A novel method to analyze leukocyte rolling behavior <it>in vivo</it>
<p>Leukocyte endothelial cell interaction is a fundamentally important process in many disease states. Current methods to analyze such interactions include the parallel-plate flow chamber and intravital microscopy. Here, we present an improvement of the traditional intravital microscopy that a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2004-01-01
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| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | http://www.biologicalprocedures.com/bpo/arts/1/87/m87.htm |
| _version_ | 1818234932556726272 |
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| author | Dunne Jessica L. Goobic Adam P. Acton Scott T. Ley Klaus |
| author_facet | Dunne Jessica L. Goobic Adam P. Acton Scott T. Ley Klaus |
| author_sort | Dunne Jessica L. |
| collection | DOAJ |
| description | <p>Leukocyte endothelial cell interaction is a fundamentally important process in many disease states. Current methods to analyze such interactions include the parallel-plate flow chamber and intravital microscopy. Here, we present an improvement of the traditional intravital microscopy that allows leukocyte-endothelial cell interaction to be studied from the time the leukocyte makes its initial contact with the endothelium until it adheres to or detaches from the endothelium. The leukocyte is tracked throughout the venular tree with the aid of a motorized stage and the rolling and adhesive behavior is measured off-line. Because this method can involve human error, methods to automate the tracking procedure have been developed. This novel tracking method allows for a more detailed examination of leukocyte-endothelial cell interactions. |
| first_indexed | 2024-12-12T11:45:56Z |
| format | Article |
| id | doaj.art-d85ee6ad04364157bfccd4a3b79606c9 |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-12-12T11:45:56Z |
| publishDate | 2004-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj.art-d85ee6ad04364157bfccd4a3b79606c92022-12-22T00:25:27ZengBMCBiological Procedures Online1480-92222004-01-016117317910.1251/bpo87A novel method to analyze leukocyte rolling behavior <it>in vivo</it>Dunne Jessica L.Goobic Adam P.Acton Scott T.Ley Klaus<p>Leukocyte endothelial cell interaction is a fundamentally important process in many disease states. Current methods to analyze such interactions include the parallel-plate flow chamber and intravital microscopy. Here, we present an improvement of the traditional intravital microscopy that allows leukocyte-endothelial cell interaction to be studied from the time the leukocyte makes its initial contact with the endothelium until it adheres to or detaches from the endothelium. The leukocyte is tracked throughout the venular tree with the aid of a motorized stage and the rolling and adhesive behavior is measured off-line. Because this method can involve human error, methods to automate the tracking procedure have been developed. This novel tracking method allows for a more detailed examination of leukocyte-endothelial cell interactions.http://www.biologicalprocedures.com/bpo/arts/1/87/m87.htmAnimalsCell AdhesionLeukocytesMice, KnockoutTumor Necrosis FactorVenules |
| spellingShingle | Dunne Jessica L. Goobic Adam P. Acton Scott T. Ley Klaus A novel method to analyze leukocyte rolling behavior <it>in vivo</it> Biological Procedures Online Animals Cell Adhesion Leukocytes Mice, Knockout Tumor Necrosis Factor Venules |
| title | A novel method to analyze leukocyte rolling behavior <it>in vivo</it> |
| title_full | A novel method to analyze leukocyte rolling behavior <it>in vivo</it> |
| title_fullStr | A novel method to analyze leukocyte rolling behavior <it>in vivo</it> |
| title_full_unstemmed | A novel method to analyze leukocyte rolling behavior <it>in vivo</it> |
| title_short | A novel method to analyze leukocyte rolling behavior <it>in vivo</it> |
| title_sort | novel method to analyze leukocyte rolling behavior it in vivo it |
| topic | Animals Cell Adhesion Leukocytes Mice, Knockout Tumor Necrosis Factor Venules |
| url | http://www.biologicalprocedures.com/bpo/arts/1/87/m87.htm |
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