Multifunctional effects of Lactobacillus sakei HEM 224 on the gastrointestinal tract and airway inflammation
Abstract Mucosal tissues serve as the first defense line and their commensal microbiota play a role in sustaining of host health. This study aimed to isolate and evaluate a putative probiotic strain on various mucosal regions. Lactobacillus sakei HEM 224 was isolated from traditional Korean kimchi a...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-45043-0 |
| _version_ | 1827156769763229696 |
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| author | Hye-Shin Kim Hanna Oh Bobae Kim Yosep Ji Wilhelm H. Holzapfel Hyeji Kang Karina Arellano-Ayala |
| author_facet | Hye-Shin Kim Hanna Oh Bobae Kim Yosep Ji Wilhelm H. Holzapfel Hyeji Kang Karina Arellano-Ayala |
| author_sort | Hye-Shin Kim |
| collection | DOAJ |
| description | Abstract Mucosal tissues serve as the first defense line and their commensal microbiota play a role in sustaining of host health. This study aimed to isolate and evaluate a putative probiotic strain on various mucosal regions. Lactobacillus sakei HEM 224 was isolated from traditional Korean kimchi and identified. In the safety assessment L. sakei HEM 224 showed negative results for hemolysis, biogenic amine production and transferable antibiotic resistance. The probiotic potential of strain HEM 224 in diverse mucosal areas was shown in two different models, viz. a murine model with colitis induced by dextran sulfate sodium (DSS) and an allergic airway inflammation model induced by ovalbumin (OVA). In the colitis model, oral administration of L. sakei HEM 224 improved colitis physiology with immunomodulation, enhancing barrier components and gut microbiota alteration. In the allergic airway inflammation model, the intranasal administration of the strain decreased type 2 inflammation and enhanced epithelial barrier integrity from the airways. These results demonstrate that L. sakei HEM 224 can ameliorate inflammatory conditions in both the gastrointestinal and respiratory tracts through the reinforcement of the epithelial barrier and immunomodulation. |
| first_indexed | 2024-03-10T22:00:50Z |
| format | Article |
| id | doaj.art-d817432b4f3e4aecbeecb337d8314698 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2025-03-20T23:14:43Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d817432b4f3e4aecbeecb337d83146982024-08-04T11:24:00ZengNature PortfolioScientific Reports2045-23222023-10-0113111210.1038/s41598-023-45043-0Multifunctional effects of Lactobacillus sakei HEM 224 on the gastrointestinal tract and airway inflammationHye-Shin Kim0Hanna Oh1Bobae Kim2Yosep Ji3Wilhelm H. Holzapfel4Hyeji Kang5Karina Arellano-Ayala6Department of Advanced Convergence, Handong Global UniversityDepartment of Advanced Convergence, Handong Global UniversityHEM Pharma Inc.HEM Pharma Inc.Department of Advanced Convergence, Handong Global UniversityHEM Pharma Inc.Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC)Abstract Mucosal tissues serve as the first defense line and their commensal microbiota play a role in sustaining of host health. This study aimed to isolate and evaluate a putative probiotic strain on various mucosal regions. Lactobacillus sakei HEM 224 was isolated from traditional Korean kimchi and identified. In the safety assessment L. sakei HEM 224 showed negative results for hemolysis, biogenic amine production and transferable antibiotic resistance. The probiotic potential of strain HEM 224 in diverse mucosal areas was shown in two different models, viz. a murine model with colitis induced by dextran sulfate sodium (DSS) and an allergic airway inflammation model induced by ovalbumin (OVA). In the colitis model, oral administration of L. sakei HEM 224 improved colitis physiology with immunomodulation, enhancing barrier components and gut microbiota alteration. In the allergic airway inflammation model, the intranasal administration of the strain decreased type 2 inflammation and enhanced epithelial barrier integrity from the airways. These results demonstrate that L. sakei HEM 224 can ameliorate inflammatory conditions in both the gastrointestinal and respiratory tracts through the reinforcement of the epithelial barrier and immunomodulation.https://doi.org/10.1038/s41598-023-45043-0 |
| spellingShingle | Hye-Shin Kim Hanna Oh Bobae Kim Yosep Ji Wilhelm H. Holzapfel Hyeji Kang Karina Arellano-Ayala Multifunctional effects of Lactobacillus sakei HEM 224 on the gastrointestinal tract and airway inflammation Scientific Reports |
| title | Multifunctional effects of Lactobacillus sakei HEM 224 on the gastrointestinal tract and airway inflammation |
| title_full | Multifunctional effects of Lactobacillus sakei HEM 224 on the gastrointestinal tract and airway inflammation |
| title_fullStr | Multifunctional effects of Lactobacillus sakei HEM 224 on the gastrointestinal tract and airway inflammation |
| title_full_unstemmed | Multifunctional effects of Lactobacillus sakei HEM 224 on the gastrointestinal tract and airway inflammation |
| title_short | Multifunctional effects of Lactobacillus sakei HEM 224 on the gastrointestinal tract and airway inflammation |
| title_sort | multifunctional effects of lactobacillus sakei hem 224 on the gastrointestinal tract and airway inflammation |
| url | https://doi.org/10.1038/s41598-023-45043-0 |
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