No Association of Folate- Related and Methionine Synthesis Genes Variant in the Development and Progression of Childhood ALL among North Indian Population
Introduction: Acute Lymphoblastic Leukemia (ALL) is the most worldwide common type of childhood cancer. Methylenetetrahydrofolate reductase (MTHFR) and 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) are crucial enzymes in folate pathways. Folate availability is critical factor for DNA...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2011-01-01
|
| Series: | International Journal of Hematology-Oncology and Stem Cell Research |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/upload_files/pdf/19319.pdf |
| _version_ | 1827884709316657152 |
|---|---|
| author | Marwaha Ram Kumar Deepak Kaul Neena Capalash Marjan Askari Kianoosh MalekZadeh Jha Abhimanyu Kumar Mohsen Nikbakht Jagdeep Kaur |
| author_facet | Marwaha Ram Kumar Deepak Kaul Neena Capalash Marjan Askari Kianoosh MalekZadeh Jha Abhimanyu Kumar Mohsen Nikbakht Jagdeep Kaur |
| author_sort | Marwaha Ram Kumar |
| collection | DOAJ |
| description | Introduction: Acute Lymphoblastic Leukemia (ALL) is the most worldwide common type of childhood cancer. Methylenetetrahydrofolate reductase (MTHFR) and 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) are crucial enzymes in folate pathways. Folate availability is critical factor for DNA integrity, required for the transfer of methyl group in the biosynthesis of thymidilate.Procedure: In present study, we have conducted a case control study from north Indian states to correlate the effect of two SNPs of MTHFR (677C→T and 1298A→C) and MTR (2756A→G) and the risk of childhood ALL. One hundred and twenty five bone marrows and peripheral blood samples and 100 sex-age matched healthy controls were obtained and analyzed by PCR-RFLP method. Results: Statistically, no significant differences were observed between patients and controls for different genotypes (p>0.05), also significant different on risk of ALL in individuals having genotype of MTHFR 677TT (OR=0.61, 95% CI=0.21-1.77) and MTHFR 1298CC (OR=0.56, 95% CI=0.18-1.68) was not observed. Statistically, the correlation of variants of MTR gene and risk of ALL was not observed. Conclusions: The difference in distribution of possible combined genotypes of MTHFR (677C→T, 1298A→C) and MTR (2756A→G) between patients and controls were statistically insignificant. |
| first_indexed | 2024-03-12T19:33:54Z |
| format | Article |
| id | doaj.art-d825a92bccd64d76be4ceeb615763e73 |
| institution | Directory Open Access Journal |
| issn | 2008-3009 2008-2207 |
| language | English |
| last_indexed | 2024-03-12T19:33:54Z |
| publishDate | 2011-01-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | International Journal of Hematology-Oncology and Stem Cell Research |
| spelling | doaj.art-d825a92bccd64d76be4ceeb615763e732023-08-02T04:20:44ZengTehran University of Medical SciencesInternational Journal of Hematology-Oncology and Stem Cell Research2008-30092008-22072011-01-01512029No Association of Folate- Related and Methionine Synthesis Genes Variant in the Development and Progression of Childhood ALL among North Indian PopulationMarwaha Ram KumarDeepak KaulNeena CapalashMarjan AskariKianoosh MalekZadehJha Abhimanyu KumarMohsen NikbakhtJagdeep KaurIntroduction: Acute Lymphoblastic Leukemia (ALL) is the most worldwide common type of childhood cancer. Methylenetetrahydrofolate reductase (MTHFR) and 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) are crucial enzymes in folate pathways. Folate availability is critical factor for DNA integrity, required for the transfer of methyl group in the biosynthesis of thymidilate.Procedure: In present study, we have conducted a case control study from north Indian states to correlate the effect of two SNPs of MTHFR (677C→T and 1298A→C) and MTR (2756A→G) and the risk of childhood ALL. One hundred and twenty five bone marrows and peripheral blood samples and 100 sex-age matched healthy controls were obtained and analyzed by PCR-RFLP method. Results: Statistically, no significant differences were observed between patients and controls for different genotypes (p>0.05), also significant different on risk of ALL in individuals having genotype of MTHFR 677TT (OR=0.61, 95% CI=0.21-1.77) and MTHFR 1298CC (OR=0.56, 95% CI=0.18-1.68) was not observed. Statistically, the correlation of variants of MTR gene and risk of ALL was not observed. Conclusions: The difference in distribution of possible combined genotypes of MTHFR (677C→T, 1298A→C) and MTR (2756A→G) between patients and controls were statistically insignificant.http://journals.tums.ac.ir/upload_files/pdf/19319.pdfMethylenetetrahydrofolate Reductase (MTHFR)5-Methyltetrahydrofolate-Homocysteine Methyltransferase (MTR)Acute Lymphoblastic Leukemia (ALL)Hypomethylation |
| spellingShingle | Marwaha Ram Kumar Deepak Kaul Neena Capalash Marjan Askari Kianoosh MalekZadeh Jha Abhimanyu Kumar Mohsen Nikbakht Jagdeep Kaur No Association of Folate- Related and Methionine Synthesis Genes Variant in the Development and Progression of Childhood ALL among North Indian Population International Journal of Hematology-Oncology and Stem Cell Research Methylenetetrahydrofolate Reductase (MTHFR) 5-Methyltetrahydrofolate-Homocysteine Methyltransferase (MTR) Acute Lymphoblastic Leukemia (ALL) Hypomethylation |
| title | No Association of Folate- Related and Methionine Synthesis Genes Variant in the Development and Progression of Childhood ALL among North Indian Population |
| title_full | No Association of Folate- Related and Methionine Synthesis Genes Variant in the Development and Progression of Childhood ALL among North Indian Population |
| title_fullStr | No Association of Folate- Related and Methionine Synthesis Genes Variant in the Development and Progression of Childhood ALL among North Indian Population |
| title_full_unstemmed | No Association of Folate- Related and Methionine Synthesis Genes Variant in the Development and Progression of Childhood ALL among North Indian Population |
| title_short | No Association of Folate- Related and Methionine Synthesis Genes Variant in the Development and Progression of Childhood ALL among North Indian Population |
| title_sort | no association of folate related and methionine synthesis genes variant in the development and progression of childhood all among north indian population |
| topic | Methylenetetrahydrofolate Reductase (MTHFR) 5-Methyltetrahydrofolate-Homocysteine Methyltransferase (MTR) Acute Lymphoblastic Leukemia (ALL) Hypomethylation |
| url | http://journals.tums.ac.ir/upload_files/pdf/19319.pdf |
| work_keys_str_mv | AT marwaharamkumar noassociationoffolaterelatedandmethioninesynthesisgenesvariantinthedevelopmentandprogressionofchildhoodallamongnorthindianpopulation AT deepakkaul noassociationoffolaterelatedandmethioninesynthesisgenesvariantinthedevelopmentandprogressionofchildhoodallamongnorthindianpopulation AT neenacapalash noassociationoffolaterelatedandmethioninesynthesisgenesvariantinthedevelopmentandprogressionofchildhoodallamongnorthindianpopulation AT marjanaskari noassociationoffolaterelatedandmethioninesynthesisgenesvariantinthedevelopmentandprogressionofchildhoodallamongnorthindianpopulation AT kianooshmalekzadeh noassociationoffolaterelatedandmethioninesynthesisgenesvariantinthedevelopmentandprogressionofchildhoodallamongnorthindianpopulation AT jhaabhimanyukumar noassociationoffolaterelatedandmethioninesynthesisgenesvariantinthedevelopmentandprogressionofchildhoodallamongnorthindianpopulation AT mohsennikbakht noassociationoffolaterelatedandmethioninesynthesisgenesvariantinthedevelopmentandprogressionofchildhoodallamongnorthindianpopulation AT jagdeepkaur noassociationoffolaterelatedandmethioninesynthesisgenesvariantinthedevelopmentandprogressionofchildhoodallamongnorthindianpopulation |