Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize on Storage Pest <i>Paralipsa gularis</i> (Zeller)
<i>Paralipsa gularis</i> (Zeller) is a storage pest; however, in recent years it has evolved into a considerable maize pest during the late growth stage in the border region between China and other Southeast Asian countries. Bt transgenic insect-resistant maize is an effective measure in...
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MDPI AG
2024-02-01
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| Series: | Toxins |
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| author | Shuang Chen Wenhui Wang Guodong Kang Xianming Yang Kongming Wu |
| author_facet | Shuang Chen Wenhui Wang Guodong Kang Xianming Yang Kongming Wu |
| author_sort | Shuang Chen |
| collection | DOAJ |
| description | <i>Paralipsa gularis</i> (Zeller) is a storage pest; however, in recent years it has evolved into a considerable maize pest during the late growth stage in the border region between China and other Southeast Asian countries. Bt transgenic insect-resistant maize is an effective measure in controlling a wide range of lepidopteran pests, but there is a lack of research on the toxic effects of storage pests. We tested the toxicity of Bt-Cry1Ab, Vip3Aa, and their complex proteins against <i>P. gularis</i> via bioassay and investigated the efficiency of Bt-(Cry1Ab+Vip3Aa) maize in controlling <i>P. gularis</i> during the late growth stage of maize in the period 2022–2023. The bioassay results show that the susceptibilities of <i>P. gularis</i> to the two Bt proteins and their complex proteins were significantly different. The LC<sub>50</sub> values of DBNCry1Ab (“DBN9936” event), DBNVip3Aa (“DBN9501” event), DBN Cry1Ab+Vip3Aa (“DBN3601T” event), and Syngenta Cry1Ab+Vip3Aa (“Bt11” event × “MIR162” event) were 0.038 μg/g, 0.114 μg/g, 0.110 μg/g, and 0.147 μg/g, and the GIC<sub>50</sub> values were 0.014 μg/g, 0.073 μg/g, 0.027 μg/g, and 0.026 μg/g, respectively. Determination of the expression content of the insecticidal protein in different tissues of Bt-(Cry1Ab+Vip3Aa) maize shows that the total Bt protein content in different tissues was in the following order: stalk > bract > cob > kernel. However, the bioassay results show that the mortalities of <i>P. gularis</i> feeding on Bt-(Cry1Ab+Vip3Aa) maize in different tissues at different growth stages were all above 93.00%. The field trial indicates that the occurrence density of larvae and plant damage rate for conventional maize were 422.10 individuals/100 plants and 94.40%, respectively, whereas no larvae were found on Bt-(Cry1Ab+Vip3Aa) maize. In summary, this study implies that Bt-(Cry1Ab+Vip3Aa) maize has a high potential for control of <i>P. gularis</i>, providing a new technical measure for the management of the pest. |
| first_indexed | 2024-03-07T22:12:29Z |
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| spelling | doaj.art-d85aea6376454c7f8c9435ff0708bdaa2024-02-23T15:36:34ZengMDPI AGToxins2072-66512024-02-011629210.3390/toxins16020092Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize on Storage Pest <i>Paralipsa gularis</i> (Zeller)Shuang Chen0Wenhui Wang1Guodong Kang2Xianming Yang3Kongming Wu4College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, ChinaInstitute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China<i>Paralipsa gularis</i> (Zeller) is a storage pest; however, in recent years it has evolved into a considerable maize pest during the late growth stage in the border region between China and other Southeast Asian countries. Bt transgenic insect-resistant maize is an effective measure in controlling a wide range of lepidopteran pests, but there is a lack of research on the toxic effects of storage pests. We tested the toxicity of Bt-Cry1Ab, Vip3Aa, and their complex proteins against <i>P. gularis</i> via bioassay and investigated the efficiency of Bt-(Cry1Ab+Vip3Aa) maize in controlling <i>P. gularis</i> during the late growth stage of maize in the period 2022–2023. The bioassay results show that the susceptibilities of <i>P. gularis</i> to the two Bt proteins and their complex proteins were significantly different. The LC<sub>50</sub> values of DBNCry1Ab (“DBN9936” event), DBNVip3Aa (“DBN9501” event), DBN Cry1Ab+Vip3Aa (“DBN3601T” event), and Syngenta Cry1Ab+Vip3Aa (“Bt11” event × “MIR162” event) were 0.038 μg/g, 0.114 μg/g, 0.110 μg/g, and 0.147 μg/g, and the GIC<sub>50</sub> values were 0.014 μg/g, 0.073 μg/g, 0.027 μg/g, and 0.026 μg/g, respectively. Determination of the expression content of the insecticidal protein in different tissues of Bt-(Cry1Ab+Vip3Aa) maize shows that the total Bt protein content in different tissues was in the following order: stalk > bract > cob > kernel. However, the bioassay results show that the mortalities of <i>P. gularis</i> feeding on Bt-(Cry1Ab+Vip3Aa) maize in different tissues at different growth stages were all above 93.00%. The field trial indicates that the occurrence density of larvae and plant damage rate for conventional maize were 422.10 individuals/100 plants and 94.40%, respectively, whereas no larvae were found on Bt-(Cry1Ab+Vip3Aa) maize. In summary, this study implies that Bt-(Cry1Ab+Vip3Aa) maize has a high potential for control of <i>P. gularis</i>, providing a new technical measure for the management of the pest.https://www.mdpi.com/2072-6651/16/2/92<i>Paralipsa gularis</i> (Zeller)Cry1Ab+Vip3Aa maizeBt insecticidal proteins |
| spellingShingle | Shuang Chen Wenhui Wang Guodong Kang Xianming Yang Kongming Wu Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize on Storage Pest <i>Paralipsa gularis</i> (Zeller) Toxins <i>Paralipsa gularis</i> (Zeller) Cry1Ab+Vip3Aa maize Bt insecticidal proteins |
| title | Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize on Storage Pest <i>Paralipsa gularis</i> (Zeller) |
| title_full | Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize on Storage Pest <i>Paralipsa gularis</i> (Zeller) |
| title_fullStr | Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize on Storage Pest <i>Paralipsa gularis</i> (Zeller) |
| title_full_unstemmed | Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize on Storage Pest <i>Paralipsa gularis</i> (Zeller) |
| title_short | Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize on Storage Pest <i>Paralipsa gularis</i> (Zeller) |
| title_sort | toxic effects of bt cry1ab vip3aa maize on storage pest i paralipsa gularis i zeller |
| topic | <i>Paralipsa gularis</i> (Zeller) Cry1Ab+Vip3Aa maize Bt insecticidal proteins |
| url | https://www.mdpi.com/2072-6651/16/2/92 |
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