The potential of exopolysaccharide-producing bacteria from rhizosphere of rubber plants for improving soil aggregate
This study aimed to examine the effect of bacteria found in the rhizosphere of rubber plants in producing exopolysaccharides to improve aggregate stability of sandy soils. Samples of soil have been taken in rhizosphere of rubber plants in West Kalimantan. Serial soil samples were diluted and culture...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2018-04-01
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| Series: | Journal of Degraded and Mining Lands Management |
| Subjects: | |
| Online Access: | http://jdmlm.ub.ac.id/index.php/jdmlm/article/view/427 |
| _version_ | 1818584967461994496 |
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| author | Nasrul Harahap Dwi Andreas Santosa Nuni Gofar |
| author_facet | Nasrul Harahap Dwi Andreas Santosa Nuni Gofar |
| author_sort | Nasrul Harahap |
| collection | DOAJ |
| description | This study aimed to examine the effect of bacteria found in the rhizosphere of rubber plants in producing exopolysaccharides to improve aggregate stability of sandy soils. Samples of soil have been taken in rhizosphere of rubber plants in West Kalimantan. Serial soil samples were diluted and cultured on ATCC no.14 medium to select potential bacteria to produce exopolysaccharides. Forty-five isolates of exopolysaccharide-producing bacteria isolated from the rhizosphere of rubber plants was inoculated on ATCC no.14 medium. Based on the observations of morphological colony of these isolates, most of them had similarities in colour and shape so that only ten different isolates were obtained based on the morphological colony. Ten isolates were re-grown on MacConcey medium. Three isolates formed thick or slimy mucus when cultured on MacConcey medium. Three isolates grown on the medium of ATCC 14 resulted in dry weight of exopolysaccharide (mg/mL) varying from 0.28 to 7.59 mg/mL with sucrose and glucose as carbon sources. The results of the molecular identification of the three isolates of Klebsiella sp. LW-13, Klebsiella pneumoniae strain DSM 30104 and Burkholderia anthina strain MYSP113 showed that Klebsiella sp. LW-13 and Burkholderia anthina strain MYSP113 with 2% organic matter increased soil aggregate stability from highly unstable (30.67%) to unstable (45.01-48.20%). This aligned with the results by scanning electron microscopy (SEM) on treated soil and without bacteria treatments. |
| first_indexed | 2024-12-16T08:29:35Z |
| format | Article |
| id | doaj.art-d849da7955674e8fbaeb3c165838afe9 |
| institution | Directory Open Access Journal |
| issn | 2339-076X 2502-2458 |
| language | English |
| last_indexed | 2024-12-16T08:29:35Z |
| publishDate | 2018-04-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Degraded and Mining Lands Management |
| spelling | doaj.art-d849da7955674e8fbaeb3c165838afe92022-12-21T22:37:54ZengUniversity of BrawijayaJournal of Degraded and Mining Lands Management2339-076X2502-24582018-04-01531275128110.15243/jdmlm.2018.053.1275182The potential of exopolysaccharide-producing bacteria from rhizosphere of rubber plants for improving soil aggregateNasrul Harahap0Dwi Andreas Santosa1Nuni Gofar2graduate student Bogor Agricultural UniverstyDepartment of Soil Science and Land Resources, Faculty of Agriculture, Bogor Agricultural University, BogorDepartment of Soil Science, Faculty of Agriculture, Sriwijaya University, PalembangThis study aimed to examine the effect of bacteria found in the rhizosphere of rubber plants in producing exopolysaccharides to improve aggregate stability of sandy soils. Samples of soil have been taken in rhizosphere of rubber plants in West Kalimantan. Serial soil samples were diluted and cultured on ATCC no.14 medium to select potential bacteria to produce exopolysaccharides. Forty-five isolates of exopolysaccharide-producing bacteria isolated from the rhizosphere of rubber plants was inoculated on ATCC no.14 medium. Based on the observations of morphological colony of these isolates, most of them had similarities in colour and shape so that only ten different isolates were obtained based on the morphological colony. Ten isolates were re-grown on MacConcey medium. Three isolates formed thick or slimy mucus when cultured on MacConcey medium. Three isolates grown on the medium of ATCC 14 resulted in dry weight of exopolysaccharide (mg/mL) varying from 0.28 to 7.59 mg/mL with sucrose and glucose as carbon sources. The results of the molecular identification of the three isolates of Klebsiella sp. LW-13, Klebsiella pneumoniae strain DSM 30104 and Burkholderia anthina strain MYSP113 showed that Klebsiella sp. LW-13 and Burkholderia anthina strain MYSP113 with 2% organic matter increased soil aggregate stability from highly unstable (30.67%) to unstable (45.01-48.20%). This aligned with the results by scanning electron microscopy (SEM) on treated soil and without bacteria treatments.http://jdmlm.ub.ac.id/index.php/jdmlm/article/view/427exopolysaccharides,Sandy soil, rubber, bacteria,soil aggregate |
| spellingShingle | Nasrul Harahap Dwi Andreas Santosa Nuni Gofar The potential of exopolysaccharide-producing bacteria from rhizosphere of rubber plants for improving soil aggregate Journal of Degraded and Mining Lands Management exopolysaccharides,Sandy soil, rubber, bacteria,soil aggregate |
| title | The potential of exopolysaccharide-producing bacteria from rhizosphere of rubber plants for improving soil aggregate |
| title_full | The potential of exopolysaccharide-producing bacteria from rhizosphere of rubber plants for improving soil aggregate |
| title_fullStr | The potential of exopolysaccharide-producing bacteria from rhizosphere of rubber plants for improving soil aggregate |
| title_full_unstemmed | The potential of exopolysaccharide-producing bacteria from rhizosphere of rubber plants for improving soil aggregate |
| title_short | The potential of exopolysaccharide-producing bacteria from rhizosphere of rubber plants for improving soil aggregate |
| title_sort | potential of exopolysaccharide producing bacteria from rhizosphere of rubber plants for improving soil aggregate |
| topic | exopolysaccharides,Sandy soil, rubber, bacteria,soil aggregate |
| url | http://jdmlm.ub.ac.id/index.php/jdmlm/article/view/427 |
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