Xylanase Production by a Local Isolate, Trichoderma spp. FETL c3-2 via Solid State Fermentation Using Agricultural Wastes as Substrates
The production of xylanase by a local isolate Trichoderma spp. FETL c3-2 via solid state fermentation system using sugar cane baggase:palm kernel cake as substrates was investigated. The optimized solid state fermentation (SSF) system consists of 5 g of sugar cane baggase: palm kernel cake of rati...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Malaysian Society for Microbiology
2006-01-01
|
| Series: | Malaysian Journal of Microbiology |
| Subjects: | |
| Online Access: | http://web.usm.my/mjm/issues/vol2no1/research2.pdf |
| _version_ | 1828359867722629120 |
|---|---|
| author | Pang, P. K. Darah, I. Poppe, L. Szakacs, G. Ibrahim, C. O. |
| author_facet | Pang, P. K. Darah, I. Poppe, L. Szakacs, G. Ibrahim, C. O. |
| author_sort | Pang, P. K. |
| collection | DOAJ |
| description | The production of xylanase by a local isolate Trichoderma spp. FETL c3-2 via solid state fermentation system using sugar cane baggase:palm kernel cake as substrates was investigated. The optimized solid state fermentation (SSF) system consists of 5 g of sugar cane baggase: palm kernel cake of ratio 90:10 (%, w/w), moisture content of 75% (v/w), pH of moistening agent of pH 7.0, at 30C and inoculum size of 1x10^8 spores/ml. The SSF system was also supplemented with 4% (w/w) dextrin and 6% (w/w) of tryptone as additional carbon and nitrogen sources, respectively. Cellulose at the concentration of 0.2% (w/w) was found to be a significant inducer for xylanase production. Using the optimized SSF system, a maximum xylanase productivity of 75.0 U per mg glucosamine after 4 days of fermentation time at 30C was obtained. The modifications of the SSF system resulted an increase in xylanase productivity by 180% and growth by 40% compared to the basal SSF system. |
| first_indexed | 2024-04-14T03:48:16Z |
| format | Article |
| id | doaj.art-d81fd84472ea4f6dad775dacc7845609 |
| institution | Directory Open Access Journal |
| issn | 1823-8262 2231-7538 |
| language | English |
| last_indexed | 2024-04-14T03:48:16Z |
| publishDate | 2006-01-01 |
| publisher | Malaysian Society for Microbiology |
| record_format | Article |
| series | Malaysian Journal of Microbiology |
| spelling | doaj.art-d81fd84472ea4f6dad775dacc78456092022-12-22T02:14:10ZengMalaysian Society for MicrobiologyMalaysian Journal of Microbiology1823-82622231-75382006-01-0121714Xylanase Production by a Local Isolate, Trichoderma spp. FETL c3-2 via Solid State Fermentation Using Agricultural Wastes as SubstratesPang, P. K.Darah, I.Poppe, L.Szakacs, G.Ibrahim, C. O.The production of xylanase by a local isolate Trichoderma spp. FETL c3-2 via solid state fermentation system using sugar cane baggase:palm kernel cake as substrates was investigated. The optimized solid state fermentation (SSF) system consists of 5 g of sugar cane baggase: palm kernel cake of ratio 90:10 (%, w/w), moisture content of 75% (v/w), pH of moistening agent of pH 7.0, at 30C and inoculum size of 1x10^8 spores/ml. The SSF system was also supplemented with 4% (w/w) dextrin and 6% (w/w) of tryptone as additional carbon and nitrogen sources, respectively. Cellulose at the concentration of 0.2% (w/w) was found to be a significant inducer for xylanase production. Using the optimized SSF system, a maximum xylanase productivity of 75.0 U per mg glucosamine after 4 days of fermentation time at 30C was obtained. The modifications of the SSF system resulted an increase in xylanase productivity by 180% and growth by 40% compared to the basal SSF system.http://web.usm.my/mjm/issues/vol2no1/research2.pdfXylanase productionSolid state fermentationTrichoderma spp.Agricultural wastes |
| spellingShingle | Pang, P. K. Darah, I. Poppe, L. Szakacs, G. Ibrahim, C. O. Xylanase Production by a Local Isolate, Trichoderma spp. FETL c3-2 via Solid State Fermentation Using Agricultural Wastes as Substrates Malaysian Journal of Microbiology Xylanase production Solid state fermentation Trichoderma spp. Agricultural wastes |
| title | Xylanase Production by a Local Isolate, Trichoderma spp. FETL c3-2 via Solid State Fermentation Using Agricultural Wastes as Substrates |
| title_full | Xylanase Production by a Local Isolate, Trichoderma spp. FETL c3-2 via Solid State Fermentation Using Agricultural Wastes as Substrates |
| title_fullStr | Xylanase Production by a Local Isolate, Trichoderma spp. FETL c3-2 via Solid State Fermentation Using Agricultural Wastes as Substrates |
| title_full_unstemmed | Xylanase Production by a Local Isolate, Trichoderma spp. FETL c3-2 via Solid State Fermentation Using Agricultural Wastes as Substrates |
| title_short | Xylanase Production by a Local Isolate, Trichoderma spp. FETL c3-2 via Solid State Fermentation Using Agricultural Wastes as Substrates |
| title_sort | xylanase production by a local isolate trichoderma spp fetl c3 2 via solid state fermentation using agricultural wastes as substrates |
| topic | Xylanase production Solid state fermentation Trichoderma spp. Agricultural wastes |
| url | http://web.usm.my/mjm/issues/vol2no1/research2.pdf |
| work_keys_str_mv | AT pangpk xylanaseproductionbyalocalisolatetrichodermasppfetlc32viasolidstatefermentationusingagriculturalwastesassubstrates AT darahi xylanaseproductionbyalocalisolatetrichodermasppfetlc32viasolidstatefermentationusingagriculturalwastesassubstrates AT poppel xylanaseproductionbyalocalisolatetrichodermasppfetlc32viasolidstatefermentationusingagriculturalwastesassubstrates AT szakacsg xylanaseproductionbyalocalisolatetrichodermasppfetlc32viasolidstatefermentationusingagriculturalwastesassubstrates AT ibrahimco xylanaseproductionbyalocalisolatetrichodermasppfetlc32viasolidstatefermentationusingagriculturalwastesassubstrates |