Exploring Piezo1, Piezo2, and TMEM150C in human brain tissues and their correlation with brain biomechanical characteristics
Abstract Unraveling the intricate relationship between mechanical factors and brain activity is a pivotal endeavor, yet the underlying mechanistic model of signaling pathways in brain mechanotransduction remains enigmatic. To bridge this gap, we introduced an in situ multi-scale platform, through wh...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | Molecular Brain |
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| Online Access: | https://doi.org/10.1186/s13041-023-01071-5 |
| _version_ | 1827399299059679232 |
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| author | Arjun Raha Yuning Wu Lily Zhong Jatheeshan Raveenthiran Minji Hong Aftab Taiyab Li Wang Bill Wang Fei Geng |
| author_facet | Arjun Raha Yuning Wu Lily Zhong Jatheeshan Raveenthiran Minji Hong Aftab Taiyab Li Wang Bill Wang Fei Geng |
| author_sort | Arjun Raha |
| collection | DOAJ |
| description | Abstract Unraveling the intricate relationship between mechanical factors and brain activity is a pivotal endeavor, yet the underlying mechanistic model of signaling pathways in brain mechanotransduction remains enigmatic. To bridge this gap, we introduced an in situ multi-scale platform, through which we delineate comprehensive brain biomechanical traits in white matter (WM), grey-white matter junctions (GW junction), and the pons across human brain tissue from four distinct donors. We investigate the three-dimensional expression patterns of Piezo1, Piezo2, and TMEM150C, while also examining their associated histological features and mechanotransduction signaling networks, particularly focusing on the YAP/β-catenin axis. Our results showed that the biomechanical characteristics (including stiffness, spring term, and equilibrium stress) associated with Piezo1 vary depending on the specific region. Moving beyond Piezo1, our result demonstrated the significant positive correlations between Piezo2 expression and stiffness in the WM. Meanwhile, the expression of Piezo2 and TMEM150C was shown to be correlated to viscoelastic properties in the pons and WM. Given the heterogeneity of brain tissue, we investigated the three-dimensional expression of Piezo1, Piezo2, and TMEM150C. Our results suggested that three mechanosensitive proteins remained consistent across different vertical planes within the tissue sections. Our findings not only establish Piezo1, Piezo2, and TMEM150C as pivotal mechanosensors that regulate the region-specific mechanotransduction activities but also unveil the paradigm connecting brain mechanical properties and mechanotransduction activities and the variations between individuals. |
| first_indexed | 2024-03-08T19:43:53Z |
| format | Article |
| id | doaj.art-d81c29dd47a246029d06b180e51db1a9 |
| institution | Directory Open Access Journal |
| issn | 1756-6606 |
| language | English |
| last_indexed | 2024-03-08T19:43:53Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj.art-d81c29dd47a246029d06b180e51db1a92023-12-24T12:32:25ZengBMCMolecular Brain1756-66062023-12-0116111110.1186/s13041-023-01071-5Exploring Piezo1, Piezo2, and TMEM150C in human brain tissues and their correlation with brain biomechanical characteristicsArjun Raha0Yuning Wu1Lily Zhong2Jatheeshan Raveenthiran3Minji Hong4Aftab Taiyab5Li Wang6Bill Wang7Fei Geng8School of Biomedical Engineering, McMaster UniversitySchool of Biomedical Engineering, McMaster UniversityIntegrated Biomedical Engineering and Health Sciences Program, McMaster UniversityIntegrated Biomedical Engineering and Health Sciences Program, McMaster UniversityIntegrated Biomedical Engineering and Health Sciences Program, McMaster UniversityDepartment of Pathology and Molecular Medicine, McMaster UniversityDepartment of Anesthesia, McMaster UniversityDepartment of Surgery, McMaster UniversityW Booth School of Engineering Practice and Technology, McMaster UniversityAbstract Unraveling the intricate relationship between mechanical factors and brain activity is a pivotal endeavor, yet the underlying mechanistic model of signaling pathways in brain mechanotransduction remains enigmatic. To bridge this gap, we introduced an in situ multi-scale platform, through which we delineate comprehensive brain biomechanical traits in white matter (WM), grey-white matter junctions (GW junction), and the pons across human brain tissue from four distinct donors. We investigate the three-dimensional expression patterns of Piezo1, Piezo2, and TMEM150C, while also examining their associated histological features and mechanotransduction signaling networks, particularly focusing on the YAP/β-catenin axis. Our results showed that the biomechanical characteristics (including stiffness, spring term, and equilibrium stress) associated with Piezo1 vary depending on the specific region. Moving beyond Piezo1, our result demonstrated the significant positive correlations between Piezo2 expression and stiffness in the WM. Meanwhile, the expression of Piezo2 and TMEM150C was shown to be correlated to viscoelastic properties in the pons and WM. Given the heterogeneity of brain tissue, we investigated the three-dimensional expression of Piezo1, Piezo2, and TMEM150C. Our results suggested that three mechanosensitive proteins remained consistent across different vertical planes within the tissue sections. Our findings not only establish Piezo1, Piezo2, and TMEM150C as pivotal mechanosensors that regulate the region-specific mechanotransduction activities but also unveil the paradigm connecting brain mechanical properties and mechanotransduction activities and the variations between individuals.https://doi.org/10.1186/s13041-023-01071-5Brain biomechanicsCellular mechanotransductionPiezo1Piezo2TMEM150CViscoelastic properties |
| spellingShingle | Arjun Raha Yuning Wu Lily Zhong Jatheeshan Raveenthiran Minji Hong Aftab Taiyab Li Wang Bill Wang Fei Geng Exploring Piezo1, Piezo2, and TMEM150C in human brain tissues and their correlation with brain biomechanical characteristics Molecular Brain Brain biomechanics Cellular mechanotransduction Piezo1 Piezo2 TMEM150C Viscoelastic properties |
| title | Exploring Piezo1, Piezo2, and TMEM150C in human brain tissues and their correlation with brain biomechanical characteristics |
| title_full | Exploring Piezo1, Piezo2, and TMEM150C in human brain tissues and their correlation with brain biomechanical characteristics |
| title_fullStr | Exploring Piezo1, Piezo2, and TMEM150C in human brain tissues and their correlation with brain biomechanical characteristics |
| title_full_unstemmed | Exploring Piezo1, Piezo2, and TMEM150C in human brain tissues and their correlation with brain biomechanical characteristics |
| title_short | Exploring Piezo1, Piezo2, and TMEM150C in human brain tissues and their correlation with brain biomechanical characteristics |
| title_sort | exploring piezo1 piezo2 and tmem150c in human brain tissues and their correlation with brain biomechanical characteristics |
| topic | Brain biomechanics Cellular mechanotransduction Piezo1 Piezo2 TMEM150C Viscoelastic properties |
| url | https://doi.org/10.1186/s13041-023-01071-5 |
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