Aurora-B Promotes Osteosarcoma Cell Growth and Metastasis Through Activation of the NPM1/ERK/NF-κβ/MMPs Axis
Honghai Song,1,2,* Yang Zhou,1,* Aifen Peng,3 Jiaming Liu,1,2,4 Xin Wu,1 Wenzhao Chen,1 Zhili Liu1,2,5 1Department of Orthopedic Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, People’s Republic of China; 2Institute of Spinal and Spinal Cord Diseases, Nancha...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-06-01
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| Series: | Cancer Management and Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/aurora-b-promotes-osteosarcoma-cell-growth-and-metastasis-through-acti-peer-reviewed-article-CMAR |
| _version_ | 1818336743316783104 |
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| author | Song H Zhou Y Peng A Liu J Wu X Chen W Liu Z |
| author_facet | Song H Zhou Y Peng A Liu J Wu X Chen W Liu Z |
| author_sort | Song H |
| collection | DOAJ |
| description | Honghai Song,1,2,* Yang Zhou,1,* Aifen Peng,3 Jiaming Liu,1,2,4 Xin Wu,1 Wenzhao Chen,1 Zhili Liu1,2,5 1Department of Orthopedic Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, People’s Republic of China; 2Institute of Spinal and Spinal Cord Diseases, Nanchang University, Nanchang 330031, People’s Republic of China; 3College of Humanities, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, People’s Republic of China; 4Jiangxi Institute of Respiratory Disease, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China; 5Division of Science and Technology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhili LiuDepartment of Orthopedics, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of ChinaTel/ Fax +86-79188693201Email zgm7977@163.comPurpose: Osteosarcoma (OS) is the most common primary malignant tumor of the bone in young adolescents and children. We explored the underlying mechanism of Aurora-B in promoting OS cell proliferation and metastasis.Patient and Methods: Bioinformatics was employed to predict the substrate of Aurora-B. IHC and Western blot were used to confirm the correlation between Aurora-B and NPM1. ERK/NF-κβ pathway-related proteins were detected by Western blot and immunofluorescence (IF). CCK8, wound healing, transwell, and Tunel assays were used to identify the cell proliferation, migration and apoptosis potential. Spontaneous metastasis xenografts were established to confirm the role of Aurora-B and NPM1.Results: Aurora-B promotes NPM1 phosphorylation on Ser125. The phosphorylation of NPM1Ser125 induced by Aurora-B activates the ERK/NF-κβ signaling. Further study revealed that Aurora-B promotes proliferation, migration and inhibits apoptosis via phosphorylating NPM1 in vitro and in vivo.Conclusion: Aurora-B promotes OS malignancy via phosphorylating NPM1Ser125 and activating ERK/NF-κβ signaling.Keywords: Aurora-B, osteosarcoma, NPM1, ERK, NF-κβ |
| first_indexed | 2024-12-13T14:44:10Z |
| format | Article |
| id | doaj.art-d84531dbdd164fc2bf294d7ebab4f260 |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-13T14:44:10Z |
| publishDate | 2020-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-d84531dbdd164fc2bf294d7ebab4f2602022-12-21T23:41:31ZengDove Medical PressCancer Management and Research1179-13222020-06-01Volume 124817482754762Aurora-B Promotes Osteosarcoma Cell Growth and Metastasis Through Activation of the NPM1/ERK/NF-κβ/MMPs AxisSong HZhou YPeng ALiu JWu XChen WLiu ZHonghai Song,1,2,* Yang Zhou,1,* Aifen Peng,3 Jiaming Liu,1,2,4 Xin Wu,1 Wenzhao Chen,1 Zhili Liu1,2,5 1Department of Orthopedic Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330006, People’s Republic of China; 2Institute of Spinal and Spinal Cord Diseases, Nanchang University, Nanchang 330031, People’s Republic of China; 3College of Humanities, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330004, People’s Republic of China; 4Jiangxi Institute of Respiratory Disease, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China; 5Division of Science and Technology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhili LiuDepartment of Orthopedics, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of ChinaTel/ Fax +86-79188693201Email zgm7977@163.comPurpose: Osteosarcoma (OS) is the most common primary malignant tumor of the bone in young adolescents and children. We explored the underlying mechanism of Aurora-B in promoting OS cell proliferation and metastasis.Patient and Methods: Bioinformatics was employed to predict the substrate of Aurora-B. IHC and Western blot were used to confirm the correlation between Aurora-B and NPM1. ERK/NF-κβ pathway-related proteins were detected by Western blot and immunofluorescence (IF). CCK8, wound healing, transwell, and Tunel assays were used to identify the cell proliferation, migration and apoptosis potential. Spontaneous metastasis xenografts were established to confirm the role of Aurora-B and NPM1.Results: Aurora-B promotes NPM1 phosphorylation on Ser125. The phosphorylation of NPM1Ser125 induced by Aurora-B activates the ERK/NF-κβ signaling. Further study revealed that Aurora-B promotes proliferation, migration and inhibits apoptosis via phosphorylating NPM1 in vitro and in vivo.Conclusion: Aurora-B promotes OS malignancy via phosphorylating NPM1Ser125 and activating ERK/NF-κβ signaling.Keywords: Aurora-B, osteosarcoma, NPM1, ERK, NF-κβhttps://www.dovepress.com/aurora-b-promotes-osteosarcoma-cell-growth-and-metastasis-through-acti-peer-reviewed-article-CMARaurora-bosteosarcomanpm1erknf-κβ |
| spellingShingle | Song H Zhou Y Peng A Liu J Wu X Chen W Liu Z Aurora-B Promotes Osteosarcoma Cell Growth and Metastasis Through Activation of the NPM1/ERK/NF-κβ/MMPs Axis Cancer Management and Research aurora-b osteosarcoma npm1 erk nf-κβ |
| title | Aurora-B Promotes Osteosarcoma Cell Growth and Metastasis Through Activation of the NPM1/ERK/NF-κβ/MMPs Axis |
| title_full | Aurora-B Promotes Osteosarcoma Cell Growth and Metastasis Through Activation of the NPM1/ERK/NF-κβ/MMPs Axis |
| title_fullStr | Aurora-B Promotes Osteosarcoma Cell Growth and Metastasis Through Activation of the NPM1/ERK/NF-κβ/MMPs Axis |
| title_full_unstemmed | Aurora-B Promotes Osteosarcoma Cell Growth and Metastasis Through Activation of the NPM1/ERK/NF-κβ/MMPs Axis |
| title_short | Aurora-B Promotes Osteosarcoma Cell Growth and Metastasis Through Activation of the NPM1/ERK/NF-κβ/MMPs Axis |
| title_sort | aurora b promotes osteosarcoma cell growth and metastasis through activation of the npm1 erk nf kappa beta mmps axis |
| topic | aurora-b osteosarcoma npm1 erk nf-κβ |
| url | https://www.dovepress.com/aurora-b-promotes-osteosarcoma-cell-growth-and-metastasis-through-acti-peer-reviewed-article-CMAR |
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