The <i>MKK2a</i> Gene Involved in the MAPK Signaling Cascades Enhances <i>Populus</i> Salt Tolerance
Mitogen-activated protein kinase (MAPK) cascades are highly conserved signal transduction modules, which transmit environmental signals in plant cells through stepwise phosphorylation and play indispensable roles in a wide range of physiological and biochemical processes. Here, we isolated and chara...
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/17/10185 |
| _version_ | 1797494894644690944 |
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| author | Jiali Wang Zimou Sun Caihui Chen Meng Xu |
| author_facet | Jiali Wang Zimou Sun Caihui Chen Meng Xu |
| author_sort | Jiali Wang |
| collection | DOAJ |
| description | Mitogen-activated protein kinase (MAPK) cascades are highly conserved signal transduction modules, which transmit environmental signals in plant cells through stepwise phosphorylation and play indispensable roles in a wide range of physiological and biochemical processes. Here, we isolated and characterized a gene encoding MKK2 protein from poplar through the rapid amplification of cDNA ends (RACE). The full-length <i>PeMKK2a</i> gene was 1571 bp, including a 1068 bp open reading frame (ORF) encoding 355 amino acids, and the putative PeMKK2a protein belongs to the PKc_like (protein kinase domain) family (70–336 amino acids) in the PKc_MAPKK_plant subfamily and contains 62 sites of possible phosphorylation and two conserved domains, DLK and S/T-xxxxx-S/T. Detailed information about its gene structure, sequence similarities, subcellular localization, and transcript profiles under salt-stress conditions was revealed. Transgenic poplar lines overexpressing <i>PeMKK2a</i> exhibited higher activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) than non-transgenic poplar under salt stress conditions. These results will provide insight into the roles of MAPK signaling cascades in poplar response to salt stress. |
| first_indexed | 2024-03-10T01:40:48Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:40:48Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d8386c9b4efd4080aaabf699ec898e512023-11-23T13:24:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123171018510.3390/ijms231710185The <i>MKK2a</i> Gene Involved in the MAPK Signaling Cascades Enhances <i>Populus</i> Salt ToleranceJiali Wang0Zimou Sun1Caihui Chen2Meng Xu3Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, ChinaMitogen-activated protein kinase (MAPK) cascades are highly conserved signal transduction modules, which transmit environmental signals in plant cells through stepwise phosphorylation and play indispensable roles in a wide range of physiological and biochemical processes. Here, we isolated and characterized a gene encoding MKK2 protein from poplar through the rapid amplification of cDNA ends (RACE). The full-length <i>PeMKK2a</i> gene was 1571 bp, including a 1068 bp open reading frame (ORF) encoding 355 amino acids, and the putative PeMKK2a protein belongs to the PKc_like (protein kinase domain) family (70–336 amino acids) in the PKc_MAPKK_plant subfamily and contains 62 sites of possible phosphorylation and two conserved domains, DLK and S/T-xxxxx-S/T. Detailed information about its gene structure, sequence similarities, subcellular localization, and transcript profiles under salt-stress conditions was revealed. Transgenic poplar lines overexpressing <i>PeMKK2a</i> exhibited higher activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) than non-transgenic poplar under salt stress conditions. These results will provide insight into the roles of MAPK signaling cascades in poplar response to salt stress.https://www.mdpi.com/1422-0067/23/17/10185MAPK signaling cascades<i>PeMKK2a</i> genesalt stressoverexpression<i>Populus</i> |
| spellingShingle | Jiali Wang Zimou Sun Caihui Chen Meng Xu The <i>MKK2a</i> Gene Involved in the MAPK Signaling Cascades Enhances <i>Populus</i> Salt Tolerance International Journal of Molecular Sciences MAPK signaling cascades <i>PeMKK2a</i> gene salt stress overexpression <i>Populus</i> |
| title | The <i>MKK2a</i> Gene Involved in the MAPK Signaling Cascades Enhances <i>Populus</i> Salt Tolerance |
| title_full | The <i>MKK2a</i> Gene Involved in the MAPK Signaling Cascades Enhances <i>Populus</i> Salt Tolerance |
| title_fullStr | The <i>MKK2a</i> Gene Involved in the MAPK Signaling Cascades Enhances <i>Populus</i> Salt Tolerance |
| title_full_unstemmed | The <i>MKK2a</i> Gene Involved in the MAPK Signaling Cascades Enhances <i>Populus</i> Salt Tolerance |
| title_short | The <i>MKK2a</i> Gene Involved in the MAPK Signaling Cascades Enhances <i>Populus</i> Salt Tolerance |
| title_sort | i mkk2a i gene involved in the mapk signaling cascades enhances i populus i salt tolerance |
| topic | MAPK signaling cascades <i>PeMKK2a</i> gene salt stress overexpression <i>Populus</i> |
| url | https://www.mdpi.com/1422-0067/23/17/10185 |
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