Chromatin structure in situ: the contribution of DNA ultrastructural cytochemistry
Ultrastructural studies conducted in situ using conventional transmission electron microscopy have had relatively little impact on defining the structural organization of chromatin. This is due to the fact that in routine transmission electron microscopy, together with the deoxyribonucleoprotein, ma...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2014-01-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://www.ejh.it/index.php/ejh/article/view/2307 |
| _version_ | 1819036831143952384 |
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| author | M. Derenzini A. L. Olins D. E. Olins |
| author_facet | M. Derenzini A. L. Olins D. E. Olins |
| author_sort | M. Derenzini |
| collection | DOAJ |
| description | Ultrastructural studies conducted in situ using conventional transmission electron microscopy have had relatively little impact on defining the structural organization of chromatin. This is due to the fact that in routine transmission electron microscopy, together with the deoxyribonucleoprotein, many different intermingled substances are contrasted, masking the ultrastructure of chromatin. By selective staining of DNA in thin sections, using the Feulgen-like osmium-ammine reaction, these drawbacks have been overcome and worthwhile data have been obtained both on the gross morphology and the ultrastructural-functional organization of chromatin in situ. In the present study these results are reviewed and discussed in light of recent achievements in both interphase nuclear chromatin compartmentalization in interphase nuclei and in the structural organization of chromatin fibers in transcriptionally active and inactive chromatin. |
| first_indexed | 2024-12-21T08:11:46Z |
| format | Article |
| id | doaj.art-d84dec357be84e6d882ff2cd3c785327 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-21T08:11:46Z |
| publishDate | 2014-01-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-d84dec357be84e6d882ff2cd3c7853272022-12-21T19:10:38ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062014-01-0158110.4081/ejh.2014.23071381Chromatin structure in situ: the contribution of DNA ultrastructural cytochemistryM. Derenzini0A. L. Olins1D. E. Olins2University of BolognaUniversity of New EnglandUniversity of New EnglandUltrastructural studies conducted in situ using conventional transmission electron microscopy have had relatively little impact on defining the structural organization of chromatin. This is due to the fact that in routine transmission electron microscopy, together with the deoxyribonucleoprotein, many different intermingled substances are contrasted, masking the ultrastructure of chromatin. By selective staining of DNA in thin sections, using the Feulgen-like osmium-ammine reaction, these drawbacks have been overcome and worthwhile data have been obtained both on the gross morphology and the ultrastructural-functional organization of chromatin in situ. In the present study these results are reviewed and discussed in light of recent achievements in both interphase nuclear chromatin compartmentalization in interphase nuclei and in the structural organization of chromatin fibers in transcriptionally active and inactive chromatin.http://www.ejh.it/index.php/ejh/article/view/2307chromatin structure in situ, DNA cytochemistry, transmission electron microscopy, chromatin fibers, nucleosomes, osmium-ammine |
| spellingShingle | M. Derenzini A. L. Olins D. E. Olins Chromatin structure in situ: the contribution of DNA ultrastructural cytochemistry European Journal of Histochemistry chromatin structure in situ, DNA cytochemistry, transmission electron microscopy, chromatin fibers, nucleosomes, osmium-ammine |
| title | Chromatin structure in situ: the contribution of DNA ultrastructural cytochemistry |
| title_full | Chromatin structure in situ: the contribution of DNA ultrastructural cytochemistry |
| title_fullStr | Chromatin structure in situ: the contribution of DNA ultrastructural cytochemistry |
| title_full_unstemmed | Chromatin structure in situ: the contribution of DNA ultrastructural cytochemistry |
| title_short | Chromatin structure in situ: the contribution of DNA ultrastructural cytochemistry |
| title_sort | chromatin structure in situ the contribution of dna ultrastructural cytochemistry |
| topic | chromatin structure in situ, DNA cytochemistry, transmission electron microscopy, chromatin fibers, nucleosomes, osmium-ammine |
| url | http://www.ejh.it/index.php/ejh/article/view/2307 |
| work_keys_str_mv | AT mderenzini chromatinstructureinsituthecontributionofdnaultrastructuralcytochemistry AT alolins chromatinstructureinsituthecontributionofdnaultrastructuralcytochemistry AT deolins chromatinstructureinsituthecontributionofdnaultrastructuralcytochemistry |