Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples
Gradient images can be obtained using a rotating square mask to filter the angular spectra of the wavefront generated by a complex transmittance object. This method can be applied to measure the three-dimensional structure of microscopic biological samples through the relationship of the phase with...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/22/5/1842 |
| _version_ | 1797473817794183168 |
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| author | Ignacio Iglesias |
| author_facet | Ignacio Iglesias |
| author_sort | Ignacio Iglesias |
| collection | DOAJ |
| description | Gradient images can be obtained using a rotating square mask to filter the angular spectra of the wavefront generated by a complex transmittance object. This method can be applied to measure the three-dimensional structure of microscopic biological samples through the relationship of the phase with the optical path length. This work describes the implementation of a system using an inverted optical microscope and shows the experimental results of phase maps generated by boar sperm cells. |
| first_indexed | 2024-03-09T20:21:45Z |
| format | Article |
| id | doaj.art-d825895583f0426eb9c8739e8a739189 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T20:21:45Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-d825895583f0426eb9c8739e8a7391892023-11-23T23:47:04ZengMDPI AGSensors1424-82202022-02-01225184210.3390/s22051842Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological SamplesIgnacio Iglesias0Departamento de Física, CEIR Campus Mare Nostrum (CMN), Universidad de Murcia, 30100 Murcia, SpainGradient images can be obtained using a rotating square mask to filter the angular spectra of the wavefront generated by a complex transmittance object. This method can be applied to measure the three-dimensional structure of microscopic biological samples through the relationship of the phase with the optical path length. This work describes the implementation of a system using an inverted optical microscope and shows the experimental results of phase maps generated by boar sperm cells.https://www.mdpi.com/1424-8220/22/5/1842microscopyquantitative microscopywavefront sensing |
| spellingShingle | Ignacio Iglesias Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples Sensors microscopy quantitative microscopy wavefront sensing |
| title | Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples |
| title_full | Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples |
| title_fullStr | Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples |
| title_full_unstemmed | Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples |
| title_short | Use of a Rotating Square Spatial-Frequency Filter to Map the Optical Path Length Variation in Microscopic Biological Samples |
| title_sort | use of a rotating square spatial frequency filter to map the optical path length variation in microscopic biological samples |
| topic | microscopy quantitative microscopy wavefront sensing |
| url | https://www.mdpi.com/1424-8220/22/5/1842 |
| work_keys_str_mv | AT ignacioiglesias useofarotatingsquarespatialfrequencyfiltertomaptheopticalpathlengthvariationinmicroscopicbiologicalsamples |