Regulation of Drought and Salt Tolerance by OsSKL2 and OsASR1 in Rice
Abstract Abiotic stresses such as salinity and drought greatly impact the growth and production of crops worldwide. Here, a shikimate kinase-like 2 (SKL2) gene was cloned from rice and characterized for its regulatory function in salinity and drought tolerance. OsSKL2 was localized in the chloroplas...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2022-08-01
|
| Series: | Rice |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12284-022-00592-2 |
| _version_ | 1798037345212039168 |
|---|---|
| author | Yingli Jiang Xiaojian Peng Qin Zhang Yuqing Liu Aiqi Li Beijiu Cheng Jiandong Wu |
| author_facet | Yingli Jiang Xiaojian Peng Qin Zhang Yuqing Liu Aiqi Li Beijiu Cheng Jiandong Wu |
| author_sort | Yingli Jiang |
| collection | DOAJ |
| description | Abstract Abiotic stresses such as salinity and drought greatly impact the growth and production of crops worldwide. Here, a shikimate kinase-like 2 (SKL2) gene was cloned from rice and characterized for its regulatory function in salinity and drought tolerance. OsSKL2 was localized in the chloroplast, and its transcripts were significantly induced by drought and salinity stress as well as H2O2 and abscisic acid (ABA) treatment. Meanwhile, overexpression of OsSKL2 in rice increased tolerance to salinity, drought and oxidative stress by increasing antioxidant enzyme activity, and reducing levels of H2O2, malondialdehyde, and relative electrolyte leakage. In contrast, RNAi-induced suppression of OsSKL2 increased sensitivity to stress treatment. Interestingly, overexpression of OsSKL2 also increased sensitivity to exogenous ABA, with an increase in reactive oxygen species (ROS) accumulation. Moreover, OsSKL2 was found to physically interact with OsASR1, a well-known chaperone-like protein, which also exhibited positive roles in salt and drought tolerance. A reduction in ROS production was also observed in leaves of Nicotiana benthamiana showing transient co-expression of OsSKL2 with OsASR1. Taken together, these findings suggest that OsSKL2 together with OsASR1 act as important regulatory factors that confer salt and drought tolerance in rice via ROS scavenging. |
| first_indexed | 2024-04-11T21:25:18Z |
| format | Article |
| id | doaj.art-d826d752575349a4b83abe2aa78229c3 |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-04-11T21:25:18Z |
| publishDate | 2022-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-d826d752575349a4b83abe2aa78229c32022-12-22T04:02:28ZengSpringerOpenRice1939-84251939-84332022-08-0115111710.1186/s12284-022-00592-2Regulation of Drought and Salt Tolerance by OsSKL2 and OsASR1 in RiceYingli Jiang0Xiaojian Peng1Qin Zhang2Yuqing Liu3Aiqi Li4Beijiu Cheng5Jiandong Wu6National Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural UniversityNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural UniversityNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural UniversityNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural UniversityNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural UniversityNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural UniversityNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural UniversityAbstract Abiotic stresses such as salinity and drought greatly impact the growth and production of crops worldwide. Here, a shikimate kinase-like 2 (SKL2) gene was cloned from rice and characterized for its regulatory function in salinity and drought tolerance. OsSKL2 was localized in the chloroplast, and its transcripts were significantly induced by drought and salinity stress as well as H2O2 and abscisic acid (ABA) treatment. Meanwhile, overexpression of OsSKL2 in rice increased tolerance to salinity, drought and oxidative stress by increasing antioxidant enzyme activity, and reducing levels of H2O2, malondialdehyde, and relative electrolyte leakage. In contrast, RNAi-induced suppression of OsSKL2 increased sensitivity to stress treatment. Interestingly, overexpression of OsSKL2 also increased sensitivity to exogenous ABA, with an increase in reactive oxygen species (ROS) accumulation. Moreover, OsSKL2 was found to physically interact with OsASR1, a well-known chaperone-like protein, which also exhibited positive roles in salt and drought tolerance. A reduction in ROS production was also observed in leaves of Nicotiana benthamiana showing transient co-expression of OsSKL2 with OsASR1. Taken together, these findings suggest that OsSKL2 together with OsASR1 act as important regulatory factors that confer salt and drought tolerance in rice via ROS scavenging.https://doi.org/10.1186/s12284-022-00592-2Salt and drought toleranceOryza sativaOsSKL2Reactive oxygen speciesProtein interactionsOsASR1 |
| spellingShingle | Yingli Jiang Xiaojian Peng Qin Zhang Yuqing Liu Aiqi Li Beijiu Cheng Jiandong Wu Regulation of Drought and Salt Tolerance by OsSKL2 and OsASR1 in Rice Rice Salt and drought tolerance Oryza sativa OsSKL2 Reactive oxygen species Protein interactions OsASR1 |
| title | Regulation of Drought and Salt Tolerance by OsSKL2 and OsASR1 in Rice |
| title_full | Regulation of Drought and Salt Tolerance by OsSKL2 and OsASR1 in Rice |
| title_fullStr | Regulation of Drought and Salt Tolerance by OsSKL2 and OsASR1 in Rice |
| title_full_unstemmed | Regulation of Drought and Salt Tolerance by OsSKL2 and OsASR1 in Rice |
| title_short | Regulation of Drought and Salt Tolerance by OsSKL2 and OsASR1 in Rice |
| title_sort | regulation of drought and salt tolerance by osskl2 and osasr1 in rice |
| topic | Salt and drought tolerance Oryza sativa OsSKL2 Reactive oxygen species Protein interactions OsASR1 |
| url | https://doi.org/10.1186/s12284-022-00592-2 |
| work_keys_str_mv | AT yinglijiang regulationofdroughtandsalttolerancebyosskl2andosasr1inrice AT xiaojianpeng regulationofdroughtandsalttolerancebyosskl2andosasr1inrice AT qinzhang regulationofdroughtandsalttolerancebyosskl2andosasr1inrice AT yuqingliu regulationofdroughtandsalttolerancebyosskl2andosasr1inrice AT aiqili regulationofdroughtandsalttolerancebyosskl2andosasr1inrice AT beijiucheng regulationofdroughtandsalttolerancebyosskl2andosasr1inrice AT jiandongwu regulationofdroughtandsalttolerancebyosskl2andosasr1inrice |