CDKN2AIP is critical for spermiogenesis and germ cell development
Abstract Background As a member of RNA-binding protein, CDKN2AIP has been shown to play a critical role in stem cell pluripotency and somatic differentiation. Recent studies indicate that Cdkn2aip is essential for spermatogonial self-renewal and proliferation through the activating Wnt-signaling pat...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | Cell & Bioscience |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13578-022-00861-z |
| _version_ | 1817998993499619328 |
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| author | Yuming Cao Qi Sun Zhenlie Chen Jing Lu Ting Geng Ling Ma Yuanzhen Zhang |
| author_facet | Yuming Cao Qi Sun Zhenlie Chen Jing Lu Ting Geng Ling Ma Yuanzhen Zhang |
| author_sort | Yuming Cao |
| collection | DOAJ |
| description | Abstract Background As a member of RNA-binding protein, CDKN2AIP has been shown to play a critical role in stem cell pluripotency and somatic differentiation. Recent studies indicate that Cdkn2aip is essential for spermatogonial self-renewal and proliferation through the activating Wnt-signaling pathway. However, the mechanisms of how Cdkn2aip regulate spermatogenesis is poorly characterized. Results We discovered that the CDKN2AIP was expressed in spermatocyte as well as spermatids and participated in spermiogenesis. Cdkn2aip −/− mice exhibited multiple sperm head defects accompanied by age dependent germ cell loss that might be result of protamine replacement failure and impaired SUN1 expression. Loss of Cdkn2aip expression in male mice resulted in synapsis failure in 19% of all spermatocytes and increased apoptosis due to damaged DNA double-strand break (DSB) repair and crossover formation. In vitro, knockdown of Cdkn2aip was associated with extended S phase, increased DNA damage and apoptosis. Conclusions Our findings not only identified the importance of CDKN2AIP in spermiogenesis and germ cell development, but also provided insight upon the driving mechanism. |
| first_indexed | 2024-04-14T03:01:55Z |
| format | Article |
| id | doaj.art-d85ce58c771648048e8e716d1cf645b7 |
| institution | Directory Open Access Journal |
| issn | 2045-3701 |
| language | English |
| last_indexed | 2024-04-14T03:01:55Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell & Bioscience |
| spelling | doaj.art-d85ce58c771648048e8e716d1cf645b72022-12-22T02:15:52ZengBMCCell & Bioscience2045-37012022-08-0112111810.1186/s13578-022-00861-zCDKN2AIP is critical for spermiogenesis and germ cell developmentYuming Cao0Qi Sun1Zhenlie Chen2Jing Lu3Ting Geng4Ling Ma5Yuanzhen Zhang6Reproductive Medicine Center, Zhongnan Hospital of Wuhan UniversityHubei Provincial Key Laboratory of Developmentally Originated Disease, School of Basic Medical Sciences, Wuhan UniversityReproductive Medicine Center, Zhongnan Hospital of Wuhan UniversityDepartment of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan UniversityReproductive Medicine Center, Zhongnan Hospital of Wuhan UniversityReproductive Medicine Center, Zhongnan Hospital of Wuhan UniversityReproductive Medicine Center, Zhongnan Hospital of Wuhan UniversityAbstract Background As a member of RNA-binding protein, CDKN2AIP has been shown to play a critical role in stem cell pluripotency and somatic differentiation. Recent studies indicate that Cdkn2aip is essential for spermatogonial self-renewal and proliferation through the activating Wnt-signaling pathway. However, the mechanisms of how Cdkn2aip regulate spermatogenesis is poorly characterized. Results We discovered that the CDKN2AIP was expressed in spermatocyte as well as spermatids and participated in spermiogenesis. Cdkn2aip −/− mice exhibited multiple sperm head defects accompanied by age dependent germ cell loss that might be result of protamine replacement failure and impaired SUN1 expression. Loss of Cdkn2aip expression in male mice resulted in synapsis failure in 19% of all spermatocytes and increased apoptosis due to damaged DNA double-strand break (DSB) repair and crossover formation. In vitro, knockdown of Cdkn2aip was associated with extended S phase, increased DNA damage and apoptosis. Conclusions Our findings not only identified the importance of CDKN2AIP in spermiogenesis and germ cell development, but also provided insight upon the driving mechanism.https://doi.org/10.1186/s13578-022-00861-zSpermiogenesisGerm cell developmentDNA double-strand break repairProtamine replacementMale infertility |
| spellingShingle | Yuming Cao Qi Sun Zhenlie Chen Jing Lu Ting Geng Ling Ma Yuanzhen Zhang CDKN2AIP is critical for spermiogenesis and germ cell development Cell & Bioscience Spermiogenesis Germ cell development DNA double-strand break repair Protamine replacement Male infertility |
| title | CDKN2AIP is critical for spermiogenesis and germ cell development |
| title_full | CDKN2AIP is critical for spermiogenesis and germ cell development |
| title_fullStr | CDKN2AIP is critical for spermiogenesis and germ cell development |
| title_full_unstemmed | CDKN2AIP is critical for spermiogenesis and germ cell development |
| title_short | CDKN2AIP is critical for spermiogenesis and germ cell development |
| title_sort | cdkn2aip is critical for spermiogenesis and germ cell development |
| topic | Spermiogenesis Germ cell development DNA double-strand break repair Protamine replacement Male infertility |
| url | https://doi.org/10.1186/s13578-022-00861-z |
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