Feline sporotrichosis: associations between clinical-epidemiological profiles and phenotypic-genotypic characteristics of the etiological agents in the Rio de Janeiro epizootic area
BACKGROUND Sporotrichosis is caused by species of the genus Sporothrix. From 1998 to 2015, 4,703 cats were diagnosed at the Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil. Even after the description of the Sporothrix species, the characterisation of feline isolates is not performed routinel...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000300185&lng=en&tlng=en |
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| author | Jéssica Sepulveda Boechat Manoel Marques Evangelista Oliveira Rodrigo Almeida-Paes Isabella Dib Ferreira Gremião Ana Caroline de Sá Machado Raquel de Vasconcelos Carvalhaes Oliveira Anna Barreto Fernandes Figueiredo Vanessa Brito de Souza Rabello Karoline Benevides de Lima Silva Rosely Maria Zancopé-Oliveira Tânia Maria Pacheco Schubach Sandro Antonio Pereira |
| author_facet | Jéssica Sepulveda Boechat Manoel Marques Evangelista Oliveira Rodrigo Almeida-Paes Isabella Dib Ferreira Gremião Ana Caroline de Sá Machado Raquel de Vasconcelos Carvalhaes Oliveira Anna Barreto Fernandes Figueiredo Vanessa Brito de Souza Rabello Karoline Benevides de Lima Silva Rosely Maria Zancopé-Oliveira Tânia Maria Pacheco Schubach Sandro Antonio Pereira |
| author_sort | Jéssica Sepulveda Boechat |
| collection | DOAJ |
| description | BACKGROUND Sporotrichosis is caused by species of the genus Sporothrix. From 1998 to 2015, 4,703 cats were diagnosed at the Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil. Even after the description of the Sporothrix species, the characterisation of feline isolates is not performed routinely. OBJECTIVES To characterise the clinical isolates from cats at the species level and correlate them with the clinical and epidemiological characteristics of the cats. METHODS Forty seven Sporothrix spp. isolates from cats assisted at Fiocruz from 2010 to 2011 were included. Medical records were consulted to obtain the clinical and epidemiological data. The isolates were identified through their morphological and physiological characteristics. T3B polymerase chain reaction (PCR) fingerprinting was used for molecular identification of the species. FINDINGS In phenotypic tests, 34 isolates were characterised as S. brasiliensis, one as S. schenckii and 12 as Sporothrix spp. PCR identified all isolates as S. brasiliensis. MAIN CONCLUSIONS S. brasiliensis is the only etiological agent of feline sporotrichosis in Rio de Janeiro to date. None association was found between the isolates and the clinical and epidemiological data. In addition, we strongly recommend the use of molecular techniques for the identification of isolates of Sporothrix spp. |
| first_indexed | 2024-03-12T18:18:37Z |
| format | Article |
| id | doaj.art-d82566aa398344af8e412084da8154c1 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:18:37Z |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-d82566aa398344af8e412084da8154c12023-08-02T09:00:11ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-8060113318519610.1590/0074-02760170407S0074-02762018000300185Feline sporotrichosis: associations between clinical-epidemiological profiles and phenotypic-genotypic characteristics of the etiological agents in the Rio de Janeiro epizootic areaJéssica Sepulveda BoechatManoel Marques Evangelista OliveiraRodrigo Almeida-PaesIsabella Dib Ferreira GremiãoAna Caroline de Sá MachadoRaquel de Vasconcelos Carvalhaes OliveiraAnna Barreto Fernandes FigueiredoVanessa Brito de Souza RabelloKaroline Benevides de Lima SilvaRosely Maria Zancopé-OliveiraTânia Maria Pacheco SchubachSandro Antonio PereiraBACKGROUND Sporotrichosis is caused by species of the genus Sporothrix. From 1998 to 2015, 4,703 cats were diagnosed at the Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil. Even after the description of the Sporothrix species, the characterisation of feline isolates is not performed routinely. OBJECTIVES To characterise the clinical isolates from cats at the species level and correlate them with the clinical and epidemiological characteristics of the cats. METHODS Forty seven Sporothrix spp. isolates from cats assisted at Fiocruz from 2010 to 2011 were included. Medical records were consulted to obtain the clinical and epidemiological data. The isolates were identified through their morphological and physiological characteristics. T3B polymerase chain reaction (PCR) fingerprinting was used for molecular identification of the species. FINDINGS In phenotypic tests, 34 isolates were characterised as S. brasiliensis, one as S. schenckii and 12 as Sporothrix spp. PCR identified all isolates as S. brasiliensis. MAIN CONCLUSIONS S. brasiliensis is the only etiological agent of feline sporotrichosis in Rio de Janeiro to date. None association was found between the isolates and the clinical and epidemiological data. In addition, we strongly recommend the use of molecular techniques for the identification of isolates of Sporothrix spp.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000300185&lng=en&tlng=enSporothrix speciessporotrichosiscatsphenotypic characterisationmolecular characterisation |
| spellingShingle | Jéssica Sepulveda Boechat Manoel Marques Evangelista Oliveira Rodrigo Almeida-Paes Isabella Dib Ferreira Gremião Ana Caroline de Sá Machado Raquel de Vasconcelos Carvalhaes Oliveira Anna Barreto Fernandes Figueiredo Vanessa Brito de Souza Rabello Karoline Benevides de Lima Silva Rosely Maria Zancopé-Oliveira Tânia Maria Pacheco Schubach Sandro Antonio Pereira Feline sporotrichosis: associations between clinical-epidemiological profiles and phenotypic-genotypic characteristics of the etiological agents in the Rio de Janeiro epizootic area Memorias do Instituto Oswaldo Cruz Sporothrix species sporotrichosis cats phenotypic characterisation molecular characterisation |
| title | Feline sporotrichosis: associations between clinical-epidemiological profiles and phenotypic-genotypic characteristics of the etiological agents in the Rio de Janeiro epizootic area |
| title_full | Feline sporotrichosis: associations between clinical-epidemiological profiles and phenotypic-genotypic characteristics of the etiological agents in the Rio de Janeiro epizootic area |
| title_fullStr | Feline sporotrichosis: associations between clinical-epidemiological profiles and phenotypic-genotypic characteristics of the etiological agents in the Rio de Janeiro epizootic area |
| title_full_unstemmed | Feline sporotrichosis: associations between clinical-epidemiological profiles and phenotypic-genotypic characteristics of the etiological agents in the Rio de Janeiro epizootic area |
| title_short | Feline sporotrichosis: associations between clinical-epidemiological profiles and phenotypic-genotypic characteristics of the etiological agents in the Rio de Janeiro epizootic area |
| title_sort | feline sporotrichosis associations between clinical epidemiological profiles and phenotypic genotypic characteristics of the etiological agents in the rio de janeiro epizootic area |
| topic | Sporothrix species sporotrichosis cats phenotypic characterisation molecular characterisation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000300185&lng=en&tlng=en |
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