Ultrastructural Characterization of Human Gingival Fibroblasts in 3D Culture
Cell spheroids are applied in various fields of research, such as the fabrication of three-dimensional artificial tissues in vitro, disease modeling, stem cell research, regenerative therapy, and biotechnology. A preclinical 3D culture model of primary human gingival fibroblasts free of external fac...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/22/3647 |
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| author | Sandra Liliana Alfonso García Laura Marcela Mira Uribe Susana Castaño López Monica Tatiana Parada-Sanchez David Arboleda-Toro |
| author_facet | Sandra Liliana Alfonso García Laura Marcela Mira Uribe Susana Castaño López Monica Tatiana Parada-Sanchez David Arboleda-Toro |
| author_sort | Sandra Liliana Alfonso García |
| collection | DOAJ |
| description | Cell spheroids are applied in various fields of research, such as the fabrication of three-dimensional artificial tissues in vitro, disease modeling, stem cell research, regenerative therapy, and biotechnology. A preclinical 3D culture model of primary human gingival fibroblasts free of external factors and/or chemical inducers is presented herein. The ultrastructure of the spheroids was characterized to establish a cellular model for the study of periodontal tissue regeneration. The liquid overlay technique was used with agarose to generate spheroids. Fibroblasts in 2D culture and cell spheroids were characterized by immunofluorescence, and cell spheroids were characterized by optical and scanning electron microscopy, energy-dispersive X-ray spectroscopy, backscattered electrons, and Fourier transform infrared spectroscopy. Ostegenic related genes were analyzed by RT-qPCR. Gingival fibroblasts formed spheroids spontaneously and showed amorphous calcium phosphate nanoparticle deposits on their surface. The results suggest that human gingival fibroblasts have an intrinsic potential to generate a mineralized niche in 3D culture. |
| first_indexed | 2024-03-09T18:25:49Z |
| format | Article |
| id | doaj.art-d83da6fd807f497f89efb7396d95aff0 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T18:25:49Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-d83da6fd807f497f89efb7396d95aff02023-11-24T07:58:47ZengMDPI AGCells2073-44092022-11-011122364710.3390/cells11223647Ultrastructural Characterization of Human Gingival Fibroblasts in 3D CultureSandra Liliana Alfonso García0Laura Marcela Mira Uribe1Susana Castaño López2Monica Tatiana Parada-Sanchez3David Arboleda-Toro4Department of Oral Health, Faculty of Dentistry, Universidad Nacional de Colombia, Bogotá 111321, ColombiaSchool Bioscience, Faculty of Science, Universidad Nacional de Colombia, Medellín 050034, ColombiaSchool Bioscience, Faculty of Science, Universidad Nacional de Colombia, Medellín 050034, ColombiaDepartment of Integrated Basic Studies, Faculty of Dentistry, Universidad de Antioquia, Medellín 050010, ColombiaDepartment of Integrated Basic Studies, Faculty of Dentistry, Universidad de Antioquia, Medellín 050010, ColombiaCell spheroids are applied in various fields of research, such as the fabrication of three-dimensional artificial tissues in vitro, disease modeling, stem cell research, regenerative therapy, and biotechnology. A preclinical 3D culture model of primary human gingival fibroblasts free of external factors and/or chemical inducers is presented herein. The ultrastructure of the spheroids was characterized to establish a cellular model for the study of periodontal tissue regeneration. The liquid overlay technique was used with agarose to generate spheroids. Fibroblasts in 2D culture and cell spheroids were characterized by immunofluorescence, and cell spheroids were characterized by optical and scanning electron microscopy, energy-dispersive X-ray spectroscopy, backscattered electrons, and Fourier transform infrared spectroscopy. Ostegenic related genes were analyzed by RT-qPCR. Gingival fibroblasts formed spheroids spontaneously and showed amorphous calcium phosphate nanoparticle deposits on their surface. The results suggest that human gingival fibroblasts have an intrinsic potential to generate a mineralized niche in 3D culture.https://www.mdpi.com/2073-4409/11/22/36473D culturegingival fibroblastsamorphous calcium phosphateperiodontics |
| spellingShingle | Sandra Liliana Alfonso García Laura Marcela Mira Uribe Susana Castaño López Monica Tatiana Parada-Sanchez David Arboleda-Toro Ultrastructural Characterization of Human Gingival Fibroblasts in 3D Culture Cells 3D culture gingival fibroblasts amorphous calcium phosphate periodontics |
| title | Ultrastructural Characterization of Human Gingival Fibroblasts in 3D Culture |
| title_full | Ultrastructural Characterization of Human Gingival Fibroblasts in 3D Culture |
| title_fullStr | Ultrastructural Characterization of Human Gingival Fibroblasts in 3D Culture |
| title_full_unstemmed | Ultrastructural Characterization of Human Gingival Fibroblasts in 3D Culture |
| title_short | Ultrastructural Characterization of Human Gingival Fibroblasts in 3D Culture |
| title_sort | ultrastructural characterization of human gingival fibroblasts in 3d culture |
| topic | 3D culture gingival fibroblasts amorphous calcium phosphate periodontics |
| url | https://www.mdpi.com/2073-4409/11/22/3647 |
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