Semi-quantitative analysis with 99mTc-Besilesomab in musculoskeletal system infections
Objectives: To evaluate the usefulness of 99mTc-Besilesomab to diagnose infectious processes by using monoclonal antibodies BW 250/183 in a semi-quantitative analysis, and to determine the effect on diagnostic capacity of different thresholds for the difference between counts in early versus delayed...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Bone Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352187223000566 |
| _version_ | 1827591153876205568 |
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| author | Patricia Guardia-Jimena María Dolores Martínez-Valle Torres Raquel Arenas Aguaza Amanda Rocío González Ramírez Diego Becerra-García Damián Guirado |
| author_facet | Patricia Guardia-Jimena María Dolores Martínez-Valle Torres Raquel Arenas Aguaza Amanda Rocío González Ramírez Diego Becerra-García Damián Guirado |
| author_sort | Patricia Guardia-Jimena |
| collection | DOAJ |
| description | Objectives: To evaluate the usefulness of 99mTc-Besilesomab to diagnose infectious processes by using monoclonal antibodies BW 250/183 in a semi-quantitative analysis, and to determine the effect on diagnostic capacity of different thresholds for the difference between counts in early versus delayed images. Methods: The study included 77 patients with suspected osteomyelitis who underwent scintigraphy with 99mTc-Besilesomab. After confirming the absence of human anti-mouse antibodies in all patients, early and delayed static images were acquired at 4 and 24 h post-injection, respectively. Visual and semi-quantitative analyses were conducted of regions of interest (ROIs) in areas suspected of infection on early and delayed images. Findings were considered positive when the ratio between counts in delayed and early images exceeded a given threshold after correction for decay. The definitive diagnosis was obtained by clinical follow-up, microbiological culture, or response to medical and/or surgical treatment. Results: The optimal threshold was 1.02 (i.e., positive result = count increase of >2 % in delayed image), obtaining a sensitivity of 0.864, specificity of 0.858, positive predictive value of 0.708, negative predictive value of 0.940, and accuracy of 0.860. Application of the usual threshold of 1.10 (10 %) reduced the sensitivity to 0.734. Conclusion: Semi-quantitative analysis of studies with 99mTc-Besilesomab is a useful technique for the diagnosis of musculoskeletal system infections and contributes to the definitive diagnosis when visual assessments are doubtful or non-conclusive. |
| first_indexed | 2024-03-09T01:27:48Z |
| format | Article |
| id | doaj.art-d83e21dd55204a98a9d579f489e910a0 |
| institution | Directory Open Access Journal |
| issn | 2352-1872 |
| language | English |
| last_indexed | 2024-03-09T01:27:48Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Bone Reports |
| spelling | doaj.art-d83e21dd55204a98a9d579f489e910a02023-12-10T06:16:19ZengElsevierBone Reports2352-18722023-12-0119101708Semi-quantitative analysis with 99mTc-Besilesomab in musculoskeletal system infectionsPatricia Guardia-Jimena0María Dolores Martínez-Valle Torres1Raquel Arenas Aguaza2Amanda Rocío González Ramírez3Diego Becerra-García4Damián Guirado5Nuclear Medicine Service, San Cecilio Clinical University Hospital, Granada, SpainNuclear Medicine Service, San Cecilio Clinical University Hospital, Granada, SpainNuclear Medicine Service, San Cecilio Clinical University Hospital, Granada, SpainMethodological and Statistical Support Unit of the Fundación Pública Andaluza para la Investigación Biosanitaria Andalucía Oriental (FIBAO), Clinical University Hospital, Granada, Spain; Instituto de Investigación Biosanitaria (ibs.GRANADA), Radiophysics Unit, San Cecilio Clinical University Hospital, Granada, SpainNuclear Medicine Service, San Cecilio Clinical University Hospital, Granada, SpainInstituto de Investigación Biosanitaria (ibs.GRANADA), Radiophysics Unit, San Cecilio Clinical University Hospital, Granada, Spain; CIBER de Epidemiología y Salud Pública (CIBERESP), Granada, Spain; Corresponding author at: Instituto de Investigación Biosanitaria (ibs.GRANADA), Radiophysics Unit, San Cecilio Clinical University Hospital, Granada, Spain.Objectives: To evaluate the usefulness of 99mTc-Besilesomab to diagnose infectious processes by using monoclonal antibodies BW 250/183 in a semi-quantitative analysis, and to determine the effect on diagnostic capacity of different thresholds for the difference between counts in early versus delayed images. Methods: The study included 77 patients with suspected osteomyelitis who underwent scintigraphy with 99mTc-Besilesomab. After confirming the absence of human anti-mouse antibodies in all patients, early and delayed static images were acquired at 4 and 24 h post-injection, respectively. Visual and semi-quantitative analyses were conducted of regions of interest (ROIs) in areas suspected of infection on early and delayed images. Findings were considered positive when the ratio between counts in delayed and early images exceeded a given threshold after correction for decay. The definitive diagnosis was obtained by clinical follow-up, microbiological culture, or response to medical and/or surgical treatment. Results: The optimal threshold was 1.02 (i.e., positive result = count increase of >2 % in delayed image), obtaining a sensitivity of 0.864, specificity of 0.858, positive predictive value of 0.708, negative predictive value of 0.940, and accuracy of 0.860. Application of the usual threshold of 1.10 (10 %) reduced the sensitivity to 0.734. Conclusion: Semi-quantitative analysis of studies with 99mTc-Besilesomab is a useful technique for the diagnosis of musculoskeletal system infections and contributes to the definitive diagnosis when visual assessments are doubtful or non-conclusive.http://www.sciencedirect.com/science/article/pii/S2352187223000566Monoclonal antibodies99mTc-BesilesomabInfectionSemi-quantitative analysisMusculoskeletal system |
| spellingShingle | Patricia Guardia-Jimena María Dolores Martínez-Valle Torres Raquel Arenas Aguaza Amanda Rocío González Ramírez Diego Becerra-García Damián Guirado Semi-quantitative analysis with 99mTc-Besilesomab in musculoskeletal system infections Bone Reports Monoclonal antibodies 99mTc-Besilesomab Infection Semi-quantitative analysis Musculoskeletal system |
| title | Semi-quantitative analysis with 99mTc-Besilesomab in musculoskeletal system infections |
| title_full | Semi-quantitative analysis with 99mTc-Besilesomab in musculoskeletal system infections |
| title_fullStr | Semi-quantitative analysis with 99mTc-Besilesomab in musculoskeletal system infections |
| title_full_unstemmed | Semi-quantitative analysis with 99mTc-Besilesomab in musculoskeletal system infections |
| title_short | Semi-quantitative analysis with 99mTc-Besilesomab in musculoskeletal system infections |
| title_sort | semi quantitative analysis with 99mtc besilesomab in musculoskeletal system infections |
| topic | Monoclonal antibodies 99mTc-Besilesomab Infection Semi-quantitative analysis Musculoskeletal system |
| url | http://www.sciencedirect.com/science/article/pii/S2352187223000566 |
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