OAF is a DAF-like gene that controls ovule development in plants
Abstract We previously found that the RING-type E3 ligase DEFECTIVE IN ANTHER DEHISCENCE1- (DAD1-) Activating Factor (DAF) controls anther dehiscence by activating the jasmonate biosynthetic pathway in Arabidopsis. Here, we show that in Arabidopsis, the DAF ancestor was duplicated into three genes (...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-05-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-023-04864-5 |
| _version_ | 1797827405266550784 |
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| author | Ya-Chun Li Jhe-Yi Lin Wei-Han Hsu Chen-Ting Kung Shu-Yu Dai Jun-Yi Yang Choon-Meng Tan Chang-Hsien Yang |
| author_facet | Ya-Chun Li Jhe-Yi Lin Wei-Han Hsu Chen-Ting Kung Shu-Yu Dai Jun-Yi Yang Choon-Meng Tan Chang-Hsien Yang |
| author_sort | Ya-Chun Li |
| collection | DOAJ |
| description | Abstract We previously found that the RING-type E3 ligase DEFECTIVE IN ANTHER DEHISCENCE1- (DAD1-) Activating Factor (DAF) controls anther dehiscence by activating the jasmonate biosynthetic pathway in Arabidopsis. Here, we show that in Arabidopsis, the DAF ancestor was duplicated into three genes (DAF, Ovule Activating Factor (OAF), DAFL2), which evolved divergent partial functions from their ancestor through subfunctionalization. In this case, DAF-DAD1-JA signaling regulates anther dehiscence, whereas OAF controls ovule development by negatively regulating cinnamyl alcohol dehydrogenase 9 (CAD9) activity and being negatively regulated by miR847 itself in Arabidopsis. Downregulation of OAF or upregulation of CAD9 and miR847 caused similar abortion of ovule formation due to precocious ovule lignification in transgenic Arabidopsis. Interestingly, only one DAF-like gene, PaOAF, exists in the monocot orchids, which has likely evolved through nonfunctionalization and maintains a conserved function as Arabidopsis OAF in regulating ovule development since defective ovules were observed in the virus-induced gene silencing (VIGS) PaOAF Phalaenopsis orchids. The absence of the DAF ortholog and its function in orchids is likely due to the evolution of stamens to a unique pollinium structure that lacks the feature of anther dehiscence. These findings expand the current knowledge underlying the multifunctional evolution and diverse functionalization of duplicate gene pairs within/among plants. |
| first_indexed | 2024-04-09T12:47:46Z |
| format | Article |
| id | doaj.art-d85ed83c71324849a4268ea91833e351 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-04-09T12:47:46Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-d85ed83c71324849a4268ea91833e3512023-05-14T11:24:50ZengNature PortfolioCommunications Biology2399-36422023-05-016111510.1038/s42003-023-04864-5OAF is a DAF-like gene that controls ovule development in plantsYa-Chun Li0Jhe-Yi Lin1Wei-Han Hsu2Chen-Ting Kung3Shu-Yu Dai4Jun-Yi Yang5Choon-Meng Tan6Chang-Hsien Yang7Institute of Biotechnology, National Chung Hsing UniversityInstitute of Biotechnology, National Chung Hsing UniversityInstitute of Biotechnology, National Chung Hsing UniversityInstitute of Biotechnology, National Chung Hsing UniversityInstitute of Biotechnology, National Chung Hsing UniversityAdvanced Plant and Food Crop Biotechnology Center, National Chung Hsing UniversityInstitute of Biochemistry, National Chung Hsing UniversityInstitute of Biotechnology, National Chung Hsing UniversityAbstract We previously found that the RING-type E3 ligase DEFECTIVE IN ANTHER DEHISCENCE1- (DAD1-) Activating Factor (DAF) controls anther dehiscence by activating the jasmonate biosynthetic pathway in Arabidopsis. Here, we show that in Arabidopsis, the DAF ancestor was duplicated into three genes (DAF, Ovule Activating Factor (OAF), DAFL2), which evolved divergent partial functions from their ancestor through subfunctionalization. In this case, DAF-DAD1-JA signaling regulates anther dehiscence, whereas OAF controls ovule development by negatively regulating cinnamyl alcohol dehydrogenase 9 (CAD9) activity and being negatively regulated by miR847 itself in Arabidopsis. Downregulation of OAF or upregulation of CAD9 and miR847 caused similar abortion of ovule formation due to precocious ovule lignification in transgenic Arabidopsis. Interestingly, only one DAF-like gene, PaOAF, exists in the monocot orchids, which has likely evolved through nonfunctionalization and maintains a conserved function as Arabidopsis OAF in regulating ovule development since defective ovules were observed in the virus-induced gene silencing (VIGS) PaOAF Phalaenopsis orchids. The absence of the DAF ortholog and its function in orchids is likely due to the evolution of stamens to a unique pollinium structure that lacks the feature of anther dehiscence. These findings expand the current knowledge underlying the multifunctional evolution and diverse functionalization of duplicate gene pairs within/among plants.https://doi.org/10.1038/s42003-023-04864-5 |
| spellingShingle | Ya-Chun Li Jhe-Yi Lin Wei-Han Hsu Chen-Ting Kung Shu-Yu Dai Jun-Yi Yang Choon-Meng Tan Chang-Hsien Yang OAF is a DAF-like gene that controls ovule development in plants Communications Biology |
| title | OAF is a DAF-like gene that controls ovule development in plants |
| title_full | OAF is a DAF-like gene that controls ovule development in plants |
| title_fullStr | OAF is a DAF-like gene that controls ovule development in plants |
| title_full_unstemmed | OAF is a DAF-like gene that controls ovule development in plants |
| title_short | OAF is a DAF-like gene that controls ovule development in plants |
| title_sort | oaf is a daf like gene that controls ovule development in plants |
| url | https://doi.org/10.1038/s42003-023-04864-5 |
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