Genome-wide identification and expression analyses of CYP450 genes in sweet potato (Ipomoea batatas L.)
Abstract Background Cytochrome P450 monooxygenases (CYP450s) play a crucial role in various biochemical reactions involved in the synthesis of antioxidants, pigments, structural polymers, and defense-related compounds in plants. As sweet potato (Ipomoea batatas L.) holds significant economic importa...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-024-09965-x |
| _version_ | 1797356022471327744 |
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| author | Xiongjian Lin Binquan Tang Zhenqin Li Lei Shi Hongbo Zhu |
| author_facet | Xiongjian Lin Binquan Tang Zhenqin Li Lei Shi Hongbo Zhu |
| author_sort | Xiongjian Lin |
| collection | DOAJ |
| description | Abstract Background Cytochrome P450 monooxygenases (CYP450s) play a crucial role in various biochemical reactions involved in the synthesis of antioxidants, pigments, structural polymers, and defense-related compounds in plants. As sweet potato (Ipomoea batatas L.) holds significant economic importance, a comprehensive analysis of CYP450 genes in this plant species can offer valuable insights into the evolutionary relationships and functional characteristics of these genes. Results In this study, we successfully identified and categorized 95 CYP450 genes from the sweet potato genome into 5 families and 31 subfamilies. The predicted subcellular localization results indicate that CYP450s are distributed in the cell membrane system. The promoter region of the IbCYP450 genes contains various cis-acting elements related to plant hormones and stress responses. In addition, ten conserved motifs (Motif1-Motif10) have been identified in the IbCYP450 family proteins, with 5 genes lacking introns and only one exon. We observed extensive duplication events within the CYP450 gene family, which may account for its expansion. The gene duplication analysis results showed the presence of 15 pairs of genes with tandem repeats. Interaction network analysis reveals that IbCYP450 families can interact with multiple target genes and there are protein-protein interactions within the family. Transcription factor interaction analysis suggests that IbCYP450 families interact with multiple transcription factors. Furthermore, gene expression analysis revealed tissue-specific expression patterns of CYP450 genes in sweet potatoes, as well as their response to abiotic stress and plant hormones. Notably, quantitative real-time polymerase chain reaction (qRT‒PCR) analysis indicated the involvement of CYP450 genes in the defense response against nonbiological stresses in sweet potatoes. Conclusions These findings provide a foundation for further investigations aiming to elucidate the biological functions of CYP450 genes in sweet potatoes. |
| first_indexed | 2024-03-08T14:19:36Z |
| format | Article |
| id | doaj.art-d8309af3a25046e8ba95212833e67947 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-08T14:19:36Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-d8309af3a25046e8ba95212833e679472024-01-14T12:13:00ZengBMCBMC Genomics1471-21642024-01-0125112410.1186/s12864-024-09965-xGenome-wide identification and expression analyses of CYP450 genes in sweet potato (Ipomoea batatas L.)Xiongjian Lin0Binquan Tang1Zhenqin Li2Lei Shi3Hongbo Zhu4College of Coastal Agricultural Sciences, Guangdong Ocean UniversityCollege of Coastal Agricultural Sciences, Guangdong Ocean UniversityCollege of Coastal Agricultural Sciences, Guangdong Ocean UniversityCollege of Coastal Agricultural Sciences, Guangdong Ocean UniversityCollege of Coastal Agricultural Sciences, Guangdong Ocean UniversityAbstract Background Cytochrome P450 monooxygenases (CYP450s) play a crucial role in various biochemical reactions involved in the synthesis of antioxidants, pigments, structural polymers, and defense-related compounds in plants. As sweet potato (Ipomoea batatas L.) holds significant economic importance, a comprehensive analysis of CYP450 genes in this plant species can offer valuable insights into the evolutionary relationships and functional characteristics of these genes. Results In this study, we successfully identified and categorized 95 CYP450 genes from the sweet potato genome into 5 families and 31 subfamilies. The predicted subcellular localization results indicate that CYP450s are distributed in the cell membrane system. The promoter region of the IbCYP450 genes contains various cis-acting elements related to plant hormones and stress responses. In addition, ten conserved motifs (Motif1-Motif10) have been identified in the IbCYP450 family proteins, with 5 genes lacking introns and only one exon. We observed extensive duplication events within the CYP450 gene family, which may account for its expansion. The gene duplication analysis results showed the presence of 15 pairs of genes with tandem repeats. Interaction network analysis reveals that IbCYP450 families can interact with multiple target genes and there are protein-protein interactions within the family. Transcription factor interaction analysis suggests that IbCYP450 families interact with multiple transcription factors. Furthermore, gene expression analysis revealed tissue-specific expression patterns of CYP450 genes in sweet potatoes, as well as their response to abiotic stress and plant hormones. Notably, quantitative real-time polymerase chain reaction (qRT‒PCR) analysis indicated the involvement of CYP450 genes in the defense response against nonbiological stresses in sweet potatoes. Conclusions These findings provide a foundation for further investigations aiming to elucidate the biological functions of CYP450 genes in sweet potatoes.https://doi.org/10.1186/s12864-024-09965-xCYP450Gene familySweet potatoExpression analysis |
| spellingShingle | Xiongjian Lin Binquan Tang Zhenqin Li Lei Shi Hongbo Zhu Genome-wide identification and expression analyses of CYP450 genes in sweet potato (Ipomoea batatas L.) BMC Genomics CYP450 Gene family Sweet potato Expression analysis |
| title | Genome-wide identification and expression analyses of CYP450 genes in sweet potato (Ipomoea batatas L.) |
| title_full | Genome-wide identification and expression analyses of CYP450 genes in sweet potato (Ipomoea batatas L.) |
| title_fullStr | Genome-wide identification and expression analyses of CYP450 genes in sweet potato (Ipomoea batatas L.) |
| title_full_unstemmed | Genome-wide identification and expression analyses of CYP450 genes in sweet potato (Ipomoea batatas L.) |
| title_short | Genome-wide identification and expression analyses of CYP450 genes in sweet potato (Ipomoea batatas L.) |
| title_sort | genome wide identification and expression analyses of cyp450 genes in sweet potato ipomoea batatas l |
| topic | CYP450 Gene family Sweet potato Expression analysis |
| url | https://doi.org/10.1186/s12864-024-09965-x |
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