Antiallergic Effects of <i>Callerya atropurpurea</i> Extract In Vitro and in an In Vivo Atopic Dermatitis Model
(1) Background: <i>Callerya atropurpurea</i> is found in Laos, Thailand, and Vietnam. Although the anti-inflammatory action of <i>C. atropurpurea</i> has been investigated, the functions of this plant in allergic responses are not understood. Here, we explored the antiallergi...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/4/860 |
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| author | Wooram Choi Hwa Pyoung Lee Philaxay Manilack Veosavanh Saysavanh Byoung-Hee Lee Sarah Lee Eunji Kim Jae Youl Cho |
| author_facet | Wooram Choi Hwa Pyoung Lee Philaxay Manilack Veosavanh Saysavanh Byoung-Hee Lee Sarah Lee Eunji Kim Jae Youl Cho |
| author_sort | Wooram Choi |
| collection | DOAJ |
| description | (1) Background: <i>Callerya atropurpurea</i> is found in Laos, Thailand, and Vietnam. Although the anti-inflammatory action of <i>C. atropurpurea</i> has been investigated, the functions of this plant in allergic responses are not understood. Here, we explored the antiallergic mechanism of <i>C. atropurpurea</i> ethanol extract (Ca-EE) using in vitro assays and an in vivo atopic model. (2) Methods: The constituents of Ca-EE were analyzed using GC/MS. Inhibition of lipoxygenase and β-hexosaminidase activity was examined, and the expression of inflammatory genes was measured by quantitative real-time PCR. The regulatory roles of Ca-EE in IgE/FcεRI signaling were examined by Western blotting. The DNCB-induced atopic dermatitis mouse model was performed with histological analysis. (3) Results: Ca-EE comprised cis-raphasatin, lupeol, some sugars, and fatty acids. In RBL-2H3 cells, treatment with Ca-EE significantly reduced the activities of lipoxygenase and β-hexosaminidase, as well as cytokine gene expression. IgE-mediated signaling was downregulated by blocking Lyn kinases. Moreover, Ca-EE effectively inhibited allergic symptoms in the DNCB-induced atopic dermatitis model without toxicity. (4) Conclusions: Ca-EE displayed antiallergic activities through regulating IgE/Lyn signaling in RBL-2H3 cells and a contact dermatitis model. These results indicate that Ca-EE could be effective for allergic disease treatment. |
| first_indexed | 2024-03-11T08:15:15Z |
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| language | English |
| last_indexed | 2024-03-11T08:15:15Z |
| publishDate | 2023-02-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-d850e1326d38455392b5c6d6019a23962023-11-16T22:48:27ZengMDPI AGPlants2223-77472023-02-0112486010.3390/plants12040860Antiallergic Effects of <i>Callerya atropurpurea</i> Extract In Vitro and in an In Vivo Atopic Dermatitis ModelWooram Choi0Hwa Pyoung Lee1Philaxay Manilack2Veosavanh Saysavanh3Byoung-Hee Lee4Sarah Lee5Eunji Kim6Jae Youl Cho7Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Republic of KoreaDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Republic of KoreaDepartment of Forestry, Ministry of Agriculture and Forestry, Vientiane P.O. Box 811, LaosDepartment of Forestry, Ministry of Agriculture and Forestry, Vientiane P.O. Box 811, LaosNational Institute of Biological Resources, Environmental Research Complex, Incheon 22689, Republic of KoreaNational Institute of Biological Resources, Environmental Research Complex, Incheon 22689, Republic of KoreaDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Republic of KoreaDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Republic of Korea(1) Background: <i>Callerya atropurpurea</i> is found in Laos, Thailand, and Vietnam. Although the anti-inflammatory action of <i>C. atropurpurea</i> has been investigated, the functions of this plant in allergic responses are not understood. Here, we explored the antiallergic mechanism of <i>C. atropurpurea</i> ethanol extract (Ca-EE) using in vitro assays and an in vivo atopic model. (2) Methods: The constituents of Ca-EE were analyzed using GC/MS. Inhibition of lipoxygenase and β-hexosaminidase activity was examined, and the expression of inflammatory genes was measured by quantitative real-time PCR. The regulatory roles of Ca-EE in IgE/FcεRI signaling were examined by Western blotting. The DNCB-induced atopic dermatitis mouse model was performed with histological analysis. (3) Results: Ca-EE comprised cis-raphasatin, lupeol, some sugars, and fatty acids. In RBL-2H3 cells, treatment with Ca-EE significantly reduced the activities of lipoxygenase and β-hexosaminidase, as well as cytokine gene expression. IgE-mediated signaling was downregulated by blocking Lyn kinases. Moreover, Ca-EE effectively inhibited allergic symptoms in the DNCB-induced atopic dermatitis model without toxicity. (4) Conclusions: Ca-EE displayed antiallergic activities through regulating IgE/Lyn signaling in RBL-2H3 cells and a contact dermatitis model. These results indicate that Ca-EE could be effective for allergic disease treatment.https://www.mdpi.com/2223-7747/12/4/860<i>Callerya atropurpurea</i>allergyatopic dermatitisLyn kinase |
| spellingShingle | Wooram Choi Hwa Pyoung Lee Philaxay Manilack Veosavanh Saysavanh Byoung-Hee Lee Sarah Lee Eunji Kim Jae Youl Cho Antiallergic Effects of <i>Callerya atropurpurea</i> Extract In Vitro and in an In Vivo Atopic Dermatitis Model Plants <i>Callerya atropurpurea</i> allergy atopic dermatitis Lyn kinase |
| title | Antiallergic Effects of <i>Callerya atropurpurea</i> Extract In Vitro and in an In Vivo Atopic Dermatitis Model |
| title_full | Antiallergic Effects of <i>Callerya atropurpurea</i> Extract In Vitro and in an In Vivo Atopic Dermatitis Model |
| title_fullStr | Antiallergic Effects of <i>Callerya atropurpurea</i> Extract In Vitro and in an In Vivo Atopic Dermatitis Model |
| title_full_unstemmed | Antiallergic Effects of <i>Callerya atropurpurea</i> Extract In Vitro and in an In Vivo Atopic Dermatitis Model |
| title_short | Antiallergic Effects of <i>Callerya atropurpurea</i> Extract In Vitro and in an In Vivo Atopic Dermatitis Model |
| title_sort | antiallergic effects of i callerya atropurpurea i extract in vitro and in an in vivo atopic dermatitis model |
| topic | <i>Callerya atropurpurea</i> allergy atopic dermatitis Lyn kinase |
| url | https://www.mdpi.com/2223-7747/12/4/860 |
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