Sustainable carbon sources for microbial organic acid production with filamentous fungi

Abstract Background The organic acid producer Aspergillus oryzae and Rhizopus delemar are able to convert several alternative carbon sources to malic and fumaric acid. Thus, carbohydrate hydrolysates from lignocellulose separation are likely suitable as substrate for organic acid production with the...

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Main Authors: Stefan Dörsam, Jana Fesseler, Olga Gorte, Thomas Hahn, Susanne Zibek, Christoph Syldatk, Katrin Ochsenreither
Format: Article
Language:English
Published: BMC 2017-10-01
Series:Biotechnology for Biofuels
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13068-017-0930-x
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author Stefan Dörsam
Jana Fesseler
Olga Gorte
Thomas Hahn
Susanne Zibek
Christoph Syldatk
Katrin Ochsenreither
author_facet Stefan Dörsam
Jana Fesseler
Olga Gorte
Thomas Hahn
Susanne Zibek
Christoph Syldatk
Katrin Ochsenreither
author_sort Stefan Dörsam
collection DOAJ
description Abstract Background The organic acid producer Aspergillus oryzae and Rhizopus delemar are able to convert several alternative carbon sources to malic and fumaric acid. Thus, carbohydrate hydrolysates from lignocellulose separation are likely suitable as substrate for organic acid production with these fungi. Results Before lignocellulose hydrolysate fractions were tested as substrates, experiments with several mono- and disaccharides, possibly present in pretreated biomass, were conducted for their suitability for malic acid production with A. oryzae. This includes levoglucosan, glucose, galactose, mannose, arabinose, xylose, ribose, and cellobiose as well as cheap and easy available sugars, e.g., fructose and maltose. A. oryzae is able to convert every sugar investigated to malate, albeit with different yields. Based on the promising results from the pure sugar conversion experiments, fractions of the organosolv process from beechwood (Fagus sylvatica) and Miscanthus giganteus were further analyzed as carbon source for cultivation and fermentation with A. oryzae for malic acid and R. delemar for fumaric acid production. The highest malic acid concentration of 37.9 ± 2.6 g/L could be reached using beechwood cellulose fraction as carbon source in bioreactor fermentation with A. oryzae and 16.2 ± 0.2 g/L fumaric acid with R. delemar. Conclusions We showed in this study that the range of convertible sugars for A. oryzae is even higher than known before. We approved the suitability of fiber/cellulose hydrolysate obtained from the organosolv process as carbon source for A. oryzae in shake flasks as well as in a small-scale bioreactor. The more challenging hemicellulose fraction of F. sylvatica was also positively evaluated for malic acid production with A. oryzae.
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spelling doaj.art-d83eaa7c54314149adaf1b989624fb0f2022-12-22T00:13:59ZengBMCBiotechnology for Biofuels1754-68342017-10-0110111210.1186/s13068-017-0930-xSustainable carbon sources for microbial organic acid production with filamentous fungiStefan Dörsam0Jana Fesseler1Olga Gorte2Thomas Hahn3Susanne Zibek4Christoph Syldatk5Katrin Ochsenreither6Technical Biology, Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology (KIT)Technical Biology, Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology (KIT)Technical Biology, Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology (KIT)Industrial Biotechnology, Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB)Industrial Biotechnology, Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB)Technical Biology, Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology (KIT)Technical Biology, Institute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology (KIT)Abstract Background The organic acid producer Aspergillus oryzae and Rhizopus delemar are able to convert several alternative carbon sources to malic and fumaric acid. Thus, carbohydrate hydrolysates from lignocellulose separation are likely suitable as substrate for organic acid production with these fungi. Results Before lignocellulose hydrolysate fractions were tested as substrates, experiments with several mono- and disaccharides, possibly present in pretreated biomass, were conducted for their suitability for malic acid production with A. oryzae. This includes levoglucosan, glucose, galactose, mannose, arabinose, xylose, ribose, and cellobiose as well as cheap and easy available sugars, e.g., fructose and maltose. A. oryzae is able to convert every sugar investigated to malate, albeit with different yields. Based on the promising results from the pure sugar conversion experiments, fractions of the organosolv process from beechwood (Fagus sylvatica) and Miscanthus giganteus were further analyzed as carbon source for cultivation and fermentation with A. oryzae for malic acid and R. delemar for fumaric acid production. The highest malic acid concentration of 37.9 ± 2.6 g/L could be reached using beechwood cellulose fraction as carbon source in bioreactor fermentation with A. oryzae and 16.2 ± 0.2 g/L fumaric acid with R. delemar. Conclusions We showed in this study that the range of convertible sugars for A. oryzae is even higher than known before. We approved the suitability of fiber/cellulose hydrolysate obtained from the organosolv process as carbon source for A. oryzae in shake flasks as well as in a small-scale bioreactor. The more challenging hemicellulose fraction of F. sylvatica was also positively evaluated for malic acid production with A. oryzae.http://link.springer.com/article/10.1186/s13068-017-0930-xAspergillus oryzaeRhizopus delemarMalic acidMalateFermentationOrganic acid
spellingShingle Stefan Dörsam
Jana Fesseler
Olga Gorte
Thomas Hahn
Susanne Zibek
Christoph Syldatk
Katrin Ochsenreither
Sustainable carbon sources for microbial organic acid production with filamentous fungi
Biotechnology for Biofuels
Aspergillus oryzae
Rhizopus delemar
Malic acid
Malate
Fermentation
Organic acid
title Sustainable carbon sources for microbial organic acid production with filamentous fungi
title_full Sustainable carbon sources for microbial organic acid production with filamentous fungi
title_fullStr Sustainable carbon sources for microbial organic acid production with filamentous fungi
title_full_unstemmed Sustainable carbon sources for microbial organic acid production with filamentous fungi
title_short Sustainable carbon sources for microbial organic acid production with filamentous fungi
title_sort sustainable carbon sources for microbial organic acid production with filamentous fungi
topic Aspergillus oryzae
Rhizopus delemar
Malic acid
Malate
Fermentation
Organic acid
url http://link.springer.com/article/10.1186/s13068-017-0930-x
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AT thomashahn sustainablecarbonsourcesformicrobialorganicacidproductionwithfilamentousfungi
AT susannezibek sustainablecarbonsourcesformicrobialorganicacidproductionwithfilamentousfungi
AT christophsyldatk sustainablecarbonsourcesformicrobialorganicacidproductionwithfilamentousfungi
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