Voluntary Exercise Improves Cardiac Function and Prevents Cardiac Remodeling in a Mouse Model of Dilated Cardiomyopathy
Objective: Despite the indubitable beneficial effect of exercise to prevent of cardiovascular diseases, there is still a lack of studies investigating the impact of exercise in non-ischemic dilated cardiomyopathy. Here, we investigated the impact of voluntary exercise on cardiac function in a mouse...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2017-11-01
|
| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fphys.2017.00899/full |
| _version_ | 1818484851839336448 |
|---|---|
| author | Robin Deloux Robin Deloux Damien Vitiello Damien Vitiello Damien Vitiello Nathalie Mougenot Philippe Noirez Philippe Noirez Zhenlin Li Mathias Mericskay Mathias Mericskay Arnaud Ferry Arnaud Ferry Onnik Agbulut |
| author_facet | Robin Deloux Robin Deloux Damien Vitiello Damien Vitiello Damien Vitiello Nathalie Mougenot Philippe Noirez Philippe Noirez Zhenlin Li Mathias Mericskay Mathias Mericskay Arnaud Ferry Arnaud Ferry Onnik Agbulut |
| author_sort | Robin Deloux |
| collection | DOAJ |
| description | Objective: Despite the indubitable beneficial effect of exercise to prevent of cardiovascular diseases, there is still a lack of studies investigating the impact of exercise in non-ischemic dilated cardiomyopathy. Here, we investigated the impact of voluntary exercise on cardiac function in a mouse model of non-ischemic dilated cardiomyopathy (αMHC-MerCreMer:Sf/Sf), induced by cardiac-specific inactivation of the Serum Response Factor.Materials and Methods: Seven days after tamoxifen injection, 20 αMHC-MerCreMer:Sf/Sf mice were assigned to sedentary (n = 8) and exercise (n = 12) groups. Seven additional αMHC-MerCreMer:Sf/Sf mice without tamoxifen injection were used as control. The exercise group performed 4 weeks of voluntary running on wheel (1.8 ± 0.12 km/day). Cardiac function, myocardial fibrosis, and mitochondrial energetic pathways were then blindly assessed.Results: Exercised mice exhibited a smaller decrease of left ventricular (LV) fractional shortening and ejection fraction compared to control mice. This was associated with a lower degree of LV remodeling in exercised mice, as shown by a lower LV end-systolic intrerventricular septal and posterior wall thickness decrease from baseline values compared to sedentary mice. Moreover, exercised mice displayed a reduced gene expression of atrial and brain natriuretic factors. These benefits were associated by a reduced level of myocardial fibrosis. In addition, exercised mice exhibited a higher mitochondrial aconitase, voltage-dependent anion-selective channel 1 and PPAR gamma coactivators-1 alpha proteins levels suggesting that the increase of mitochondrial biogenesis and/or metabolism slowed the progression of dilated cardiomyopathy in exercised animals.Conclusions: In conclusion, our results support the role of voluntary exercise to improve outcomes in non-ischemic dilated heart failure (HF) and also support its potential for a routine clinical use in the future. |
| first_indexed | 2024-12-10T16:00:21Z |
| format | Article |
| id | doaj.art-d82d82ce46514ef2be43079a5f65ba2f |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-10T16:00:21Z |
| publishDate | 2017-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-d82d82ce46514ef2be43079a5f65ba2f2022-12-22T01:42:28ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2017-11-01810.3389/fphys.2017.00899292266Voluntary Exercise Improves Cardiac Function and Prevents Cardiac Remodeling in a Mouse Model of Dilated CardiomyopathyRobin Deloux0Robin Deloux1Damien Vitiello2Damien Vitiello3Damien Vitiello4Nathalie Mougenot5Philippe Noirez6Philippe Noirez7Zhenlin Li8Mathias Mericskay9Mathias Mericskay10Arnaud Ferry11Arnaud Ferry12Onnik Agbulut13Sorbonne Universités, UPMC University Paris 06, Institut de Biologie Paris-Seine, UMR Centre National de la Recherche Scientifique 8256, Biological Adaptation and Aging, Paris, FranceUMR-S 1180, National Institute for Health and Medical Research, University Paris-Sud, Université Paris-Saclay, Châtenay-Malabry, FranceSorbonne Universités, UPMC University Paris 06, Institut de Biologie Paris-Seine, UMR Centre National de la Recherche Scientifique 8256, Biological Adaptation and Aging, Paris, FranceSorbonne Paris Cité, Université Paris Descartes, Paris, FranceInstitute for Research in Medicine and Epidemiology of Sport, EA7329, National Institute of Sport, Expertise and Performance, Université Paris Descartes, Paris, FranceSorbonne Universités, UPMC University Paris 06, UMS28, Plateforme d'Expérimentation Coeur, Muscles, Vaisseaux, Paris, FranceSorbonne Paris Cité, Université Paris Descartes, Paris, FranceInstitute for Research in Medicine and Epidemiology of Sport, EA7329, National Institute of Sport, Expertise and Performance, Université Paris Descartes, Paris, FranceSorbonne Universités, UPMC University Paris 06, Institut de Biologie Paris-Seine, UMR Centre National de la Recherche Scientifique 8256, Biological Adaptation and Aging, Paris, FranceSorbonne Universités, UPMC University Paris 06, Institut de Biologie Paris-Seine, UMR Centre National de la Recherche Scientifique 8256, Biological Adaptation and Aging, Paris, FranceUMR-S 1180, National Institute for Health and Medical Research, University Paris-Sud, Université Paris-Saclay, Châtenay-Malabry, FranceSorbonne Paris Cité, Université Paris Descartes, Paris, FranceSorbonne Universités, UPMC University Paris 06, Institut de Myologie, UMR-S 794, National Institute for Health and Medical Research, UMR Centre National De La Recherche Scientifique 7215, Paris, FranceSorbonne Universités, UPMC University Paris 06, Institut de Biologie Paris-Seine, UMR Centre National de la Recherche Scientifique 8256, Biological Adaptation and Aging, Paris, FranceObjective: Despite the indubitable beneficial effect of exercise to prevent of cardiovascular diseases, there is still a lack of studies investigating the impact of exercise in non-ischemic dilated cardiomyopathy. Here, we investigated the impact of voluntary exercise on cardiac function in a mouse model of non-ischemic dilated cardiomyopathy (αMHC-MerCreMer:Sf/Sf), induced by cardiac-specific inactivation of the Serum Response Factor.Materials and Methods: Seven days after tamoxifen injection, 20 αMHC-MerCreMer:Sf/Sf mice were assigned to sedentary (n = 8) and exercise (n = 12) groups. Seven additional αMHC-MerCreMer:Sf/Sf mice without tamoxifen injection were used as control. The exercise group performed 4 weeks of voluntary running on wheel (1.8 ± 0.12 km/day). Cardiac function, myocardial fibrosis, and mitochondrial energetic pathways were then blindly assessed.Results: Exercised mice exhibited a smaller decrease of left ventricular (LV) fractional shortening and ejection fraction compared to control mice. This was associated with a lower degree of LV remodeling in exercised mice, as shown by a lower LV end-systolic intrerventricular septal and posterior wall thickness decrease from baseline values compared to sedentary mice. Moreover, exercised mice displayed a reduced gene expression of atrial and brain natriuretic factors. These benefits were associated by a reduced level of myocardial fibrosis. In addition, exercised mice exhibited a higher mitochondrial aconitase, voltage-dependent anion-selective channel 1 and PPAR gamma coactivators-1 alpha proteins levels suggesting that the increase of mitochondrial biogenesis and/or metabolism slowed the progression of dilated cardiomyopathy in exercised animals.Conclusions: In conclusion, our results support the role of voluntary exercise to improve outcomes in non-ischemic dilated heart failure (HF) and also support its potential for a routine clinical use in the future.http://journal.frontiersin.org/article/10.3389/fphys.2017.00899/fullnon-ischemic cardiac diseasedilated cardiomyopathynon-forced exercisewheel exercise |
| spellingShingle | Robin Deloux Robin Deloux Damien Vitiello Damien Vitiello Damien Vitiello Nathalie Mougenot Philippe Noirez Philippe Noirez Zhenlin Li Mathias Mericskay Mathias Mericskay Arnaud Ferry Arnaud Ferry Onnik Agbulut Voluntary Exercise Improves Cardiac Function and Prevents Cardiac Remodeling in a Mouse Model of Dilated Cardiomyopathy Frontiers in Physiology non-ischemic cardiac disease dilated cardiomyopathy non-forced exercise wheel exercise |
| title | Voluntary Exercise Improves Cardiac Function and Prevents Cardiac Remodeling in a Mouse Model of Dilated Cardiomyopathy |
| title_full | Voluntary Exercise Improves Cardiac Function and Prevents Cardiac Remodeling in a Mouse Model of Dilated Cardiomyopathy |
| title_fullStr | Voluntary Exercise Improves Cardiac Function and Prevents Cardiac Remodeling in a Mouse Model of Dilated Cardiomyopathy |
| title_full_unstemmed | Voluntary Exercise Improves Cardiac Function and Prevents Cardiac Remodeling in a Mouse Model of Dilated Cardiomyopathy |
| title_short | Voluntary Exercise Improves Cardiac Function and Prevents Cardiac Remodeling in a Mouse Model of Dilated Cardiomyopathy |
| title_sort | voluntary exercise improves cardiac function and prevents cardiac remodeling in a mouse model of dilated cardiomyopathy |
| topic | non-ischemic cardiac disease dilated cardiomyopathy non-forced exercise wheel exercise |
| url | http://journal.frontiersin.org/article/10.3389/fphys.2017.00899/full |
| work_keys_str_mv | AT robindeloux voluntaryexerciseimprovescardiacfunctionandpreventscardiacremodelinginamousemodelofdilatedcardiomyopathy AT robindeloux voluntaryexerciseimprovescardiacfunctionandpreventscardiacremodelinginamousemodelofdilatedcardiomyopathy AT damienvitiello voluntaryexerciseimprovescardiacfunctionandpreventscardiacremodelinginamousemodelofdilatedcardiomyopathy AT damienvitiello voluntaryexerciseimprovescardiacfunctionandpreventscardiacremodelinginamousemodelofdilatedcardiomyopathy AT damienvitiello voluntaryexerciseimprovescardiacfunctionandpreventscardiacremodelinginamousemodelofdilatedcardiomyopathy AT nathaliemougenot voluntaryexerciseimprovescardiacfunctionandpreventscardiacremodelinginamousemodelofdilatedcardiomyopathy AT philippenoirez voluntaryexerciseimprovescardiacfunctionandpreventscardiacremodelinginamousemodelofdilatedcardiomyopathy AT philippenoirez voluntaryexerciseimprovescardiacfunctionandpreventscardiacremodelinginamousemodelofdilatedcardiomyopathy AT zhenlinli voluntaryexerciseimprovescardiacfunctionandpreventscardiacremodelinginamousemodelofdilatedcardiomyopathy AT mathiasmericskay voluntaryexerciseimprovescardiacfunctionandpreventscardiacremodelinginamousemodelofdilatedcardiomyopathy AT mathiasmericskay voluntaryexerciseimprovescardiacfunctionandpreventscardiacremodelinginamousemodelofdilatedcardiomyopathy AT arnaudferry voluntaryexerciseimprovescardiacfunctionandpreventscardiacremodelinginamousemodelofdilatedcardiomyopathy AT arnaudferry voluntaryexerciseimprovescardiacfunctionandpreventscardiacremodelinginamousemodelofdilatedcardiomyopathy AT onnikagbulut voluntaryexerciseimprovescardiacfunctionandpreventscardiacremodelinginamousemodelofdilatedcardiomyopathy |