A model symbiosis reveals a role for sheathed-flagellum rotation in the release of immunogenic lipopolysaccharide
Bacterial flagella mediate host–microbe interactions through tissue tropism during colonization, as well as by activating immune responses. The flagellar shaft of some bacteria, including several human pathogens, is encased in a membranous sheath of unknown function. While it has been hypothesized t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2014-03-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/01579 |
| _version_ | 1811200131224043520 |
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| author | Caitlin A Brennan Jason R Hunt Natacha Kremer Benjamin C Krasity Michael A Apicella Margaret J McFall-Ngai Edward G Ruby |
| author_facet | Caitlin A Brennan Jason R Hunt Natacha Kremer Benjamin C Krasity Michael A Apicella Margaret J McFall-Ngai Edward G Ruby |
| author_sort | Caitlin A Brennan |
| collection | DOAJ |
| description | Bacterial flagella mediate host–microbe interactions through tissue tropism during colonization, as well as by activating immune responses. The flagellar shaft of some bacteria, including several human pathogens, is encased in a membranous sheath of unknown function. While it has been hypothesized that the sheath may allow these bacteria to evade host responses to the immunogenic flagellin subunit, this unusual structural feature has remained an enigma. Here we demonstrate that the rotation of the sheathed flagellum in both the mutualist Vibrio fischeri and the pathogen Vibrio cholerae promotes release of a potent bacteria-derived immunogen, lipopolysaccharide, found in the flagellar sheath. We further present a new role for the flagellar sheath in triggering, rather than circumventing, host immune responses in the model squid-vibrio symbiosis. Such an observation not only has implications for the study of bacterial pathogens with sheathed flagella, but also raises important biophysical questions of sheathed-flagellum function. |
| first_indexed | 2024-04-12T01:59:41Z |
| format | Article |
| id | doaj.art-d84ed2372c1f42f096c3ae95631b9cb1 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:59:41Z |
| publishDate | 2014-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-d84ed2372c1f42f096c3ae95631b9cb12022-12-22T03:52:43ZengeLife Sciences Publications LtdeLife2050-084X2014-03-01310.7554/eLife.01579A model symbiosis reveals a role for sheathed-flagellum rotation in the release of immunogenic lipopolysaccharideCaitlin A Brennan0Jason R Hunt1Natacha Kremer2Benjamin C Krasity3Michael A Apicella4Margaret J McFall-Ngai5Edward G Ruby6Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, United StatesDepartment of Microbiology, The Carver College of Medicine, University of Iowa, Iowa City, United States; Department of Internal Medicine, The Carver College of Medicine, University of Iowa, Iowa City, United StatesDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, United StatesDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, United StatesDepartment of Microbiology, The Carver College of Medicine, University of Iowa, Iowa City, United States; Department of Internal Medicine, The Carver College of Medicine, University of Iowa, Iowa City, United StatesDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, United StatesDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, United StatesBacterial flagella mediate host–microbe interactions through tissue tropism during colonization, as well as by activating immune responses. The flagellar shaft of some bacteria, including several human pathogens, is encased in a membranous sheath of unknown function. While it has been hypothesized that the sheath may allow these bacteria to evade host responses to the immunogenic flagellin subunit, this unusual structural feature has remained an enigma. Here we demonstrate that the rotation of the sheathed flagellum in both the mutualist Vibrio fischeri and the pathogen Vibrio cholerae promotes release of a potent bacteria-derived immunogen, lipopolysaccharide, found in the flagellar sheath. We further present a new role for the flagellar sheath in triggering, rather than circumventing, host immune responses in the model squid-vibrio symbiosis. Such an observation not only has implications for the study of bacterial pathogens with sheathed flagella, but also raises important biophysical questions of sheathed-flagellum function.https://elifesciences.org/articles/01579Vibrio fischericholeraeflagellaLPS |
| spellingShingle | Caitlin A Brennan Jason R Hunt Natacha Kremer Benjamin C Krasity Michael A Apicella Margaret J McFall-Ngai Edward G Ruby A model symbiosis reveals a role for sheathed-flagellum rotation in the release of immunogenic lipopolysaccharide eLife Vibrio fischeri cholerae flagella LPS |
| title | A model symbiosis reveals a role for sheathed-flagellum rotation in the release of immunogenic lipopolysaccharide |
| title_full | A model symbiosis reveals a role for sheathed-flagellum rotation in the release of immunogenic lipopolysaccharide |
| title_fullStr | A model symbiosis reveals a role for sheathed-flagellum rotation in the release of immunogenic lipopolysaccharide |
| title_full_unstemmed | A model symbiosis reveals a role for sheathed-flagellum rotation in the release of immunogenic lipopolysaccharide |
| title_short | A model symbiosis reveals a role for sheathed-flagellum rotation in the release of immunogenic lipopolysaccharide |
| title_sort | model symbiosis reveals a role for sheathed flagellum rotation in the release of immunogenic lipopolysaccharide |
| topic | Vibrio fischeri cholerae flagella LPS |
| url | https://elifesciences.org/articles/01579 |
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