Neuroprotective Effects of 7-Geranyloxycinnamic Acid from <i>Melicope lunu ankenda</i> Leaves
Neurodegenerative diseases (NDDs) are chronic conditions that have drawn robust interest from the scientific community. Phytotherapeutic agents are becoming an important source of chemicals for the treatment and management of NDDs. Various secondary metabolites have been isolated from <i>Melic...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/25/16/3724 |
| _version_ | 1797557973036302336 |
|---|---|
| author | Zeinab Abdulwanis Mohamed Enas Mohamed Eliaser Mohammed Sani Jaafaru Norshariza Nordin Costas Ioannides Ahmad Faizal Abdull Razis |
| author_facet | Zeinab Abdulwanis Mohamed Enas Mohamed Eliaser Mohammed Sani Jaafaru Norshariza Nordin Costas Ioannides Ahmad Faizal Abdull Razis |
| author_sort | Zeinab Abdulwanis Mohamed |
| collection | DOAJ |
| description | Neurodegenerative diseases (NDDs) are chronic conditions that have drawn robust interest from the scientific community. Phytotherapeutic agents are becoming an important source of chemicals for the treatment and management of NDDs. Various secondary metabolites have been isolated from <i>Melicope lunu-ankenda</i> plant leaves, including phenolic acid derivatives. However, their neuroprotective activity remains unclear. Thus, the aim of this study is to elucidate the in vitro neuroprotective activity of 7-geranyloxycinnamic acid isolated from <i>Melicope lunu-ankenda</i> leaves. The neuroprotective activity was evaluated in differentiated human neuroblastoma (SH-SY5Y) cells by monitoring cell viability using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Moreover, the potential to impair apoptosis in differentiated cells was investigated employing the Annexin V-FITC assay, acridine orange and propidium iodide (AO/PI) staining, and fluorescence microscopy. Morphological assessment and ultrastructural analysis were performed using scanning and transmission electron microscopy to evaluate the effect of 7-geranyloxycinnamic acid on surface morphology and internal features of the differentiated cells. Pre-treatment of neuronal cells with 7-geranyloxycinnamic acid significantly protected the differentiated SH-SY5Y cells against H<sub>2</sub>O<sub>2</sub>-induced apoptosis. Cytoskeleton and cytoplasmic inclusion were similarly protected by the 7-geranyloxycinnamic acid treatment. The present findings demonstrate the neuroprotective potential of 7-geranyloxycinnamic acid against H<sub>2</sub>O<sub>2</sub>-induced neurotoxicity in neuronal cells, which is an established hallmark of neuronal disorders. |
| first_indexed | 2024-03-10T17:24:48Z |
| format | Article |
| id | doaj.art-d862d4a0d09c4e7e8252ee2a422caf33 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T17:24:48Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d862d4a0d09c4e7e8252ee2a422caf332023-11-20T10:14:29ZengMDPI AGMolecules1420-30492020-08-012516372410.3390/molecules25163724Neuroprotective Effects of 7-Geranyloxycinnamic Acid from <i>Melicope lunu ankenda</i> LeavesZeinab Abdulwanis Mohamed0Enas Mohamed Eliaser1Mohammed Sani Jaafaru2Norshariza Nordin3Costas Ioannides4Ahmad Faizal Abdull Razis5Natural Medicines and Products Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, MalaysiaNatural Medicines and Products Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, MalaysiaDepartment of Biochemistry, Kaduna State University, Main Campus, Kaduna PMB 2339, NigeriaDepartment of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, MalaysiaFaculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UKNatural Medicines and Products Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, MalaysiaNeurodegenerative diseases (NDDs) are chronic conditions that have drawn robust interest from the scientific community. Phytotherapeutic agents are becoming an important source of chemicals for the treatment and management of NDDs. Various secondary metabolites have been isolated from <i>Melicope lunu-ankenda</i> plant leaves, including phenolic acid derivatives. However, their neuroprotective activity remains unclear. Thus, the aim of this study is to elucidate the in vitro neuroprotective activity of 7-geranyloxycinnamic acid isolated from <i>Melicope lunu-ankenda</i> leaves. The neuroprotective activity was evaluated in differentiated human neuroblastoma (SH-SY5Y) cells by monitoring cell viability using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Moreover, the potential to impair apoptosis in differentiated cells was investigated employing the Annexin V-FITC assay, acridine orange and propidium iodide (AO/PI) staining, and fluorescence microscopy. Morphological assessment and ultrastructural analysis were performed using scanning and transmission electron microscopy to evaluate the effect of 7-geranyloxycinnamic acid on surface morphology and internal features of the differentiated cells. Pre-treatment of neuronal cells with 7-geranyloxycinnamic acid significantly protected the differentiated SH-SY5Y cells against H<sub>2</sub>O<sub>2</sub>-induced apoptosis. Cytoskeleton and cytoplasmic inclusion were similarly protected by the 7-geranyloxycinnamic acid treatment. The present findings demonstrate the neuroprotective potential of 7-geranyloxycinnamic acid against H<sub>2</sub>O<sub>2</sub>-induced neurotoxicity in neuronal cells, which is an established hallmark of neuronal disorders.https://www.mdpi.com/1420-3049/25/16/3724<i>Melicope lunu-ankenda</i>7-geranyloxycinnamic acidneuroprotectionanti-apoptosisoxidative stress |
| spellingShingle | Zeinab Abdulwanis Mohamed Enas Mohamed Eliaser Mohammed Sani Jaafaru Norshariza Nordin Costas Ioannides Ahmad Faizal Abdull Razis Neuroprotective Effects of 7-Geranyloxycinnamic Acid from <i>Melicope lunu ankenda</i> Leaves Molecules <i>Melicope lunu-ankenda</i> 7-geranyloxycinnamic acid neuroprotection anti-apoptosis oxidative stress |
| title | Neuroprotective Effects of 7-Geranyloxycinnamic Acid from <i>Melicope lunu ankenda</i> Leaves |
| title_full | Neuroprotective Effects of 7-Geranyloxycinnamic Acid from <i>Melicope lunu ankenda</i> Leaves |
| title_fullStr | Neuroprotective Effects of 7-Geranyloxycinnamic Acid from <i>Melicope lunu ankenda</i> Leaves |
| title_full_unstemmed | Neuroprotective Effects of 7-Geranyloxycinnamic Acid from <i>Melicope lunu ankenda</i> Leaves |
| title_short | Neuroprotective Effects of 7-Geranyloxycinnamic Acid from <i>Melicope lunu ankenda</i> Leaves |
| title_sort | neuroprotective effects of 7 geranyloxycinnamic acid from i melicope lunu ankenda i leaves |
| topic | <i>Melicope lunu-ankenda</i> 7-geranyloxycinnamic acid neuroprotection anti-apoptosis oxidative stress |
| url | https://www.mdpi.com/1420-3049/25/16/3724 |
| work_keys_str_mv | AT zeinababdulwanismohamed neuroprotectiveeffectsof7geranyloxycinnamicacidfromimelicopelunuankendaileaves AT enasmohamedeliaser neuroprotectiveeffectsof7geranyloxycinnamicacidfromimelicopelunuankendaileaves AT mohammedsanijaafaru neuroprotectiveeffectsof7geranyloxycinnamicacidfromimelicopelunuankendaileaves AT norsharizanordin neuroprotectiveeffectsof7geranyloxycinnamicacidfromimelicopelunuankendaileaves AT costasioannides neuroprotectiveeffectsof7geranyloxycinnamicacidfromimelicopelunuankendaileaves AT ahmadfaizalabdullrazis neuroprotectiveeffectsof7geranyloxycinnamicacidfromimelicopelunuankendaileaves |