Analysis of Volatile Markers and Their Biotransformation in Raw Chicken during <i>Staphylococcus aureus</i> Early Contamination
To address the potential risks to food safety, headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS) and headspace gas chromatography–ion mobility spectrometry (HS-GC-IMS) were used to analyze the volatile organic compounds (VOCs) generated from chil...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/14/2782 |
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| author | Yin Wang Xian Wang Yuanyuan Huang Tianli Yue Wei Cao |
| author_facet | Yin Wang Xian Wang Yuanyuan Huang Tianli Yue Wei Cao |
| author_sort | Yin Wang |
| collection | DOAJ |
| description | To address the potential risks to food safety, headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS) and headspace gas chromatography–ion mobility spectrometry (HS-GC-IMS) were used to analyze the volatile organic compounds (VOCs) generated from chilled chicken contaminated with <i>Staphylococcus aureus</i> during early storage. Together with the KEGG database, we analyzed differential metabolites and their possible biotransformation pathways. Orthogonal partial least squares discriminant analysis (OPLS-DA) was applied to characterize VOCs and identify biomarkers associated with the early stage of chicken meat contamination with <i>S. aureus</i>. The results showed 2,6,10,15-tetramethylheptadecane, ethyl acetate, hexanal, 2-methylbutanal, butan-2-one, 3-hydroxy-2-butanone, 3-methylbutanal, and cyclohexanone as characteristic biomarkers, and 1-octen-3-ol, tetradecane, 2-hexanol, 3-methyl-1-butanol, and ethyl 2-methylpropanoate as potential characteristic biomarkers. This provides a theoretical basis for the study of biomarkers of <i>Staphylococcus aureus</i> in poultry meat. |
| first_indexed | 2024-03-11T01:05:00Z |
| format | Article |
| id | doaj.art-d84464a4e1894f968f31eb8791e3683a |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T01:05:00Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj.art-d84464a4e1894f968f31eb8791e3683a2023-11-18T19:21:24ZengMDPI AGFoods2304-81582023-07-011214278210.3390/foods12142782Analysis of Volatile Markers and Their Biotransformation in Raw Chicken during <i>Staphylococcus aureus</i> Early ContaminationYin Wang0Xian Wang1Yuanyuan Huang2Tianli Yue3Wei Cao4Department of Food Science, College of Food Science and Technology, Northwest University, Xi’an 710069, ChinaDepartment of Food Science, College of Food Science and Technology, Northwest University, Xi’an 710069, ChinaDepartment of Food Science, College of Food Science and Technology, Northwest University, Xi’an 710069, ChinaDepartment of Food Science, College of Food Science and Technology, Northwest University, Xi’an 710069, ChinaDepartment of Food Science, College of Food Science and Technology, Northwest University, Xi’an 710069, ChinaTo address the potential risks to food safety, headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS) and headspace gas chromatography–ion mobility spectrometry (HS-GC-IMS) were used to analyze the volatile organic compounds (VOCs) generated from chilled chicken contaminated with <i>Staphylococcus aureus</i> during early storage. Together with the KEGG database, we analyzed differential metabolites and their possible biotransformation pathways. Orthogonal partial least squares discriminant analysis (OPLS-DA) was applied to characterize VOCs and identify biomarkers associated with the early stage of chicken meat contamination with <i>S. aureus</i>. The results showed 2,6,10,15-tetramethylheptadecane, ethyl acetate, hexanal, 2-methylbutanal, butan-2-one, 3-hydroxy-2-butanone, 3-methylbutanal, and cyclohexanone as characteristic biomarkers, and 1-octen-3-ol, tetradecane, 2-hexanol, 3-methyl-1-butanol, and ethyl 2-methylpropanoate as potential characteristic biomarkers. This provides a theoretical basis for the study of biomarkers of <i>Staphylococcus aureus</i> in poultry meat.https://www.mdpi.com/2304-8158/12/14/2782VOCs<i>Staphylococcus aureus</i>HS-GC-IMSHS-SPME-GC-MSchicken |
| spellingShingle | Yin Wang Xian Wang Yuanyuan Huang Tianli Yue Wei Cao Analysis of Volatile Markers and Their Biotransformation in Raw Chicken during <i>Staphylococcus aureus</i> Early Contamination Foods VOCs <i>Staphylococcus aureus</i> HS-GC-IMS HS-SPME-GC-MS chicken |
| title | Analysis of Volatile Markers and Their Biotransformation in Raw Chicken during <i>Staphylococcus aureus</i> Early Contamination |
| title_full | Analysis of Volatile Markers and Their Biotransformation in Raw Chicken during <i>Staphylococcus aureus</i> Early Contamination |
| title_fullStr | Analysis of Volatile Markers and Their Biotransformation in Raw Chicken during <i>Staphylococcus aureus</i> Early Contamination |
| title_full_unstemmed | Analysis of Volatile Markers and Their Biotransformation in Raw Chicken during <i>Staphylococcus aureus</i> Early Contamination |
| title_short | Analysis of Volatile Markers and Their Biotransformation in Raw Chicken during <i>Staphylococcus aureus</i> Early Contamination |
| title_sort | analysis of volatile markers and their biotransformation in raw chicken during i staphylococcus aureus i early contamination |
| topic | VOCs <i>Staphylococcus aureus</i> HS-GC-IMS HS-SPME-GC-MS chicken |
| url | https://www.mdpi.com/2304-8158/12/14/2782 |
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