Mesenchymal stem cells under hypoxia condition inhibit peritoneal adhesion by suppressing the prolonged release of interleukin-6
Aim To investigate the role of hypoxia-preconditioned mesenchymal stem cells (H-MSCs) in preventing peritoneal adhesion by regulating IL-6 at days 6 and 12. Methods Twenty-four PAs rat model weighing 250 g to 300 g were randomly allocated into 4 groups: sham (Sh), control (C), H-MSCs treatment g...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Medical Association of Zenica-Doboj Canton
2021-08-01
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| Series: | Medicinski Glasnik |
| Subjects: | |
| Online Access: | https://ljkzedo.ba/mgpdf/mg35/20_Muhar_1304_A.pdf |
| _version_ | 1797738422057566208 |
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| author | Adi Muradi Muhar Agung Putra Vito Mahendra Ekasaputra Dewi Masyithah Darlan Desiree Anggia Paramita Iqbal Pahlevi Adeputra Nasution |
| author_facet | Adi Muradi Muhar Agung Putra Vito Mahendra Ekasaputra Dewi Masyithah Darlan Desiree Anggia Paramita Iqbal Pahlevi Adeputra Nasution |
| author_sort | Adi Muradi Muhar |
| collection | DOAJ |
| description | Aim To investigate the role of hypoxia-preconditioned mesenchymal stem cells (H-MSCs) in preventing peritoneal adhesion
by regulating IL-6 at days 6 and 12.
Methods Twenty-four PAs rat model weighing 250 g to 300 g
were randomly allocated into 4 groups: sham (Sh), control (C),
H-MSCs treatment group at dose 1.5 x 106
(T1) and 3 x 106
(T2).
To induce H-MSCs, all MSCs population were incubated under
hypoxia state (5% O2
), 5% CO2
, and 37o C for 24 hours. Expression level of IL-6 was performed using ELISA. Morphological
appearance of adhesion was observed by visualizing the existence
of adhesion formation in intestinal.
Results In this study we found that there was a trend of decrease
of IL-6 level on day 6 following MSCs treatments. Interestingly,
there was a significant decrease of IL-6 level on day 12 in all treatment groups. Also, no adhesion occurred in T2 group.
Conclusions H-MSCs prevent PA development by suppressing
the prolonged release of IL-6 at proliferation phase. |
| first_indexed | 2024-03-12T13:42:36Z |
| format | Article |
| id | doaj.art-d8266478648b46739353ace167c4a45f |
| institution | Directory Open Access Journal |
| issn | 1840-0132 1840-2445 |
| language | English |
| last_indexed | 2024-03-12T13:42:36Z |
| publishDate | 2021-08-01 |
| publisher | Medical Association of Zenica-Doboj Canton |
| record_format | Article |
| series | Medicinski Glasnik |
| spelling | doaj.art-d8266478648b46739353ace167c4a45f2023-08-23T13:55:26ZengMedical Association of Zenica-Doboj CantonMedicinski Glasnik1840-01321840-24452021-08-0118239840310.17392/1304-21Mesenchymal stem cells under hypoxia condition inhibit peritoneal adhesion by suppressing the prolonged release of interleukin-6Adi Muradi Muhar0Agung Putra1Vito Mahendra Ekasaputra2Dewi Masyithah Darlan3Desiree Anggia Paramita4Iqbal Pahlevi Adeputra Nasution5Department of Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, North SumateraStem Cell and Cancer Research (SCCR), Medical Faculty, Universitas Islam Sultan Agung (Unissula), Semarang, Central Java; Department of Postgraduate Biomedical Science, Medical Faculty, Universitas Islam Sultan Agung (Unissula), Semarang, Central Java; Department of Pathological Anatomy, Medical Faculty, Universitas Islam Sultan Agung (Unissula), Semarang, Central JavaDepartment of Surgery, Medical Faculty, Universitas Islam Sultan Agung (Unissula), Semarang, Central JavaDepartment of Parasitology, Faculty of Medicine, Universitas Sumatera Utara, Medan, North Sumatera; IndonesiaDepartment of Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, North SumateraDepartment of Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, North SumateraAim To investigate the role of hypoxia-preconditioned mesenchymal stem cells (H-MSCs) in preventing peritoneal adhesion by regulating IL-6 at days 6 and 12. Methods Twenty-four PAs rat model weighing 250 g to 300 g were randomly allocated into 4 groups: sham (Sh), control (C), H-MSCs treatment group at dose 1.5 x 106 (T1) and 3 x 106 (T2). To induce H-MSCs, all MSCs population were incubated under hypoxia state (5% O2 ), 5% CO2 , and 37o C for 24 hours. Expression level of IL-6 was performed using ELISA. Morphological appearance of adhesion was observed by visualizing the existence of adhesion formation in intestinal. Results In this study we found that there was a trend of decrease of IL-6 level on day 6 following MSCs treatments. Interestingly, there was a significant decrease of IL-6 level on day 12 in all treatment groups. Also, no adhesion occurred in T2 group. Conclusions H-MSCs prevent PA development by suppressing the prolonged release of IL-6 at proliferation phase.https://ljkzedo.ba/mgpdf/mg35/20_Muhar_1304_A.pdfabdominal painabdominal surgerycellularinflammatory cytokines |
| spellingShingle | Adi Muradi Muhar Agung Putra Vito Mahendra Ekasaputra Dewi Masyithah Darlan Desiree Anggia Paramita Iqbal Pahlevi Adeputra Nasution Mesenchymal stem cells under hypoxia condition inhibit peritoneal adhesion by suppressing the prolonged release of interleukin-6 Medicinski Glasnik abdominal pain abdominal surgery cellular inflammatory cytokines |
| title | Mesenchymal stem cells under hypoxia condition inhibit peritoneal adhesion by suppressing the prolonged release of interleukin-6 |
| title_full | Mesenchymal stem cells under hypoxia condition inhibit peritoneal adhesion by suppressing the prolonged release of interleukin-6 |
| title_fullStr | Mesenchymal stem cells under hypoxia condition inhibit peritoneal adhesion by suppressing the prolonged release of interleukin-6 |
| title_full_unstemmed | Mesenchymal stem cells under hypoxia condition inhibit peritoneal adhesion by suppressing the prolonged release of interleukin-6 |
| title_short | Mesenchymal stem cells under hypoxia condition inhibit peritoneal adhesion by suppressing the prolonged release of interleukin-6 |
| title_sort | mesenchymal stem cells under hypoxia condition inhibit peritoneal adhesion by suppressing the prolonged release of interleukin 6 |
| topic | abdominal pain abdominal surgery cellular inflammatory cytokines |
| url | https://ljkzedo.ba/mgpdf/mg35/20_Muhar_1304_A.pdf |
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