Evaluation of infection course in mice induced by L. major in presence of positively charged liposomes containing CpG ODN
Abstract An inoculation of virulent Leishmania major is known as leishmanization (LZ) which is proven to be the most effective control measure against Cutaneous Leishmaniasis (CL). However, using LZ is restricted due to various side effects such as uncontrolled lesion development. In the present res...
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Mashhad University of Medical Sciences
2013-01-01
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| Series: | Nanomedicine Journal |
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| Online Access: | http://nmj.mums.ac.ir/?_action=showPDF&article=700&_ob=da350093d42b1f32a1d63facd7a27302&fileName=full_text.pdf |
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| author | Hesamoddin Hoseinjani Mahmoud Reza Jaafari Ali Khamesipour Azam Abbasi Zahra Saberi Ali Badiee |
| author_facet | Hesamoddin Hoseinjani Mahmoud Reza Jaafari Ali Khamesipour Azam Abbasi Zahra Saberi Ali Badiee |
| author_sort | Hesamoddin Hoseinjani |
| collection | DOAJ |
| description | Abstract An inoculation of virulent Leishmania major is known as leishmanization (LZ) which is proven to be the most effective control measure against Cutaneous Leishmaniasis (CL). However, using LZ is restricted due to various side effects such as uncontrolled lesion development. In the present research, the efficacy of cationic nanoliposomes containing CpG oligodeoxynucleotides (CpG ODN) as an improved adjuvant delivery system was studied to diminish the lesion development and infection course of L. major after inoculation into the mice. BALB/c mice were inoculated subcutaneously (SC) with L. major plus empty DSPC, DSPC (CpG ODN), DSPC (Non CpG ODN), empty DMPC, DMPC (CpG ODN), DMPC (Non CpG ODN) or HEPES buffer. The results showed that group of mice received DMPC (CpG ODN) nanoliposomes developed a significantly smaller lesion and showed minimum number of L. major in the spleen and draining lymph nodes. In addition, using DMPC (CpG ODN) liposomes resulted in a Th1 type of immune response with a preponderance of IgG2a isotype which is concurrent with the production of DMPC (CpG) induced IFN-γ in the spleen of the mice. Taken together, the results suggested that immune modulation using DMPC (CpG ODN) nanoliposomes might be a practical approach to improve the safety of LZ |
| first_indexed | 2024-12-24T04:12:26Z |
| format | Article |
| id | doaj.art-d815a0d20cc64c4d84ad03e37f300d60 |
| institution | Directory Open Access Journal |
| issn | 2322-3049 2322-5904 |
| language | English |
| last_indexed | 2024-12-24T04:12:26Z |
| publishDate | 2013-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Nanomedicine Journal |
| spelling | doaj.art-d815a0d20cc64c4d84ad03e37f300d602022-12-21T17:16:02ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042013-01-01112837700Evaluation of infection course in mice induced by L. major in presence of positively charged liposomes containing CpG ODNHesamoddin Hoseinjani0Mahmoud Reza Jaafari1Ali Khamesipour2Azam Abbasi3Zahra Saberi4Ali Badiee5Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Science, Mashhad, IranNanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Science, Mashhad, Iran Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Science, Mashhad, IranCenter for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, IranNanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Science, Mashhad, Iran1Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Science, Mashhad, IranNanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Science, Mashhad, IranAbstract An inoculation of virulent Leishmania major is known as leishmanization (LZ) which is proven to be the most effective control measure against Cutaneous Leishmaniasis (CL). However, using LZ is restricted due to various side effects such as uncontrolled lesion development. In the present research, the efficacy of cationic nanoliposomes containing CpG oligodeoxynucleotides (CpG ODN) as an improved adjuvant delivery system was studied to diminish the lesion development and infection course of L. major after inoculation into the mice. BALB/c mice were inoculated subcutaneously (SC) with L. major plus empty DSPC, DSPC (CpG ODN), DSPC (Non CpG ODN), empty DMPC, DMPC (CpG ODN), DMPC (Non CpG ODN) or HEPES buffer. The results showed that group of mice received DMPC (CpG ODN) nanoliposomes developed a significantly smaller lesion and showed minimum number of L. major in the spleen and draining lymph nodes. In addition, using DMPC (CpG ODN) liposomes resulted in a Th1 type of immune response with a preponderance of IgG2a isotype which is concurrent with the production of DMPC (CpG) induced IFN-γ in the spleen of the mice. Taken together, the results suggested that immune modulation using DMPC (CpG ODN) nanoliposomes might be a practical approach to improve the safety of LZhttp://nmj.mums.ac.ir/?_action=showPDF&article=700&_ob=da350093d42b1f32a1d63facd7a27302&fileName=full_text.pdfDMPC (CpG ODN) nanoliposomesCpG ODNL. majorLeishmanizationImmune response |
| spellingShingle | Hesamoddin Hoseinjani Mahmoud Reza Jaafari Ali Khamesipour Azam Abbasi Zahra Saberi Ali Badiee Evaluation of infection course in mice induced by L. major in presence of positively charged liposomes containing CpG ODN Nanomedicine Journal DMPC (CpG ODN) nanoliposomes CpG ODN L. major Leishmanization Immune response |
| title | Evaluation of infection course in mice induced by L. major in presence of positively charged liposomes containing CpG ODN |
| title_full | Evaluation of infection course in mice induced by L. major in presence of positively charged liposomes containing CpG ODN |
| title_fullStr | Evaluation of infection course in mice induced by L. major in presence of positively charged liposomes containing CpG ODN |
| title_full_unstemmed | Evaluation of infection course in mice induced by L. major in presence of positively charged liposomes containing CpG ODN |
| title_short | Evaluation of infection course in mice induced by L. major in presence of positively charged liposomes containing CpG ODN |
| title_sort | evaluation of infection course in mice induced by l major in presence of positively charged liposomes containing cpg odn |
| topic | DMPC (CpG ODN) nanoliposomes CpG ODN L. major Leishmanization Immune response |
| url | http://nmj.mums.ac.ir/?_action=showPDF&article=700&_ob=da350093d42b1f32a1d63facd7a27302&fileName=full_text.pdf |
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