Antioxidant activity relationship of phenolic compounds in <it>Hypericum perforatum </it>L.
<p>Abstract</p> <p>Background</p> <p>The St John's Wort (<it>Hypericum perforatum</it>; Clusiaceae) has been used in traditional and modern medicine for a long time due to its high content of biologically active phenolics. The purpose of this work was t...
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| Format: | Article |
| Language: | English |
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BMC
2011-06-01
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| Series: | Chemistry Central Journal |
| Online Access: | http://journal.chemistrycentral.com/content/5/1/34 |
| _version_ | 1818790748457271296 |
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| author | Petrović Slobodan S Francišković Marina M Mimica-Dukić Neda M Orčić Dejan Z Jovin Emilija Đ |
| author_facet | Petrović Slobodan S Francišković Marina M Mimica-Dukić Neda M Orčić Dejan Z Jovin Emilija Đ |
| author_sort | Petrović Slobodan S |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The St John's Wort (<it>Hypericum perforatum</it>; Clusiaceae) has been used in traditional and modern medicine for a long time due to its high content of biologically active phenolics. The purpose of this work was to develop a method for their fractionation and identification, and to determine the most active antioxidant compounds in plant extract.</p> <p>Results</p> <p>An LC-MS method which enables fast qualitative and semiquantitative analysis was developed. The composition determined is in agreement with the previous results, where 6 flavonoids, 4 naphthodianthrones and 4 phloroglucinols have been identified. Significant antioxidant activity was determined for most of the fractions by DPPH assay (the lowest IC<sub>50 </sub>of 0.52 μg/ml), NO scavenging (6.11 μg/ml), superoxide scavenging (1.86 μg/ml), lipid peroxidation (0.0079 μg/ml) and FRAP (the highest reduction capacity of 104 mg Fe equivalents/g) assays.</p> <p>Conclusion</p> <p>LC-MS technique has been successfully applied for a quick separation and identification of the major components of <it>H. perforatum </it>fractions. Majority of the fractions analyzed have expressed a very high antioxidative activity when compared to synthetic antioxidants. The antioxidant activity could be attributed to flavonoids and phenolic acids, while phloroglucinols and naphthodianthrones showed no significant activity. It is demonstrated that it is possible to obtain, by fractionation, <it>H. perforatum </it>preparations with significantly increased phloroglucinols-to-naphthodianthrones ratio (up to 95:5).</p> |
| first_indexed | 2024-12-18T15:00:23Z |
| format | Article |
| id | doaj.art-d85840d44586489f87e758742ddebaec |
| institution | Directory Open Access Journal |
| issn | 1752-153X |
| language | English |
| last_indexed | 2024-12-18T15:00:23Z |
| publishDate | 2011-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Chemistry Central Journal |
| spelling | doaj.art-d85840d44586489f87e758742ddebaec2022-12-21T21:03:55ZengBMCChemistry Central Journal1752-153X2011-06-01513410.1186/1752-153X-5-34Antioxidant activity relationship of phenolic compounds in <it>Hypericum perforatum </it>L.Petrović Slobodan SFrancišković Marina MMimica-Dukić Neda MOrčić Dejan ZJovin Emilija Đ<p>Abstract</p> <p>Background</p> <p>The St John's Wort (<it>Hypericum perforatum</it>; Clusiaceae) has been used in traditional and modern medicine for a long time due to its high content of biologically active phenolics. The purpose of this work was to develop a method for their fractionation and identification, and to determine the most active antioxidant compounds in plant extract.</p> <p>Results</p> <p>An LC-MS method which enables fast qualitative and semiquantitative analysis was developed. The composition determined is in agreement with the previous results, where 6 flavonoids, 4 naphthodianthrones and 4 phloroglucinols have been identified. Significant antioxidant activity was determined for most of the fractions by DPPH assay (the lowest IC<sub>50 </sub>of 0.52 μg/ml), NO scavenging (6.11 μg/ml), superoxide scavenging (1.86 μg/ml), lipid peroxidation (0.0079 μg/ml) and FRAP (the highest reduction capacity of 104 mg Fe equivalents/g) assays.</p> <p>Conclusion</p> <p>LC-MS technique has been successfully applied for a quick separation and identification of the major components of <it>H. perforatum </it>fractions. Majority of the fractions analyzed have expressed a very high antioxidative activity when compared to synthetic antioxidants. The antioxidant activity could be attributed to flavonoids and phenolic acids, while phloroglucinols and naphthodianthrones showed no significant activity. It is demonstrated that it is possible to obtain, by fractionation, <it>H. perforatum </it>preparations with significantly increased phloroglucinols-to-naphthodianthrones ratio (up to 95:5).</p>http://journal.chemistrycentral.com/content/5/1/34 |
| spellingShingle | Petrović Slobodan S Francišković Marina M Mimica-Dukić Neda M Orčić Dejan Z Jovin Emilija Đ Antioxidant activity relationship of phenolic compounds in <it>Hypericum perforatum </it>L. Chemistry Central Journal |
| title | Antioxidant activity relationship of phenolic compounds in <it>Hypericum perforatum </it>L. |
| title_full | Antioxidant activity relationship of phenolic compounds in <it>Hypericum perforatum </it>L. |
| title_fullStr | Antioxidant activity relationship of phenolic compounds in <it>Hypericum perforatum </it>L. |
| title_full_unstemmed | Antioxidant activity relationship of phenolic compounds in <it>Hypericum perforatum </it>L. |
| title_short | Antioxidant activity relationship of phenolic compounds in <it>Hypericum perforatum </it>L. |
| title_sort | antioxidant activity relationship of phenolic compounds in it hypericum perforatum it l |
| url | http://journal.chemistrycentral.com/content/5/1/34 |
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