Mesenchymal stem cells under hypoxia condition inhibit peritoneal adhesion by suppressing the prolonged release of interleukin-6
Aim To investigate the role of hypoxia-preconditioned mesenchymal stem cells (H-MSCs) in preventing peritoneal adhesion by regulating IL-6 at days 6 and 12. Methods Twenty-four PAs rat model weighing 250 g to 300 g were randomly allocated into 4 groups: sham (Sh), control (C), H-MSCs treatment g...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Medical Association of Zenica-Doboj Canton
2021-08-01
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| Series: | Medicinski Glasnik |
| Subjects: | |
| Online Access: | https://ljkzedo.ba/mgpdf/mg35/20_Muhar_1304_A.pdf |
| Summary: | Aim To investigate the role of hypoxia-preconditioned mesenchymal stem cells (H-MSCs) in preventing peritoneal adhesion
by regulating IL-6 at days 6 and 12.
Methods Twenty-four PAs rat model weighing 250 g to 300 g
were randomly allocated into 4 groups: sham (Sh), control (C),
H-MSCs treatment group at dose 1.5 x 106
(T1) and 3 x 106
(T2).
To induce H-MSCs, all MSCs population were incubated under
hypoxia state (5% O2
), 5% CO2
, and 37o C for 24 hours. Expression level of IL-6 was performed using ELISA. Morphological
appearance of adhesion was observed by visualizing the existence
of adhesion formation in intestinal.
Results In this study we found that there was a trend of decrease
of IL-6 level on day 6 following MSCs treatments. Interestingly,
there was a significant decrease of IL-6 level on day 12 in all treatment groups. Also, no adhesion occurred in T2 group.
Conclusions H-MSCs prevent PA development by suppressing
the prolonged release of IL-6 at proliferation phase. |
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| ISSN: | 1840-0132 1840-2445 |