Potential Screening of Bacteriocinogenic-Lactic Acid Bacteria from Mangrove Sediment of Logending Beach for Fisheries Product Preservation
The meat and fisheries products have high nutritional content which is highly ideal for bacterial growth. Lactic Acid Bacteria (LAB) have several potential advantages as a bio-preservative agent in the food industry because they produce antimicrobial substances against pathogenic bacteria e.g. bacte...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
2021-04-01
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| Series: | Journal of Tropical Biodiversity and Biotechnology |
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| Online Access: | https://jurnal.ugm.ac.id/jtbb/article/view/61927 |
| _version_ | 1818718400716734464 |
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| author | Dyah Fitri Kusharyati Taruna Dwi Satwika Afifah Mariana Anwar Rovik |
| author_facet | Dyah Fitri Kusharyati Taruna Dwi Satwika Afifah Mariana Anwar Rovik |
| author_sort | Dyah Fitri Kusharyati |
| collection | DOAJ |
| description | The meat and fisheries products have high nutritional content which is highly ideal for bacterial growth. Lactic Acid Bacteria (LAB) have several potential advantages as a bio-preservative agent in the food industry because they produce antimicrobial substances against pathogenic bacteria e.g. bacteriocin. Our previous study has succeeded in isolating and characterizing LAB from the mangrove sediments of Logending Beach, Kebumen. This present study aimed to determine the activity of bacteriocinogenic-LAB against food-borne pathogens and their potential for fisheries product preservation. The study consisted of five serial stages, as follows: screening of LAB isolates, cell-free supernatant production and its inhibition activity, extraction of partially purified bacteriocin, bacteriocin confirmation against proteolytic enzymes, and in-vitro test of partially-purified bacteriocin against Listeria monocytogenes, Shigella flexneri, and Salmonella typhi. A total of 25 out of 99 isolates were able to grow on MRSA+1% CaCO3 medium. Initial screening showed that the cell-free supernatant of 14 LAB isolates was able to inhibit the growth of S. thypi, S. flexneri, and L. monocytogenes. There was an increased inhibitory activity of partially purified bacteriocin when compared with the cell-free supernatant which was statistically different (p<0.01). It indicated that the purification was successfully performed. Bacteriocin expressed a lower inhibition against S. typhi than L. monocytogenes and S. flexneri. The ANOVA test showed that each indicator pathogenic-bacterium expresses a very significant sensitivity to the partially purified bacteriocin. |
| first_indexed | 2024-12-17T19:50:27Z |
| format | Article |
| id | doaj.art-d8396aebc62e46a2afff275401c18915 |
| institution | Directory Open Access Journal |
| issn | 2540-9573 2540-9581 |
| language | English |
| last_indexed | 2024-12-17T19:50:27Z |
| publishDate | 2021-04-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Journal of Tropical Biodiversity and Biotechnology |
| spelling | doaj.art-d8396aebc62e46a2afff275401c189152022-12-21T21:34:44ZengUniversitas Gadjah MadaJournal of Tropical Biodiversity and Biotechnology2540-95732540-95812021-04-016110.22146/jtbb.6192729575Potential Screening of Bacteriocinogenic-Lactic Acid Bacteria from Mangrove Sediment of Logending Beach for Fisheries Product PreservationDyah Fitri Kusharyati0Taruna Dwi Satwika1Afifah Mariana2Anwar Rovik3Department of Microbiology, Faculty of Biology, Universitas Jenderal SoedirmanDepartment of Microbiology, Faculty of Biology, Universitas Jenderal SoedirmanDepartment of Microbiology, Faculty of Biology, Universitas Jenderal SoedirmanCentre of Tropical Medicine. Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, IndonesiaThe meat and fisheries products have high nutritional content which is highly ideal for bacterial growth. Lactic Acid Bacteria (LAB) have several potential advantages as a bio-preservative agent in the food industry because they produce antimicrobial substances against pathogenic bacteria e.g. bacteriocin. Our previous study has succeeded in isolating and characterizing LAB from the mangrove sediments of Logending Beach, Kebumen. This present study aimed to determine the activity of bacteriocinogenic-LAB against food-borne pathogens and their potential for fisheries product preservation. The study consisted of five serial stages, as follows: screening of LAB isolates, cell-free supernatant production and its inhibition activity, extraction of partially purified bacteriocin, bacteriocin confirmation against proteolytic enzymes, and in-vitro test of partially-purified bacteriocin against Listeria monocytogenes, Shigella flexneri, and Salmonella typhi. A total of 25 out of 99 isolates were able to grow on MRSA+1% CaCO3 medium. Initial screening showed that the cell-free supernatant of 14 LAB isolates was able to inhibit the growth of S. thypi, S. flexneri, and L. monocytogenes. There was an increased inhibitory activity of partially purified bacteriocin when compared with the cell-free supernatant which was statistically different (p<0.01). It indicated that the purification was successfully performed. Bacteriocin expressed a lower inhibition against S. typhi than L. monocytogenes and S. flexneri. The ANOVA test showed that each indicator pathogenic-bacterium expresses a very significant sensitivity to the partially purified bacteriocin.https://jurnal.ugm.ac.id/jtbb/article/view/61927bacteriocinfisheries productlabmangrove sedimentsfood-borne pathogens |
| spellingShingle | Dyah Fitri Kusharyati Taruna Dwi Satwika Afifah Mariana Anwar Rovik Potential Screening of Bacteriocinogenic-Lactic Acid Bacteria from Mangrove Sediment of Logending Beach for Fisheries Product Preservation Journal of Tropical Biodiversity and Biotechnology bacteriocin fisheries product lab mangrove sediments food-borne pathogens |
| title | Potential Screening of Bacteriocinogenic-Lactic Acid Bacteria from Mangrove Sediment of Logending Beach for Fisheries Product Preservation |
| title_full | Potential Screening of Bacteriocinogenic-Lactic Acid Bacteria from Mangrove Sediment of Logending Beach for Fisheries Product Preservation |
| title_fullStr | Potential Screening of Bacteriocinogenic-Lactic Acid Bacteria from Mangrove Sediment of Logending Beach for Fisheries Product Preservation |
| title_full_unstemmed | Potential Screening of Bacteriocinogenic-Lactic Acid Bacteria from Mangrove Sediment of Logending Beach for Fisheries Product Preservation |
| title_short | Potential Screening of Bacteriocinogenic-Lactic Acid Bacteria from Mangrove Sediment of Logending Beach for Fisheries Product Preservation |
| title_sort | potential screening of bacteriocinogenic lactic acid bacteria from mangrove sediment of logending beach for fisheries product preservation |
| topic | bacteriocin fisheries product lab mangrove sediments food-borne pathogens |
| url | https://jurnal.ugm.ac.id/jtbb/article/view/61927 |
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