Bioinformatic Characterization of Mosquito Viromes within the Eastern United States and Puerto Rico: Discovery of Novel Viruses
Mosquitoes are efficient, militarily relevant vectors of infectious disease pathogens, including many RNA viruses. The vast majority of all viruses are thought to be undiscovered. Accordingly, recent studies have shown that viruses discovered in insects are very divergent from known pathogens and th...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2016-01-01
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| Series: | Evolutionary Bioinformatics |
| Online Access: | https://doi.org/10.4137/EBO.S38518 |
| _version_ | 1829102169622577152 |
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| author | Kenneth G. Frey Tara Biser Theron Hamilton Carlos J. Santos Guillermo Pimentel Vishwesh P. Mokashi Kimberly A. Bishop-Lilly |
| author_facet | Kenneth G. Frey Tara Biser Theron Hamilton Carlos J. Santos Guillermo Pimentel Vishwesh P. Mokashi Kimberly A. Bishop-Lilly |
| author_sort | Kenneth G. Frey |
| collection | DOAJ |
| description | Mosquitoes are efficient, militarily relevant vectors of infectious disease pathogens, including many RNA viruses. The vast majority of all viruses are thought to be undiscovered. Accordingly, recent studies have shown that viruses discovered in insects are very divergent from known pathogens and that many of them lack appropriate reference sequences in the public databases. Given that the majority of viruses are likely still undiscovered, environmental sampling stands to provide much needed reference samples as well as genetic sequences for comparison. In this study, we sought to determine whether samples of mosquitoes collected from different sites (the Caribbean and locations on the US East Coast) could be differentiated using metagenomic analysis of the RNA viral fraction. We report here distinct virome profiles, even from samples collected short distances apart. In addition to profiling the previously known viruses from these samples, we detected a number of viruses that have been previously undiscovered. |
| first_indexed | 2024-12-10T22:56:00Z |
| format | Article |
| id | doaj.art-d860544ef16e43399c65e308aa9ee9ff |
| institution | Directory Open Access Journal |
| issn | 1176-9343 |
| language | English |
| last_indexed | 2024-12-10T22:56:00Z |
| publishDate | 2016-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Evolutionary Bioinformatics |
| spelling | doaj.art-d860544ef16e43399c65e308aa9ee9ff2022-12-22T01:30:16ZengSAGE PublishingEvolutionary Bioinformatics1176-93432016-01-0112s210.4137/EBO.S38518Bioinformatic Characterization of Mosquito Viromes within the Eastern United States and Puerto Rico: Discovery of Novel VirusesKenneth G. Frey0Tara Biser1Theron Hamilton2Carlos J. Santos3Guillermo Pimentel4Vishwesh P. Mokashi5Kimberly A. Bishop-Lilly6Henry M. Jackson Foundation, Bethesda, MD, USA.Hood College, Frederick, MD, USA.Naval Medical Research Center – Frederick, Fort Detrick, MD, USA.University of Puerto Rico, Mayagüez, Puerto Rico.Naval Medical Research Center – Frederick, Fort Detrick, MD, USA.Naval Medical Research Center – Frederick, Fort Detrick, MD, USA.Henry M. Jackson Foundation, Bethesda, MD, USA.Mosquitoes are efficient, militarily relevant vectors of infectious disease pathogens, including many RNA viruses. The vast majority of all viruses are thought to be undiscovered. Accordingly, recent studies have shown that viruses discovered in insects are very divergent from known pathogens and that many of them lack appropriate reference sequences in the public databases. Given that the majority of viruses are likely still undiscovered, environmental sampling stands to provide much needed reference samples as well as genetic sequences for comparison. In this study, we sought to determine whether samples of mosquitoes collected from different sites (the Caribbean and locations on the US East Coast) could be differentiated using metagenomic analysis of the RNA viral fraction. We report here distinct virome profiles, even from samples collected short distances apart. In addition to profiling the previously known viruses from these samples, we detected a number of viruses that have been previously undiscovered.https://doi.org/10.4137/EBO.S38518 |
| spellingShingle | Kenneth G. Frey Tara Biser Theron Hamilton Carlos J. Santos Guillermo Pimentel Vishwesh P. Mokashi Kimberly A. Bishop-Lilly Bioinformatic Characterization of Mosquito Viromes within the Eastern United States and Puerto Rico: Discovery of Novel Viruses Evolutionary Bioinformatics |
| title | Bioinformatic Characterization of Mosquito Viromes within the Eastern United States and Puerto Rico: Discovery of Novel Viruses |
| title_full | Bioinformatic Characterization of Mosquito Viromes within the Eastern United States and Puerto Rico: Discovery of Novel Viruses |
| title_fullStr | Bioinformatic Characterization of Mosquito Viromes within the Eastern United States and Puerto Rico: Discovery of Novel Viruses |
| title_full_unstemmed | Bioinformatic Characterization of Mosquito Viromes within the Eastern United States and Puerto Rico: Discovery of Novel Viruses |
| title_short | Bioinformatic Characterization of Mosquito Viromes within the Eastern United States and Puerto Rico: Discovery of Novel Viruses |
| title_sort | bioinformatic characterization of mosquito viromes within the eastern united states and puerto rico discovery of novel viruses |
| url | https://doi.org/10.4137/EBO.S38518 |
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