Potential pathways and genes expressed in Chrysanthemum in response to early fusarium oxysporum infection
Abstract Background Chrysanthemum Fusarium wilt is a common fungal disease caused by Fusarium oxysporum, which causes continuous cropping obstacles and huge losses to the chrysanthemum industry. The defense mechanism of chrysanthemum against F. oxysporum remains unclear, especially during the early...
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04331-7 |
| _version_ | 1797801534900142080 |
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| author | Weihao Miao Yanrong Yang Mengtong Wu Gan huang Lijiao Ge Ye Liu Zhiyong Guan Sumei Chen Weimin Fang Fadi Chen Shuang Zhao |
| author_facet | Weihao Miao Yanrong Yang Mengtong Wu Gan huang Lijiao Ge Ye Liu Zhiyong Guan Sumei Chen Weimin Fang Fadi Chen Shuang Zhao |
| author_sort | Weihao Miao |
| collection | DOAJ |
| description | Abstract Background Chrysanthemum Fusarium wilt is a common fungal disease caused by Fusarium oxysporum, which causes continuous cropping obstacles and huge losses to the chrysanthemum industry. The defense mechanism of chrysanthemum against F. oxysporum remains unclear, especially during the early stages of the disease. Therefore, in the present study, we analyzed chrysanthemum ‘Jinba’ samples inoculated with F. oxysporum at 0, 3, and 72 h using RNA-seq. Results The results revealed that 7985 differentially expressed genes (DEGs) were co-expressed at 3 and 72 h after F. oxysporum infection. We analyzed the identified DEGs using Kyoto Encyclopedia of Genes and Genomes and Gene Ontology. The DEGs were primarily enriched in “Plant pathogen interaction”, “MAPK signaling pathway”, “Starch and sucrose metabolism”, and “Biosynthesis of secondary metabolites”. Genes related to the synthesis of secondary metabolites were upregulated in chrysanthemum early during the inoculation period. Furthermore, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase enzymes were consistently produced to accumulate large amounts of phenolic compounds to resist F. oxysporum infection. Additionally, genes related to the proline metabolic pathway were upregulated, and proline levels accumulated within 72 h, regulating osmotic balance in chrysanthemum. Notably, the soluble sugar content in chrysanthemum decreased early during the inoculation period; we speculate that this is a self-protective mechanism of chrysanthemums for inhibiting fungal reproduction by reducing the sugar content in vivo. In the meantime, we screened for transcription factors that respond to F. oxysporum at an early stage and analyzed the relationship between WRKY and DEGs in the “Plant-pathogen interaction” pathway. We screened a key WRKY as a research target for subsequent experiments. Conclusion This study revealed the relevant physiological responses and gene expression changes in chrysanthemum in response to F. oxysporum infection, and provided a relevant candidate gene pool for subsequent studies on chrysanthemum Fusarium wilt. |
| first_indexed | 2024-03-13T04:51:56Z |
| format | Article |
| id | doaj.art-d82c010103784c0eabc05ce30b752d48 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-13T04:51:56Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj.art-d82c010103784c0eabc05ce30b752d482023-06-18T11:11:01ZengBMCBMC Plant Biology1471-22292023-06-0123111410.1186/s12870-023-04331-7Potential pathways and genes expressed in Chrysanthemum in response to early fusarium oxysporum infectionWeihao Miao0Yanrong Yang1Mengtong Wu2Gan huang3Lijiao Ge4Ye Liu5Zhiyong Guan6Sumei Chen7Weimin Fang8Fadi Chen9Shuang Zhao10College of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityCollege of Horticulture, Nanjing Agricultural UniversityAbstract Background Chrysanthemum Fusarium wilt is a common fungal disease caused by Fusarium oxysporum, which causes continuous cropping obstacles and huge losses to the chrysanthemum industry. The defense mechanism of chrysanthemum against F. oxysporum remains unclear, especially during the early stages of the disease. Therefore, in the present study, we analyzed chrysanthemum ‘Jinba’ samples inoculated with F. oxysporum at 0, 3, and 72 h using RNA-seq. Results The results revealed that 7985 differentially expressed genes (DEGs) were co-expressed at 3 and 72 h after F. oxysporum infection. We analyzed the identified DEGs using Kyoto Encyclopedia of Genes and Genomes and Gene Ontology. The DEGs were primarily enriched in “Plant pathogen interaction”, “MAPK signaling pathway”, “Starch and sucrose metabolism”, and “Biosynthesis of secondary metabolites”. Genes related to the synthesis of secondary metabolites were upregulated in chrysanthemum early during the inoculation period. Furthermore, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase enzymes were consistently produced to accumulate large amounts of phenolic compounds to resist F. oxysporum infection. Additionally, genes related to the proline metabolic pathway were upregulated, and proline levels accumulated within 72 h, regulating osmotic balance in chrysanthemum. Notably, the soluble sugar content in chrysanthemum decreased early during the inoculation period; we speculate that this is a self-protective mechanism of chrysanthemums for inhibiting fungal reproduction by reducing the sugar content in vivo. In the meantime, we screened for transcription factors that respond to F. oxysporum at an early stage and analyzed the relationship between WRKY and DEGs in the “Plant-pathogen interaction” pathway. We screened a key WRKY as a research target for subsequent experiments. Conclusion This study revealed the relevant physiological responses and gene expression changes in chrysanthemum in response to F. oxysporum infection, and provided a relevant candidate gene pool for subsequent studies on chrysanthemum Fusarium wilt.https://doi.org/10.1186/s12870-023-04331-7ChrysanthemumFusarium wiltFusarium oxysporumRNA-seqWRKY |
| spellingShingle | Weihao Miao Yanrong Yang Mengtong Wu Gan huang Lijiao Ge Ye Liu Zhiyong Guan Sumei Chen Weimin Fang Fadi Chen Shuang Zhao Potential pathways and genes expressed in Chrysanthemum in response to early fusarium oxysporum infection BMC Plant Biology Chrysanthemum Fusarium wilt Fusarium oxysporum RNA-seq WRKY |
| title | Potential pathways and genes expressed in Chrysanthemum in response to early fusarium oxysporum infection |
| title_full | Potential pathways and genes expressed in Chrysanthemum in response to early fusarium oxysporum infection |
| title_fullStr | Potential pathways and genes expressed in Chrysanthemum in response to early fusarium oxysporum infection |
| title_full_unstemmed | Potential pathways and genes expressed in Chrysanthemum in response to early fusarium oxysporum infection |
| title_short | Potential pathways and genes expressed in Chrysanthemum in response to early fusarium oxysporum infection |
| title_sort | potential pathways and genes expressed in chrysanthemum in response to early fusarium oxysporum infection |
| topic | Chrysanthemum Fusarium wilt Fusarium oxysporum RNA-seq WRKY |
| url | https://doi.org/10.1186/s12870-023-04331-7 |
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