Sequencing and analysis of the complete mitochondrial genome of the masked shrew (Sorex caecutiens) from China

The complete mitogenome sequence of the masked shrew (Sorex caecutiens) was determined using long PCR. The genome was 17,096 bp in length and contained 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, one origin of L strand replication, and one control region. The overall bas...

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Main Authors: Zhi-Min Jin, Liu Zhu, Jian-Zhang Ma
Format: Article
Language:English
Published: Taylor & Francis Group 2017-12-01
Series:Mitochondrial DNA. Part B. Resources
Subjects:
Online Access:http://dx.doi.org/10.1080/23802359.2017.1361354
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author Zhi-Min Jin
Liu Zhu
Jian-Zhang Ma
author_facet Zhi-Min Jin
Liu Zhu
Jian-Zhang Ma
author_sort Zhi-Min Jin
collection DOAJ
description The complete mitogenome sequence of the masked shrew (Sorex caecutiens) was determined using long PCR. The genome was 17,096 bp in length and contained 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, one origin of L strand replication, and one control region. The overall base composition of the heavy strand is A (32.9%), C (24.5%), T (29.3%), and G (13.3%). The base compositions present clearly the A–T skew, which is most obviously in the control region and protein-coding genes. The extended termination-associated sequence domain, the central conserved domain and the conserved sequence block domain are defined in the mitochondrial genome control region of the masked shrew. Mitochondrial genome analyses based on MP, ML, NJ, and Bayesian analyses yielded identical phylogenetic trees. The five Sorex species formed a monophyletic group with the high bootstrap value (100%) in all examinations.
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spelling doaj.art-d84c2408cafe4ce4a569fca3bf2b6c962023-11-02T15:42:57ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592017-12-012248648810.1080/23802359.2017.13613541361354Sequencing and analysis of the complete mitochondrial genome of the masked shrew (Sorex caecutiens) from ChinaZhi-Min Jin0Liu Zhu1Jian-Zhang Ma2Northeast Forestry UniversityMudanjiang Normal UniversityNortheast Forestry UniversityThe complete mitogenome sequence of the masked shrew (Sorex caecutiens) was determined using long PCR. The genome was 17,096 bp in length and contained 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, one origin of L strand replication, and one control region. The overall base composition of the heavy strand is A (32.9%), C (24.5%), T (29.3%), and G (13.3%). The base compositions present clearly the A–T skew, which is most obviously in the control region and protein-coding genes. The extended termination-associated sequence domain, the central conserved domain and the conserved sequence block domain are defined in the mitochondrial genome control region of the masked shrew. Mitochondrial genome analyses based on MP, ML, NJ, and Bayesian analyses yielded identical phylogenetic trees. The five Sorex species formed a monophyletic group with the high bootstrap value (100%) in all examinations.http://dx.doi.org/10.1080/23802359.2017.1361354control regionmitogenomephylogenetic treesmasked shrew
spellingShingle Zhi-Min Jin
Liu Zhu
Jian-Zhang Ma
Sequencing and analysis of the complete mitochondrial genome of the masked shrew (Sorex caecutiens) from China
Mitochondrial DNA. Part B. Resources
control region
mitogenome
phylogenetic trees
masked shrew
title Sequencing and analysis of the complete mitochondrial genome of the masked shrew (Sorex caecutiens) from China
title_full Sequencing and analysis of the complete mitochondrial genome of the masked shrew (Sorex caecutiens) from China
title_fullStr Sequencing and analysis of the complete mitochondrial genome of the masked shrew (Sorex caecutiens) from China
title_full_unstemmed Sequencing and analysis of the complete mitochondrial genome of the masked shrew (Sorex caecutiens) from China
title_short Sequencing and analysis of the complete mitochondrial genome of the masked shrew (Sorex caecutiens) from China
title_sort sequencing and analysis of the complete mitochondrial genome of the masked shrew sorex caecutiens from china
topic control region
mitogenome
phylogenetic trees
masked shrew
url http://dx.doi.org/10.1080/23802359.2017.1361354
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