Genomic and Phenotypic Insights Into the Potential of Rock Phosphate Solubilizing Bacteria to Promote Millet Growth in vivo
Rock phosphate (RP) is a natural source of phosphorus for agriculture, with the advantage of lower cost and less impact on the environment when compared to synthetic fertilizers. However, the release of phosphorus (P) from RP occurs slowly, which may limit its short-term availability to crops. Hence...
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.574550/full |
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| author | Ubiana C. Silva Sara Cuadros-Orellana Daliane R. C. Silva Luiz F. Freitas-Júnior Ana C. Fernandes Laura R. Leite Christiane A. Oliveira Vera L. Dos Santos |
| author_facet | Ubiana C. Silva Sara Cuadros-Orellana Daliane R. C. Silva Luiz F. Freitas-Júnior Ana C. Fernandes Laura R. Leite Christiane A. Oliveira Vera L. Dos Santos |
| author_sort | Ubiana C. Silva |
| collection | DOAJ |
| description | Rock phosphate (RP) is a natural source of phosphorus for agriculture, with the advantage of lower cost and less impact on the environment when compared to synthetic fertilizers. However, the release of phosphorus (P) from RP occurs slowly, which may limit its short-term availability to crops. Hence, the use of P-solubilizing microorganisms to improve the availability of P from this P source is an interesting approach, as microorganisms often perform other functions that assist plant growth, besides solubilizing P. Here, we describe the characterization of 101 bacterial isolates obtained from the rhizosphere and endosphere of maize plants for their P solubilizing activity in vitro, their growth-promoting activity on millet plants cultivated in soil amended with RP, and their gene content especially associated with phosphate solubilization. For the in vitro solubilization assays, two mineral P sources were used: rock phosphate from Araxá (Brazil) mine (AP) and iron phosphate (Fe-P). The amounts of P released from Fe–P in the solubilization assays were lower than those released from AP, and the endophytic bacteria outperformed the rhizospheric ones in the solubilization of both P sources. Six selected strains were evaluated for their ability to promote the growth of millet in soil fertilized with a commercial rock phosphate (cRP). Two of them, namely Bacillus megaterium UFMG50 and Ochrobactrum pseudogrignonense CNPMS2088, performed better than the others in the cRP assays, improving at least six physiological traits of millet or P content in the soil. Genomic analysis of these bacteria revealed the presence of genes related to P uptake and metabolism, and to organic acid synthesis. Using this approach, we identified six potential candidates as bioinoculants, which are promising for use under field conditions, as they have both the genetic potential and the experimentally demonstrated in vivo ability to improve rock phosphate solubilization and promote plant growth. |
| first_indexed | 2024-12-14T14:55:32Z |
| format | Article |
| id | doaj.art-d85553684feb498a8b0c7027807b1daf |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T14:55:32Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-d85553684feb498a8b0c7027807b1daf2022-12-21T22:57:00ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-01-011110.3389/fmicb.2020.574550574550Genomic and Phenotypic Insights Into the Potential of Rock Phosphate Solubilizing Bacteria to Promote Millet Growth in vivoUbiana C. Silva0Sara Cuadros-Orellana1Daliane R. C. Silva2Luiz F. Freitas-Júnior3Ana C. Fernandes4Laura R. Leite5Christiane A. Oliveira6Vera L. Dos Santos7Department of Microbiology, Universidade Federal de Minas Gerais, Belo Horizonte, BrazilCentro de Biotecnología de los Recursos Naturales, Universidad Católica del Maule, Talca, ChileDepartment of Microbiology, Universidade Federal de Minas Gerais, Belo Horizonte, BrazilDepartment of Microbiology, Universidade Federal de Minas Gerais, Belo Horizonte, BrazilDepartment of Microbiology, Universidade Federal de Minas Gerais, Belo Horizonte, BrazilGrupo de Genômica e Informática de Biossistemas, Centro de Pesquisas René Rachou, Fiocruz, Belo Horizonte, BrazilEmbrapa Milho e Sorgo, Sete Lagoas, BrazilDepartment of Microbiology, Universidade Federal de Minas Gerais, Belo Horizonte, BrazilRock phosphate (RP) is a natural source of phosphorus for agriculture, with the advantage of lower cost and less impact on the environment when compared to synthetic fertilizers. However, the release of phosphorus (P) from RP occurs slowly, which may limit its short-term availability to crops. Hence, the use of P-solubilizing microorganisms to improve the availability of P from this P source is an interesting approach, as microorganisms often perform other functions that assist plant growth, besides solubilizing P. Here, we describe the characterization of 101 bacterial isolates obtained from the rhizosphere and endosphere of maize plants for their P solubilizing activity in vitro, their growth-promoting activity on millet plants cultivated in soil amended with RP, and their gene content especially associated with phosphate solubilization. For the in vitro solubilization assays, two mineral P sources were used: rock phosphate from Araxá (Brazil) mine (AP) and iron phosphate (Fe-P). The amounts of P released from Fe–P in the solubilization assays were lower than those released from AP, and the endophytic bacteria outperformed the rhizospheric ones in the solubilization of both P sources. Six selected strains were evaluated for their ability to promote the growth of millet in soil fertilized with a commercial rock phosphate (cRP). Two of them, namely Bacillus megaterium UFMG50 and Ochrobactrum pseudogrignonense CNPMS2088, performed better than the others in the cRP assays, improving at least six physiological traits of millet or P content in the soil. Genomic analysis of these bacteria revealed the presence of genes related to P uptake and metabolism, and to organic acid synthesis. Using this approach, we identified six potential candidates as bioinoculants, which are promising for use under field conditions, as they have both the genetic potential and the experimentally demonstrated in vivo ability to improve rock phosphate solubilization and promote plant growth.https://www.frontiersin.org/articles/10.3389/fmicb.2020.574550/fullbacteriaphosphatesolubilizationmaizeplant growth promotion |
| spellingShingle | Ubiana C. Silva Sara Cuadros-Orellana Daliane R. C. Silva Luiz F. Freitas-Júnior Ana C. Fernandes Laura R. Leite Christiane A. Oliveira Vera L. Dos Santos Genomic and Phenotypic Insights Into the Potential of Rock Phosphate Solubilizing Bacteria to Promote Millet Growth in vivo Frontiers in Microbiology bacteria phosphate solubilization maize plant growth promotion |
| title | Genomic and Phenotypic Insights Into the Potential of Rock Phosphate Solubilizing Bacteria to Promote Millet Growth in vivo |
| title_full | Genomic and Phenotypic Insights Into the Potential of Rock Phosphate Solubilizing Bacteria to Promote Millet Growth in vivo |
| title_fullStr | Genomic and Phenotypic Insights Into the Potential of Rock Phosphate Solubilizing Bacteria to Promote Millet Growth in vivo |
| title_full_unstemmed | Genomic and Phenotypic Insights Into the Potential of Rock Phosphate Solubilizing Bacteria to Promote Millet Growth in vivo |
| title_short | Genomic and Phenotypic Insights Into the Potential of Rock Phosphate Solubilizing Bacteria to Promote Millet Growth in vivo |
| title_sort | genomic and phenotypic insights into the potential of rock phosphate solubilizing bacteria to promote millet growth in vivo |
| topic | bacteria phosphate solubilization maize plant growth promotion |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.574550/full |
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