Glutamate Receptor GRIA3—Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer
Previously, we identified the transcription factor CUX1 as an important modulator of invasion and resistance to apoptosis. Expression profiles suggested that CUX1 regulates a complex transcriptional program mediating tumor progression. We aimed to identify functionally relevant targets of CUX1 by us...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2010-08-01
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| Series: | Neoplasia: An International Journal for Oncology Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1476558610800673 |
| _version_ | 1818345808576118784 |
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| author | Stefanie Ripka Jan Riedel Albrecht Neesse Heidi Griesmann Malte Buchholz Volker Ellenrieder Franz Moeller Peter Bartht Thomas M. Gress Patrick Michl |
| author_facet | Stefanie Ripka Jan Riedel Albrecht Neesse Heidi Griesmann Malte Buchholz Volker Ellenrieder Franz Moeller Peter Bartht Thomas M. Gress Patrick Michl |
| author_sort | Stefanie Ripka |
| collection | DOAJ |
| description | Previously, we identified the transcription factor CUX1 as an important modulator of invasion and resistance to apoptosis. Expression profiles suggested that CUX1 regulates a complex transcriptional program mediating tumor progression. We aimed to identify functionally relevant targets of CUX1 by using RNA interference (RNAi)-based loss-of-function screens. Therefore, we generated an RNAi library containing putative transcriptional targets of CUX1 identified by microarrays and performed cell viability screens. Using this approach, several CUX1 targets with effect on tumor cell viability were identified, including the glutamate receptor GRIA3, which was validated in detail for its effects on proliferation, apoptosis, and cell migration using RNAi knock-down and overexpression strategies in vitro, as well as xenograft models in vivo. The expression of GRIA3 was evaluated in human pancreatic cancer tissues. We found that knock-down of GRIA3 significantly reduced proliferation and migration and enhanced apoptosis. In contrast, overexpression of GRIA3 significantly reduced apoptosis and enhanced both proliferation and tumor cell migration. GRIA3 could be confirmed as a downstream effector of CUX1 and was expressed in pancreatic cancer tissues. In vivo, GRIA3 significantly enhanced the growth of subcutaneous xenografts. Inhibitors of glutamate receptors such as GYKI52466 and SYM2206 significantly decreased survival of pancreatic cancer cells, suggesting the presence of glutamate signaling in pancreatic cancer. In conclusion, GRIA3 plays a role as a mediator of tumor progression in pancreatic cancer downstream CUX1. To our knowledge, this is the first report to identify a glutamate receptor as a modulator of tumor progression in a solid cancer outside the brain. |
| first_indexed | 2024-12-13T17:08:15Z |
| format | Article |
| id | doaj.art-d83ab5a5fccb4793ad449d836f0104fc |
| institution | Directory Open Access Journal |
| issn | 1476-5586 1522-8002 |
| language | English |
| last_indexed | 2024-12-13T17:08:15Z |
| publishDate | 2010-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neoplasia: An International Journal for Oncology Research |
| spelling | doaj.art-d83ab5a5fccb4793ad449d836f0104fc2022-12-21T23:37:37ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022010-08-0112865966710.1593/neo.10486Glutamate Receptor GRIA3—Target of CUX1 and Mediator of Tumor Progression in Pancreatic CancerStefanie Ripka0Jan Riedel1Albrecht Neesse2Heidi Griesmann3Malte Buchholz4Volker Ellenrieder5Franz Moeller6Peter Bartht7Thomas M. Gress8Patrick Michl9Department of Gastroenterology, University Hospital, Philipps University, Marburg, GermanyDepartment of Gastroenterology, University Hospital, Philipps University, Marburg, GermanyDepartment of Gastroenterology, University Hospital, Philipps University, Marburg, GermanyDepartment of Gastroenterology, University Hospital, Philipps University, Marburg, GermanyDepartment of Gastroenterology, University Hospital, Philipps University, Marburg, GermanyDepartment of Gastroenterology, University Hospital, Philipps University, Marburg, GermanyDepartment of Gastroenterology, University Hospital, Philipps University, Marburg, GermanyDepartment of Pathology, University Hospital, Philipps University, Marburg, GermanyDepartment of Gastroenterology, University Hospital, Philipps University, Marburg, GermanyDepartment of Gastroenterology, University Hospital, Philipps University, Marburg, GermanyPreviously, we identified the transcription factor CUX1 as an important modulator of invasion and resistance to apoptosis. Expression profiles suggested that CUX1 regulates a complex transcriptional program mediating tumor progression. We aimed to identify functionally relevant targets of CUX1 by using RNA interference (RNAi)-based loss-of-function screens. Therefore, we generated an RNAi library containing putative transcriptional targets of CUX1 identified by microarrays and performed cell viability screens. Using this approach, several CUX1 targets with effect on tumor cell viability were identified, including the glutamate receptor GRIA3, which was validated in detail for its effects on proliferation, apoptosis, and cell migration using RNAi knock-down and overexpression strategies in vitro, as well as xenograft models in vivo. The expression of GRIA3 was evaluated in human pancreatic cancer tissues. We found that knock-down of GRIA3 significantly reduced proliferation and migration and enhanced apoptosis. In contrast, overexpression of GRIA3 significantly reduced apoptosis and enhanced both proliferation and tumor cell migration. GRIA3 could be confirmed as a downstream effector of CUX1 and was expressed in pancreatic cancer tissues. In vivo, GRIA3 significantly enhanced the growth of subcutaneous xenografts. Inhibitors of glutamate receptors such as GYKI52466 and SYM2206 significantly decreased survival of pancreatic cancer cells, suggesting the presence of glutamate signaling in pancreatic cancer. In conclusion, GRIA3 plays a role as a mediator of tumor progression in pancreatic cancer downstream CUX1. To our knowledge, this is the first report to identify a glutamate receptor as a modulator of tumor progression in a solid cancer outside the brain.http://www.sciencedirect.com/science/article/pii/S1476558610800673 |
| spellingShingle | Stefanie Ripka Jan Riedel Albrecht Neesse Heidi Griesmann Malte Buchholz Volker Ellenrieder Franz Moeller Peter Bartht Thomas M. Gress Patrick Michl Glutamate Receptor GRIA3—Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer Neoplasia: An International Journal for Oncology Research |
| title | Glutamate Receptor GRIA3—Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer |
| title_full | Glutamate Receptor GRIA3—Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer |
| title_fullStr | Glutamate Receptor GRIA3—Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer |
| title_full_unstemmed | Glutamate Receptor GRIA3—Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer |
| title_short | Glutamate Receptor GRIA3—Target of CUX1 and Mediator of Tumor Progression in Pancreatic Cancer |
| title_sort | glutamate receptor gria3 target of cux1 and mediator of tumor progression in pancreatic cancer |
| url | http://www.sciencedirect.com/science/article/pii/S1476558610800673 |
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