ROS-Eliminating Carboxymethyl Chitosan Hydrogel to Enhance Burn Wound-Healing Efficacy
Overexpression of reactive oxygen species (ROS) can lead to chronic inflammation, which limits skin wound healing. Therefore, it is of great significance to develop materials that can locally control the adverse reactions caused by excessive ROS. In this research, an ROS-sensitive hydrogel with stro...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.679580/full |
| _version_ | 1819103661168525312 |
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| author | Cheng Yang Yuhui Chen Hai Huang Shicai Fan Chengliang Yang Liping Wang Wenqiang Li Wenxin Niu Jianwen Liao |
| author_facet | Cheng Yang Yuhui Chen Hai Huang Shicai Fan Chengliang Yang Liping Wang Wenqiang Li Wenxin Niu Jianwen Liao |
| author_sort | Cheng Yang |
| collection | DOAJ |
| description | Overexpression of reactive oxygen species (ROS) can lead to chronic inflammation, which limits skin wound healing. Therefore, it is of great significance to develop materials that can locally control the adverse reactions caused by excessive ROS. In this research, an ROS-sensitive hydrogel with strong free radical scavenging ability was prepared by introducing the thione (Tk) group into carboxymethyl chitosan (CMCTS) hydrogel. CMCTS hydrogel was cross-linked by NH2-Tk-NH2 agent and loaded curcumin (Cur), which possessed favorable nontoxicity, water absorption, mechanical property, biodegradability, drug release behavior, the M2 phenotype, and inflammatory factor regulating the capacity of macrophages. It is worth noting that Cur@CMCTS-Tk hydrogel can significantly inhibit oxidative damage of human fibroblasts in the H2O2-induced microenvironment and protect their viability by reducing the production of intracellular ROS. In vivo, ROS-removing hydrogel effectively accelerated the process of wound healing and possessed good regenerative properties, including hair follicle formation, promotion of new blood vessel formation, and highly orderly arrangement of collagen fibers in the full-thickness skin burn defect rat model. Hence, we expect that the Cur@CMCTS-Tk hydrogel could be used for wound treatment and tissue regeneration due to the ability to scavenge excess ROS. |
| first_indexed | 2024-12-22T01:54:00Z |
| format | Article |
| id | doaj.art-d84a3c97eebd4c51a3e57ee68eb80240 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-22T01:54:00Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-d84a3c97eebd4c51a3e57ee68eb802402022-12-21T18:42:50ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-06-011210.3389/fphar.2021.679580679580ROS-Eliminating Carboxymethyl Chitosan Hydrogel to Enhance Burn Wound-Healing EfficacyCheng Yang0Yuhui Chen1Hai Huang2Shicai Fan3Chengliang Yang4Liping Wang5Wenqiang Li6Wenxin Niu7Jianwen Liao8Center for Orthopaedic Surgery, Department of Orthopaedic Trauma, The Third Affiliated Hospital of Southern Medical University, Guangzhou, ChinaCenter for Orthopaedic Surgery, Department of Orthopaedic Trauma, The Third Affiliated Hospital of Southern Medical University, Guangzhou, ChinaCenter for Orthopaedic Surgery, Department of Orthopaedic Trauma, The Third Affiliated Hospital of Southern Medical University, Guangzhou, ChinaCenter for Orthopaedic Surgery, Department of Orthopaedic Trauma, The Third Affiliated Hospital of Southern Medical University, Guangzhou, ChinaDepartment of Orthopedics, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, ChinaUniSA Clinical & Health Science, UniSA Cancer Research Institute, University of South Australia, Adelaide, SA, AustraliaGungdong Provincial Engineering Technology Research Center for Sports Assistive Devices, Guangzhou Sport University, Guangzhou, ChinaYangzhi Rehabilitation Hospital, Tongji University School of Medicine, Shanghai, ChinaCenter for Orthopaedic Surgery, Department of Orthopaedic Trauma, The Third Affiliated Hospital of Southern Medical University, Guangzhou, ChinaOverexpression of reactive oxygen species (ROS) can lead to chronic inflammation, which limits skin wound healing. Therefore, it is of great significance to develop materials that can locally control the adverse reactions caused by excessive ROS. In this research, an ROS-sensitive hydrogel with strong free radical scavenging ability was prepared by introducing the thione (Tk) group into carboxymethyl chitosan (CMCTS) hydrogel. CMCTS hydrogel was cross-linked by NH2-Tk-NH2 agent and loaded curcumin (Cur), which possessed favorable nontoxicity, water absorption, mechanical property, biodegradability, drug release behavior, the M2 phenotype, and inflammatory factor regulating the capacity of macrophages. It is worth noting that Cur@CMCTS-Tk hydrogel can significantly inhibit oxidative damage of human fibroblasts in the H2O2-induced microenvironment and protect their viability by reducing the production of intracellular ROS. In vivo, ROS-removing hydrogel effectively accelerated the process of wound healing and possessed good regenerative properties, including hair follicle formation, promotion of new blood vessel formation, and highly orderly arrangement of collagen fibers in the full-thickness skin burn defect rat model. Hence, we expect that the Cur@CMCTS-Tk hydrogel could be used for wound treatment and tissue regeneration due to the ability to scavenge excess ROS.https://www.frontiersin.org/articles/10.3389/fphar.2021.679580/fullROS-sensitivecarboxymethyl chitosanhydrogelmacrophageswound healing |
| spellingShingle | Cheng Yang Yuhui Chen Hai Huang Shicai Fan Chengliang Yang Liping Wang Wenqiang Li Wenxin Niu Jianwen Liao ROS-Eliminating Carboxymethyl Chitosan Hydrogel to Enhance Burn Wound-Healing Efficacy Frontiers in Pharmacology ROS-sensitive carboxymethyl chitosan hydrogel macrophages wound healing |
| title | ROS-Eliminating Carboxymethyl Chitosan Hydrogel to Enhance Burn Wound-Healing Efficacy |
| title_full | ROS-Eliminating Carboxymethyl Chitosan Hydrogel to Enhance Burn Wound-Healing Efficacy |
| title_fullStr | ROS-Eliminating Carboxymethyl Chitosan Hydrogel to Enhance Burn Wound-Healing Efficacy |
| title_full_unstemmed | ROS-Eliminating Carboxymethyl Chitosan Hydrogel to Enhance Burn Wound-Healing Efficacy |
| title_short | ROS-Eliminating Carboxymethyl Chitosan Hydrogel to Enhance Burn Wound-Healing Efficacy |
| title_sort | ros eliminating carboxymethyl chitosan hydrogel to enhance burn wound healing efficacy |
| topic | ROS-sensitive carboxymethyl chitosan hydrogel macrophages wound healing |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2021.679580/full |
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