Enhancement of Virus Infection Using Dynamic Cell Culture in a Microchannel
With increasing interest in induced pluripotent stem cells (iPSCs) in the field of stem cell research, highly efficient infection of somatic cells with virus factors is gaining importance. This paper presents a method of employing microfluidic devices for dynamic cell culture and virus infection in...
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| Format: | Article |
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MDPI AG
2018-09-01
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| Series: | Micromachines |
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| Online Access: | http://www.mdpi.com/2072-666X/9/10/482 |
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| author | Jeong A Kim Hye Jin Choi Chul Min Kim Hee Kyung Jin Jae-sung Bae Gyu Man Kim |
| author_facet | Jeong A Kim Hye Jin Choi Chul Min Kim Hee Kyung Jin Jae-sung Bae Gyu Man Kim |
| author_sort | Jeong A Kim |
| collection | DOAJ |
| description | With increasing interest in induced pluripotent stem cells (iPSCs) in the field of stem cell research, highly efficient infection of somatic cells with virus factors is gaining importance. This paper presents a method of employing microfluidic devices for dynamic cell culture and virus infection in a microchannel. The closed space in the microchannel provided a better environment for viruses to diffuse and contact cell surfaces to infect cells. The microfluidic devices were fabricated by photolithography and soft lithography. NIH/3T3 fibroblast cells were cultured in the microfluidic device in static and dynamic conditions and compared with the conventional culture method of using Petri dishes. Virus infection was evaluated using an enhanced green fluorescent protein virus as a model. Dynamic culture in the microchannel showed similar growth of cells to that in Petri dish culture, but the virus infection efficiency was four-times higher. The proposed dynamic culture system could be useful in iPSC research by providing efficient virus infection tools. |
| first_indexed | 2024-12-11T18:10:16Z |
| format | Article |
| id | doaj.art-d81509d8ec87422a9cc02938173da326 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-11T18:10:16Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-d81509d8ec87422a9cc02938173da3262022-12-22T00:55:36ZengMDPI AGMicromachines2072-666X2018-09-0191048210.3390/mi9100482mi9100482Enhancement of Virus Infection Using Dynamic Cell Culture in a MicrochannelJeong A Kim0Hye Jin Choi1Chul Min Kim2Hee Kyung Jin3Jae-sung Bae4Gyu Man Kim5School of Mechanical Engineering, Kyungpook National University, 80 Daehakro, Buk-gu, Daegu 41566, KoreaSchool of Mechanical Engineering, Kyungpook National University, 80 Daehakro, Buk-gu, Daegu 41566, KoreaSchool of Mechanical Engineering, Kyungpook National University, 80 Daehakro, Buk-gu, Daegu 41566, KoreaDepartment of Laboratory Animal Medicine, College of Veterinary Medicine, Kyungpook National University, 80 Daehakro, Buk-gu, Daegu 41566, KoreaDepartment of Physiology, School of Medicine, Kyungpook National University, 680 Gukchaebosang-ro, Jung-Gu, Daegu 41944, KoreaSchool of Mechanical Engineering, Kyungpook National University, 80 Daehakro, Buk-gu, Daegu 41566, KoreaWith increasing interest in induced pluripotent stem cells (iPSCs) in the field of stem cell research, highly efficient infection of somatic cells with virus factors is gaining importance. This paper presents a method of employing microfluidic devices for dynamic cell culture and virus infection in a microchannel. The closed space in the microchannel provided a better environment for viruses to diffuse and contact cell surfaces to infect cells. The microfluidic devices were fabricated by photolithography and soft lithography. NIH/3T3 fibroblast cells were cultured in the microfluidic device in static and dynamic conditions and compared with the conventional culture method of using Petri dishes. Virus infection was evaluated using an enhanced green fluorescent protein virus as a model. Dynamic culture in the microchannel showed similar growth of cells to that in Petri dish culture, but the virus infection efficiency was four-times higher. The proposed dynamic culture system could be useful in iPSC research by providing efficient virus infection tools.http://www.mdpi.com/2072-666X/9/10/482virus infectionmicrochanneldynamic cell cultureinduced pluripotent stem cells (iPSCs) |
| spellingShingle | Jeong A Kim Hye Jin Choi Chul Min Kim Hee Kyung Jin Jae-sung Bae Gyu Man Kim Enhancement of Virus Infection Using Dynamic Cell Culture in a Microchannel Micromachines virus infection microchannel dynamic cell culture induced pluripotent stem cells (iPSCs) |
| title | Enhancement of Virus Infection Using Dynamic Cell Culture in a Microchannel |
| title_full | Enhancement of Virus Infection Using Dynamic Cell Culture in a Microchannel |
| title_fullStr | Enhancement of Virus Infection Using Dynamic Cell Culture in a Microchannel |
| title_full_unstemmed | Enhancement of Virus Infection Using Dynamic Cell Culture in a Microchannel |
| title_short | Enhancement of Virus Infection Using Dynamic Cell Culture in a Microchannel |
| title_sort | enhancement of virus infection using dynamic cell culture in a microchannel |
| topic | virus infection microchannel dynamic cell culture induced pluripotent stem cells (iPSCs) |
| url | http://www.mdpi.com/2072-666X/9/10/482 |
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