| Summary: | We report here the AflR binding motif of <i>Aspergillus flavus</i> for the first time with the aid of ChIP-seq analysis. Of the 540 peak sequences associated with AflR binding events, 66.8% were located within 2 kb upstream (promoter region) of translational start sites. The identified 18-bp binding motif was a perfect palindromic sequence, 5′-CSSGGGWTCGAWCCCSSG’3′ with S representing G or C and W representing A or T. On closer examination, we hypothesized that the 18-bp motif sequence identified contained two identical parts (here called motif A and motif B). Motif A was in positions 8–18 on the upper strand, while motif B was in positions 11-1 on the bottom strand. The inferred length and sequence of the putative motif identified in <i>A. flavus</i> were similar to previous findings in <i>A. parasiticus</i> and <i>A. nidulans</i>. Gene ontology analysis indicated that AflR bound to other genes outside the aflatoxin biosynthetic gene cluster.
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