Melatonin enhanced low-temperature combined with low-light tolerance of pepper (Capsicum annuum L.) seedlings by regulating root growth, antioxidant defense system, and osmotic adjustment
Melatonin (MT) is an important biologically active hormone that plays a vital role in plant growth and development. In particular, it has been investigated for its roles in abiotic stress management. In this study, pepper seedlings were subjected to low-temperature combined with low-light stress (LL...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.998293/full |
| _version_ | 1811208208819159040 |
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| author | Jing Li Jianming Xie Jihua Yu Jian Lyv Junfeng Zhang Dongxia Ding Nenghui Li Jing Zhang Emily Patience Bakpa Yan Yang Tianhang Niu Feng Gao |
| author_facet | Jing Li Jianming Xie Jihua Yu Jian Lyv Junfeng Zhang Dongxia Ding Nenghui Li Jing Zhang Emily Patience Bakpa Yan Yang Tianhang Niu Feng Gao |
| author_sort | Jing Li |
| collection | DOAJ |
| description | Melatonin (MT) is an important biologically active hormone that plays a vital role in plant growth and development. In particular, it has been investigated for its roles in abiotic stress management. In this study, pepper seedlings were subjected to low-temperature combined with low-light stress (LL) (15/5°C, 100 μmol m-2s-1) prior to a foliar spray of 200mM MT for 168h to investigate the protective role of MT in pepper seedlings. Our results demonstrated that LL stress negatively affected root growth, and accelerated the accumulation of reactive oxygen species (ROS), including H2O2 and O2−, changed the osmolytes contents, and antioxidative system. However, these were reversed by exogenous MT application. MT effectively promoted the root growth as indicated by significant increase in root length, surface area, root volume, tips, forks, and crossings. In addition, MT reduced the burst of ROS and MDA contents induced by LL, enhanced the activities of SOD, CAT, POD, APX, DHAR, and MDHAR resulted by upregulated expressions of CaSOD, CaPOD, CaCAT, CaAPX, CaDHAR, and CaMDHAR, and elevated the contents of AsA and GSH, declined DHA and GSSH contents, which prevented membrane lipid peroxidation and protected plants from oxidative damages under LL stress. Furthermore, seedlings treated with MT released high contents of soluble sugar and soluble protein in leave, which might enhance LL tolerance by maintaining substance biosynthesis and maintaining cellular homeostasis resulted by high levels of osmolytes and carbohydrate in the cytosol. Our current findings confirmed the mitigating potential of MT application for LL stress by promoting pepper root growth, improving antioxidative defense system, ascorbate-glutathione cycle, and osmotic adjustment. |
| first_indexed | 2024-04-12T04:18:19Z |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T04:18:19Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-d85f75c8d3994e91ba16311df2e4bec42022-12-22T03:48:20ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.998293998293Melatonin enhanced low-temperature combined with low-light tolerance of pepper (Capsicum annuum L.) seedlings by regulating root growth, antioxidant defense system, and osmotic adjustmentJing Li0Jianming Xie1Jihua Yu2Jian Lyv3Junfeng Zhang4Dongxia Ding5Nenghui Li6Jing Zhang7Emily Patience Bakpa8Yan Yang9Tianhang Niu10Feng Gao11College of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaInstitution of Vegetable, Gansu Academy of Agricultural Science, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou, ChinaMelatonin (MT) is an important biologically active hormone that plays a vital role in plant growth and development. In particular, it has been investigated for its roles in abiotic stress management. In this study, pepper seedlings were subjected to low-temperature combined with low-light stress (LL) (15/5°C, 100 μmol m-2s-1) prior to a foliar spray of 200mM MT for 168h to investigate the protective role of MT in pepper seedlings. Our results demonstrated that LL stress negatively affected root growth, and accelerated the accumulation of reactive oxygen species (ROS), including H2O2 and O2−, changed the osmolytes contents, and antioxidative system. However, these were reversed by exogenous MT application. MT effectively promoted the root growth as indicated by significant increase in root length, surface area, root volume, tips, forks, and crossings. In addition, MT reduced the burst of ROS and MDA contents induced by LL, enhanced the activities of SOD, CAT, POD, APX, DHAR, and MDHAR resulted by upregulated expressions of CaSOD, CaPOD, CaCAT, CaAPX, CaDHAR, and CaMDHAR, and elevated the contents of AsA and GSH, declined DHA and GSSH contents, which prevented membrane lipid peroxidation and protected plants from oxidative damages under LL stress. Furthermore, seedlings treated with MT released high contents of soluble sugar and soluble protein in leave, which might enhance LL tolerance by maintaining substance biosynthesis and maintaining cellular homeostasis resulted by high levels of osmolytes and carbohydrate in the cytosol. Our current findings confirmed the mitigating potential of MT application for LL stress by promoting pepper root growth, improving antioxidative defense system, ascorbate-glutathione cycle, and osmotic adjustment.https://www.frontiersin.org/articles/10.3389/fpls.2022.998293/fullpeppermelatoninroot morphologyreactive oxygen speciesantioxidant defense systemascorbate-glutathione cycle |
| spellingShingle | Jing Li Jianming Xie Jihua Yu Jian Lyv Junfeng Zhang Dongxia Ding Nenghui Li Jing Zhang Emily Patience Bakpa Yan Yang Tianhang Niu Feng Gao Melatonin enhanced low-temperature combined with low-light tolerance of pepper (Capsicum annuum L.) seedlings by regulating root growth, antioxidant defense system, and osmotic adjustment Frontiers in Plant Science pepper melatonin root morphology reactive oxygen species antioxidant defense system ascorbate-glutathione cycle |
| title | Melatonin enhanced low-temperature combined with low-light tolerance of pepper (Capsicum annuum L.) seedlings by regulating root growth, antioxidant defense system, and osmotic adjustment |
| title_full | Melatonin enhanced low-temperature combined with low-light tolerance of pepper (Capsicum annuum L.) seedlings by regulating root growth, antioxidant defense system, and osmotic adjustment |
| title_fullStr | Melatonin enhanced low-temperature combined with low-light tolerance of pepper (Capsicum annuum L.) seedlings by regulating root growth, antioxidant defense system, and osmotic adjustment |
| title_full_unstemmed | Melatonin enhanced low-temperature combined with low-light tolerance of pepper (Capsicum annuum L.) seedlings by regulating root growth, antioxidant defense system, and osmotic adjustment |
| title_short | Melatonin enhanced low-temperature combined with low-light tolerance of pepper (Capsicum annuum L.) seedlings by regulating root growth, antioxidant defense system, and osmotic adjustment |
| title_sort | melatonin enhanced low temperature combined with low light tolerance of pepper capsicum annuum l seedlings by regulating root growth antioxidant defense system and osmotic adjustment |
| topic | pepper melatonin root morphology reactive oxygen species antioxidant defense system ascorbate-glutathione cycle |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.998293/full |
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