SLC17A9-PTHLH-EMT axis promotes proliferation and invasion of clear renal cell carcinoma
Summary: SLC17A9 is a vesicular ATP transport protein that plays an important role in determining cell functions and the onset and progression of different diseases. In this study, SLC17A9 was initially identified as a potential diagnostic and prognostic risk biomarker for clear cell renal cell carc...
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| Format: | Article |
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Elsevier
2023-01-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222020375 |
| _version_ | 1797946192550690816 |
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| author | Weiquan Li Ning Xu Xiangui Meng Hongwei Yuan Tiexi Yu Qi Miao Hongmei Yang Bo Hai Wen Xiao Xiaoping Zhang |
| author_facet | Weiquan Li Ning Xu Xiangui Meng Hongwei Yuan Tiexi Yu Qi Miao Hongmei Yang Bo Hai Wen Xiao Xiaoping Zhang |
| author_sort | Weiquan Li |
| collection | DOAJ |
| description | Summary: SLC17A9 is a vesicular ATP transport protein that plays an important role in determining cell functions and the onset and progression of different diseases. In this study, SLC17A9 was initially identified as a potential diagnostic and prognostic risk biomarker for clear cell renal cell carcinoma (ccRCC). Then, the aberrant expression levels of SLC17A9 were confirmed in both the cell lines and clinical tissues. Mechanistically, SLC17A9 could upregulate the expression of PTHLH, thus promoting epithelial-mesenchymal transition (EMT) in ccRCC. Functionally, SLC17A9 knockdown inhibited the proliferation, migration, and invasion activity of renal cancer cells, whereas its overexpression led to stronger cell viability and more malignant phenotype in vitro. The overexpression of SLC17A9 in vivo could significantly contribute to the growth of tumors. Finally, we found that SLC17A9 might be related to the drug resistance of vorinostat. Cumulatively, this study demonstrated that the SLC17A9-PTHLH-EMT axis could promote the progression of ccRCC. |
| first_indexed | 2024-04-10T21:06:59Z |
| format | Article |
| id | doaj.art-d83594f282444c6cae56427b1ddc2d62 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-10T21:06:59Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-d83594f282444c6cae56427b1ddc2d622023-01-22T04:40:49ZengElsevieriScience2589-00422023-01-01261105764SLC17A9-PTHLH-EMT axis promotes proliferation and invasion of clear renal cell carcinomaWeiquan Li0Ning Xu1Xiangui Meng2Hongwei Yuan3Tiexi Yu4Qi Miao5Hongmei Yang6Bo Hai7Wen Xiao8Xiaoping Zhang9Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen 518000, ChinaDepartment of Pathogenic Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaDepartment of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen 518000, ChinaDepartment of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen 518000, ChinaDepartment of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen 518000, ChinaDepartment of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen 518000, ChinaDepartment of Pathogenic Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaDepartment of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen 518000, China; Corresponding authorDepartment of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen 518000, China; Corresponding authorDepartment of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen 518000, China; Corresponding authorSummary: SLC17A9 is a vesicular ATP transport protein that plays an important role in determining cell functions and the onset and progression of different diseases. In this study, SLC17A9 was initially identified as a potential diagnostic and prognostic risk biomarker for clear cell renal cell carcinoma (ccRCC). Then, the aberrant expression levels of SLC17A9 were confirmed in both the cell lines and clinical tissues. Mechanistically, SLC17A9 could upregulate the expression of PTHLH, thus promoting epithelial-mesenchymal transition (EMT) in ccRCC. Functionally, SLC17A9 knockdown inhibited the proliferation, migration, and invasion activity of renal cancer cells, whereas its overexpression led to stronger cell viability and more malignant phenotype in vitro. The overexpression of SLC17A9 in vivo could significantly contribute to the growth of tumors. Finally, we found that SLC17A9 might be related to the drug resistance of vorinostat. Cumulatively, this study demonstrated that the SLC17A9-PTHLH-EMT axis could promote the progression of ccRCC.http://www.sciencedirect.com/science/article/pii/S2589004222020375Biological sciencesCell biologyCancer |
| spellingShingle | Weiquan Li Ning Xu Xiangui Meng Hongwei Yuan Tiexi Yu Qi Miao Hongmei Yang Bo Hai Wen Xiao Xiaoping Zhang SLC17A9-PTHLH-EMT axis promotes proliferation and invasion of clear renal cell carcinoma iScience Biological sciences Cell biology Cancer |
| title | SLC17A9-PTHLH-EMT axis promotes proliferation and invasion of clear renal cell carcinoma |
| title_full | SLC17A9-PTHLH-EMT axis promotes proliferation and invasion of clear renal cell carcinoma |
| title_fullStr | SLC17A9-PTHLH-EMT axis promotes proliferation and invasion of clear renal cell carcinoma |
| title_full_unstemmed | SLC17A9-PTHLH-EMT axis promotes proliferation and invasion of clear renal cell carcinoma |
| title_short | SLC17A9-PTHLH-EMT axis promotes proliferation and invasion of clear renal cell carcinoma |
| title_sort | slc17a9 pthlh emt axis promotes proliferation and invasion of clear renal cell carcinoma |
| topic | Biological sciences Cell biology Cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222020375 |
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