Saussurea lappa plant rhizome extract-based zinc oxide nanoparticles: synthesis, characterization and its antibacterial, antifungal activities and cytotoxic studies against Chinese Hamster Ovary (CHO) cell lines
The plant extracts are known for their anti-inflammatory, antifungal, antiviral and antibacterial properties. The use of plant extracts in the preparation of bio-materials increases their biological application. In this concern, herein reporting an eco-friendly procedure which is also a simple and c...
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| Format: | Article |
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Elsevier
2021-06-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844021013682 |
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| author | Lalitha A. Kolahalam K.R.S. Prasad P. Murali Krishna N. Supraja |
| author_facet | Lalitha A. Kolahalam K.R.S. Prasad P. Murali Krishna N. Supraja |
| author_sort | Lalitha A. Kolahalam |
| collection | DOAJ |
| description | The plant extracts are known for their anti-inflammatory, antifungal, antiviral and antibacterial properties. The use of plant extracts in the preparation of bio-materials increases their biological application. In this concern, herein reporting an eco-friendly procedure which is also a simple and cost effective, for the synthesis of Zinc Oxide nanoparticles (ZnONPs) using Saussurea lappa plant root (rhizome) extract as a fuel. The prepared nanoparticles were confirmed using various characterization techniques. The Dynamic light scattering data showed 123.5 nm particle size with -99.9 mv zeta potential which indicates excellent stability of the particles. The peak at 541 cm−1 in the IR spectrum is assigned to the stretching frequency of the zinc-binding to oxygen. The X-ray diffraction peaks confirm the close association with JCPDS Data Card No: 36-1451. The FESEM data revealed a hexagonal wurtzite structure with a hexagonal shape of synthesized ZnO nanoparticles. The antibacterial studies indicate the gram-negative strains showed better inhibition activity than gram-positive strains. Among Fungal strains, Aspergillus niger and flavus, Fusarium oxysporum, and Rhizopus oryzae showed good inhibition activity at higher concentrations. The cytotoxic data indicates the 5 μg/mL of the ZnO particles showed cytotoxicity on the CHO cell line and with IC50 value 3.164 ± 0.8956 μg/mL. |
| first_indexed | 2024-12-17T03:24:24Z |
| format | Article |
| id | doaj.art-d855dfd2030b40d9a2dbc19be8377a52 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-17T03:24:24Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-d855dfd2030b40d9a2dbc19be8377a522022-12-21T22:05:26ZengElsevierHeliyon2405-84402021-06-0176e07265Saussurea lappa plant rhizome extract-based zinc oxide nanoparticles: synthesis, characterization and its antibacterial, antifungal activities and cytotoxic studies against Chinese Hamster Ovary (CHO) cell linesLalitha A. Kolahalam0K.R.S. Prasad1P. Murali Krishna2N. Supraja3Department of Chemistry, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, 522502, Andhra Pradesh, IndiaDepartment of Chemistry, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, 522502, Andhra Pradesh, India; Corresponding author.Department of Chemistry, Ramaiah Institute of Technology, Bangalore, 560054, Karnataka, India; Corresponding author.Nanotechnology Laboratory, Acharya N G Ranga Agricultural University, Tirupati, 517502, Andhra Pradesh, IndiaThe plant extracts are known for their anti-inflammatory, antifungal, antiviral and antibacterial properties. The use of plant extracts in the preparation of bio-materials increases their biological application. In this concern, herein reporting an eco-friendly procedure which is also a simple and cost effective, for the synthesis of Zinc Oxide nanoparticles (ZnONPs) using Saussurea lappa plant root (rhizome) extract as a fuel. The prepared nanoparticles were confirmed using various characterization techniques. The Dynamic light scattering data showed 123.5 nm particle size with -99.9 mv zeta potential which indicates excellent stability of the particles. The peak at 541 cm−1 in the IR spectrum is assigned to the stretching frequency of the zinc-binding to oxygen. The X-ray diffraction peaks confirm the close association with JCPDS Data Card No: 36-1451. The FESEM data revealed a hexagonal wurtzite structure with a hexagonal shape of synthesized ZnO nanoparticles. The antibacterial studies indicate the gram-negative strains showed better inhibition activity than gram-positive strains. Among Fungal strains, Aspergillus niger and flavus, Fusarium oxysporum, and Rhizopus oryzae showed good inhibition activity at higher concentrations. The cytotoxic data indicates the 5 μg/mL of the ZnO particles showed cytotoxicity on the CHO cell line and with IC50 value 3.164 ± 0.8956 μg/mL.http://www.sciencedirect.com/science/article/pii/S2405844021013682ZnO nanoparticlesSaussurea lappaAntimicrobial activityCytotoxic activity |
| spellingShingle | Lalitha A. Kolahalam K.R.S. Prasad P. Murali Krishna N. Supraja Saussurea lappa plant rhizome extract-based zinc oxide nanoparticles: synthesis, characterization and its antibacterial, antifungal activities and cytotoxic studies against Chinese Hamster Ovary (CHO) cell lines Heliyon ZnO nanoparticles Saussurea lappa Antimicrobial activity Cytotoxic activity |
| title | Saussurea lappa plant rhizome extract-based zinc oxide nanoparticles: synthesis, characterization and its antibacterial, antifungal activities and cytotoxic studies against Chinese Hamster Ovary (CHO) cell lines |
| title_full | Saussurea lappa plant rhizome extract-based zinc oxide nanoparticles: synthesis, characterization and its antibacterial, antifungal activities and cytotoxic studies against Chinese Hamster Ovary (CHO) cell lines |
| title_fullStr | Saussurea lappa plant rhizome extract-based zinc oxide nanoparticles: synthesis, characterization and its antibacterial, antifungal activities and cytotoxic studies against Chinese Hamster Ovary (CHO) cell lines |
| title_full_unstemmed | Saussurea lappa plant rhizome extract-based zinc oxide nanoparticles: synthesis, characterization and its antibacterial, antifungal activities and cytotoxic studies against Chinese Hamster Ovary (CHO) cell lines |
| title_short | Saussurea lappa plant rhizome extract-based zinc oxide nanoparticles: synthesis, characterization and its antibacterial, antifungal activities and cytotoxic studies against Chinese Hamster Ovary (CHO) cell lines |
| title_sort | saussurea lappa plant rhizome extract based zinc oxide nanoparticles synthesis characterization and its antibacterial antifungal activities and cytotoxic studies against chinese hamster ovary cho cell lines |
| topic | ZnO nanoparticles Saussurea lappa Antimicrobial activity Cytotoxic activity |
| url | http://www.sciencedirect.com/science/article/pii/S2405844021013682 |
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