12462 Athens, GreeceBackground: ZEB1 plays a role in epithelial-to-mesenchymal transition and acts as a repressor of E-cadherin, TGF-β, and Wnt/β-catenin. Since ZEB1 protein is expressed in estrogen-responsive tissues, and expression of the gene in the normal ovary and endometrium is positively correlated with high estrogen levels, we performed a direct analysis of granulosa cell samples to determine whether there are any significant changes in zeb1 expression during folliculogenesis. Methods: ZEB1 expression levels were measured in the granulosa cells of 56 infertile women undergoing IVF treatment. RNA extraction from granulosa cells was performed along with reverse transcription quantitative polymerase chain reaction (RT-qPCR) with SYBR Green I to determine zeb1 gene expression levels. Statistical analysis was performed by using <i>t</i>-test, while possible correlations of the expression of ZEB1 protein with body mass index (BMI), age, number of oocytes, and oocyte maturation were investigated. Results: Zeb1 gene expression levels correlate significantly with body mass index (BMI) and age, but not with oocyte number and oocyte maturation stage. Obese women demonstrate a higher expression level of zeb1 gene compared to normal and overweight women. Moreover, zeb1 gene is overexpressed in women aged 35–40 years old and is under-expressed in women >40 years old. Conclusions: ZEB1 expression should be further investigated as it may unveil new potential findings of the zeb1 gene’s role in female fertility and its use as a biomarker in fertility workups.https://www.mdpi.com/2077-0383/12/17/5652infertilityin vitro fertilization (IVF)ZEB1 genegranulosa cellsbody mass index (BMI)age |
| spellingShingle | Ioannis Chrysanthopoulos Despoina Mavrogianni Eirini Drakaki Anastasios Potiris Athanasios Zikopoulos Athanasios Zachariou Ekaterini Domali Peter Drakakis Sofoklis Stavros Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF Treatment Journal of Clinical Medicine infertility in vitro fertilization (IVF) ZEB1 gene granulosa cells body mass index (BMI) age |
| title | Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF Treatment |
| title_full | Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF Treatment |
| title_fullStr | Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF Treatment |
| title_full_unstemmed | Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF Treatment |
| title_short | Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF Treatment |
| title_sort | detection of zeb1 gene in granulosa cells in women undergoing ivf treatment |
| topic | infertility in vitro fertilization (IVF) ZEB1 gene granulosa cells body mass index (BMI) age |
| url | https://www.mdpi.com/2077-0383/12/17/5652 |
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