Second external quality assurance study for the serological diagnosis of hantaviruses in Europe.
Hantaviruses are endemic throughout the world and hosted by rodents and insectivores. Two human zoonoses, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS), are caused by hantaviruses and case fatality rates have reached 12% for HFRS and 50% for HPS in some outbrea...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | http://europepmc.org/articles/PMC3317902?pdf=render |
| _version_ | 1818543218581569536 |
|---|---|
| author | Camille Escadafal Tatjana Avšič-Županc Olli Vapalahti Bo Niklasson Anette Teichmann Matthias Niedrig Oliver Donoso-Mantke |
| author_facet | Camille Escadafal Tatjana Avšič-Županc Olli Vapalahti Bo Niklasson Anette Teichmann Matthias Niedrig Oliver Donoso-Mantke |
| author_sort | Camille Escadafal |
| collection | DOAJ |
| description | Hantaviruses are endemic throughout the world and hosted by rodents and insectivores. Two human zoonoses, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS), are caused by hantaviruses and case fatality rates have reached 12% for HFRS and 50% for HPS in some outbreaks. Symptomatic hantavirus infections in Europe are summarised as HFRS mainly due to Puumala, Dobrava-Belgrade and Saaremaa virus. While HFRS has an overall low incidence in Europe, the number of cases varies from 100 per year in all Eastern and Southern Europe up to 1,000 per year only in Finland. To assess the quality of hantavirus diagnostics, the European Network for the Diagnostics of "Imported" Viral Diseases (ENIVD) organised a first external quality assurance (EQA) in 2002. The purpose of this second EQA study is to collect updated information on the efficiency and accurateness of hantavirus serological methods applied by expert laboratories. A serum panel of 14 samples was sent to 28 participants in Europe of which 27 sent results. Performance in hantavirus diagnosis varied not only on the method used but also on the laboratories and the subclass of antibodies tested. Commercial and in-house assays performed almost equally. Enzyme immunoassays were mainly used but did not show the best performances while immunoblot assays were the less employed and showed overall better performances. IgM antibodies were not detected in 61% of the positive IgM samples and IgM detection was not performed by 7% of the laboratories indicating a risk of overlooking acute infections in patients. Uneven performances using the same method is indicating that there is still a need for improving testing conditions and standardizing protocols. |
| first_indexed | 2024-12-11T22:32:27Z |
| format | Article |
| id | doaj.art-d8658fea1fc846b1b1348b7f22e77748 |
| institution | Directory Open Access Journal |
| issn | 1935-2735 |
| language | English |
| last_indexed | 2024-12-11T22:32:27Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-d8658fea1fc846b1b1348b7f22e777482022-12-22T00:48:05ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27352012-01-0164e160710.1371/journal.pntd.0001607Second external quality assurance study for the serological diagnosis of hantaviruses in Europe.Camille EscadafalTatjana Avšič-ŽupancOlli VapalahtiBo NiklassonAnette TeichmannMatthias NiedrigOliver Donoso-MantkeHantaviruses are endemic throughout the world and hosted by rodents and insectivores. Two human zoonoses, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS), are caused by hantaviruses and case fatality rates have reached 12% for HFRS and 50% for HPS in some outbreaks. Symptomatic hantavirus infections in Europe are summarised as HFRS mainly due to Puumala, Dobrava-Belgrade and Saaremaa virus. While HFRS has an overall low incidence in Europe, the number of cases varies from 100 per year in all Eastern and Southern Europe up to 1,000 per year only in Finland. To assess the quality of hantavirus diagnostics, the European Network for the Diagnostics of "Imported" Viral Diseases (ENIVD) organised a first external quality assurance (EQA) in 2002. The purpose of this second EQA study is to collect updated information on the efficiency and accurateness of hantavirus serological methods applied by expert laboratories. A serum panel of 14 samples was sent to 28 participants in Europe of which 27 sent results. Performance in hantavirus diagnosis varied not only on the method used but also on the laboratories and the subclass of antibodies tested. Commercial and in-house assays performed almost equally. Enzyme immunoassays were mainly used but did not show the best performances while immunoblot assays were the less employed and showed overall better performances. IgM antibodies were not detected in 61% of the positive IgM samples and IgM detection was not performed by 7% of the laboratories indicating a risk of overlooking acute infections in patients. Uneven performances using the same method is indicating that there is still a need for improving testing conditions and standardizing protocols.http://europepmc.org/articles/PMC3317902?pdf=render |
| spellingShingle | Camille Escadafal Tatjana Avšič-Županc Olli Vapalahti Bo Niklasson Anette Teichmann Matthias Niedrig Oliver Donoso-Mantke Second external quality assurance study for the serological diagnosis of hantaviruses in Europe. PLoS Neglected Tropical Diseases |
| title | Second external quality assurance study for the serological diagnosis of hantaviruses in Europe. |
| title_full | Second external quality assurance study for the serological diagnosis of hantaviruses in Europe. |
| title_fullStr | Second external quality assurance study for the serological diagnosis of hantaviruses in Europe. |
| title_full_unstemmed | Second external quality assurance study for the serological diagnosis of hantaviruses in Europe. |
| title_short | Second external quality assurance study for the serological diagnosis of hantaviruses in Europe. |
| title_sort | second external quality assurance study for the serological diagnosis of hantaviruses in europe |
| url | http://europepmc.org/articles/PMC3317902?pdf=render |
| work_keys_str_mv | AT camilleescadafal secondexternalqualityassurancestudyfortheserologicaldiagnosisofhantavirusesineurope AT tatjanaavsiczupanc secondexternalqualityassurancestudyfortheserologicaldiagnosisofhantavirusesineurope AT ollivapalahti secondexternalqualityassurancestudyfortheserologicaldiagnosisofhantavirusesineurope AT boniklasson secondexternalqualityassurancestudyfortheserologicaldiagnosisofhantavirusesineurope AT anetteteichmann secondexternalqualityassurancestudyfortheserologicaldiagnosisofhantavirusesineurope AT matthiasniedrig secondexternalqualityassurancestudyfortheserologicaldiagnosisofhantavirusesineurope AT oliverdonosomantke secondexternalqualityassurancestudyfortheserologicaldiagnosisofhantavirusesineurope |