Characterization of the Human Eccrine Sweat Proteome—A Focus on the Biological Variability of Individual Sweat Protein Profiles
The potential of eccrine sweat as a bio-fluid of interest for diagnosis and personalized therapy has not yet been fully evaluated, due to the lack of in-depth sweat characterization studies. Thanks to recent developments in omics, together with the availability of accredited sweat collection methods...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/19/10871 |
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| author | Bastien Burat Audrey Reynaerts Dominique Baiwir Maximilien Fléron Gauthier Eppe Teresinha Leal Gabriel Mazzucchelli |
| author_facet | Bastien Burat Audrey Reynaerts Dominique Baiwir Maximilien Fléron Gauthier Eppe Teresinha Leal Gabriel Mazzucchelli |
| author_sort | Bastien Burat |
| collection | DOAJ |
| description | The potential of eccrine sweat as a bio-fluid of interest for diagnosis and personalized therapy has not yet been fully evaluated, due to the lack of in-depth sweat characterization studies. Thanks to recent developments in omics, together with the availability of accredited sweat collection methods, the analysis of human sweat may now be envisioned as a standardized, non-invasive test for individualized monitoring and personalized medicine. Here, we characterized individual sweat samples, collected from 28 healthy adult volunteers under the most standardized sampling methodology, by applying optimized shotgun proteomics. The thorough characterization of the sweat proteome allowed the identification of 983 unique proteins from which 344 were identified across all samples. Annotation-wise, the study of the sweat proteome unveiled the over-representation of newly addressed actin dynamics, oxidative stress and proteasome-related functions, in addition to well-described proteolysis and anti-microbial immunity. The sweat proteome composition correlated with the inter-individual variability of sweat secretion parameters. In addition, both gender-exclusive proteins and gender-specific protein abundances were highlighted, despite the high similarity between human female and male sweat proteomes. In conclusion, standardized sample collection coupled with optimized shotgun proteomics significantly improved the depth of sweat proteome coverage, far beyond previous similar studies. The identified proteins were involved in many diverse biological processes and molecular functions, indicating the potential of this bio-fluid as a valuable biological matrix for further studies. Addressing sweat variability, our results prove the proteomic profiling of sweat to be a promising bio-fluid analysis for individualized, non-invasive monitoring and personalized medicine. |
| first_indexed | 2024-03-10T06:59:19Z |
| format | Article |
| id | doaj.art-d8412a6aeaf840758888153dc79d5134 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:59:19Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d8412a6aeaf840758888153dc79d51342023-11-22T16:16:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-10-0122191087110.3390/ijms221910871Characterization of the Human Eccrine Sweat Proteome—A Focus on the Biological Variability of Individual Sweat Protein ProfilesBastien Burat0Audrey Reynaerts1Dominique Baiwir2Maximilien Fléron3Gauthier Eppe4Teresinha Leal5Gabriel Mazzucchelli6Mass Spectrometry Laboratory, MolSys Research Unit, Liège Université, B-4000 Liège, BelgiumLouvain Center for Toxicology and Applied Pharmacology (LTAP), Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, B-1200 Brussels, BelgiumGIGA Proteomics Facility, Liège Université, B-4000 Liège, BelgiumGIGA Proteomics Facility, Liège Université, B-4000 Liège, BelgiumMass Spectrometry Laboratory, MolSys Research Unit, Liège Université, B-4000 Liège, BelgiumLouvain Center for Toxicology and Applied Pharmacology (LTAP), Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, B-1200 Brussels, BelgiumMass Spectrometry Laboratory, MolSys Research Unit, Liège Université, B-4000 Liège, BelgiumThe potential of eccrine sweat as a bio-fluid of interest for diagnosis and personalized therapy has not yet been fully evaluated, due to the lack of in-depth sweat characterization studies. Thanks to recent developments in omics, together with the availability of accredited sweat collection methods, the analysis of human sweat may now be envisioned as a standardized, non-invasive test for individualized monitoring and personalized medicine. Here, we characterized individual sweat samples, collected from 28 healthy adult volunteers under the most standardized sampling methodology, by applying optimized shotgun proteomics. The thorough characterization of the sweat proteome allowed the identification of 983 unique proteins from which 344 were identified across all samples. Annotation-wise, the study of the sweat proteome unveiled the over-representation of newly addressed actin dynamics, oxidative stress and proteasome-related functions, in addition to well-described proteolysis and anti-microbial immunity. The sweat proteome composition correlated with the inter-individual variability of sweat secretion parameters. In addition, both gender-exclusive proteins and gender-specific protein abundances were highlighted, despite the high similarity between human female and male sweat proteomes. In conclusion, standardized sample collection coupled with optimized shotgun proteomics significantly improved the depth of sweat proteome coverage, far beyond previous similar studies. The identified proteins were involved in many diverse biological processes and molecular functions, indicating the potential of this bio-fluid as a valuable biological matrix for further studies. Addressing sweat variability, our results prove the proteomic profiling of sweat to be a promising bio-fluid analysis for individualized, non-invasive monitoring and personalized medicine.https://www.mdpi.com/1422-0067/22/19/10871human eccrine sweatshotgun proteomicsinter-individual variability |
| spellingShingle | Bastien Burat Audrey Reynaerts Dominique Baiwir Maximilien Fléron Gauthier Eppe Teresinha Leal Gabriel Mazzucchelli Characterization of the Human Eccrine Sweat Proteome—A Focus on the Biological Variability of Individual Sweat Protein Profiles International Journal of Molecular Sciences human eccrine sweat shotgun proteomics inter-individual variability |
| title | Characterization of the Human Eccrine Sweat Proteome—A Focus on the Biological Variability of Individual Sweat Protein Profiles |
| title_full | Characterization of the Human Eccrine Sweat Proteome—A Focus on the Biological Variability of Individual Sweat Protein Profiles |
| title_fullStr | Characterization of the Human Eccrine Sweat Proteome—A Focus on the Biological Variability of Individual Sweat Protein Profiles |
| title_full_unstemmed | Characterization of the Human Eccrine Sweat Proteome—A Focus on the Biological Variability of Individual Sweat Protein Profiles |
| title_short | Characterization of the Human Eccrine Sweat Proteome—A Focus on the Biological Variability of Individual Sweat Protein Profiles |
| title_sort | characterization of the human eccrine sweat proteome a focus on the biological variability of individual sweat protein profiles |
| topic | human eccrine sweat shotgun proteomics inter-individual variability |
| url | https://www.mdpi.com/1422-0067/22/19/10871 |
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