Delphinidins from Maqui Berry (Aristotelia chilensis) ameliorate the subcellular organelle damage induced by blue light exposure in murine photoreceptor-derived cells
Abstract Background Blue light exposure is known to induce reactive oxygen species (ROS) production and increased endoplasmic reticulum stress, leading to apoptosis of photoreceptors. Maqui berry (Aristotelia chilensis) is a fruit enriched in anthocyanins, known for beneficial biological activities...
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Complementary Medicine and Therapies |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12906-023-04322-z |
| _version_ | 1797363698542575616 |
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| author | Kanta Yamazaki Kodai Ishida Wataru Otsu Aomi Muramatsu Shinsuke Nakamura Wakana Yamada Hideshi Tsusaki Hiroshi Shimoda Hideaki Hara Masamitsu Shimazawa |
| author_facet | Kanta Yamazaki Kodai Ishida Wataru Otsu Aomi Muramatsu Shinsuke Nakamura Wakana Yamada Hideshi Tsusaki Hiroshi Shimoda Hideaki Hara Masamitsu Shimazawa |
| author_sort | Kanta Yamazaki |
| collection | DOAJ |
| description | Abstract Background Blue light exposure is known to induce reactive oxygen species (ROS) production and increased endoplasmic reticulum stress, leading to apoptosis of photoreceptors. Maqui berry (Aristotelia chilensis) is a fruit enriched in anthocyanins, known for beneficial biological activities such as antioxidation. In this study, we investigated the effects of Maqui berry extract (MBE) and its constituents on the subcellular damage induced by blue light irradiation in mouse retina-derived 661W cells. Methods We evaluated the effects of MBE and its main delphinidins, delphinidin 3-O-sambubioside-5-O-glucoside (D3S5G) and delphinidin 3,5-O-diglucoside (D3G5G), on blue light-induced damage on retinal cell line 661W cells. We investigated cell death, the production of ROS, and changes in organelle morphology using fluorescence microscopy. The signaling pathway linked to stress response was evaluated by immunoblotting in the whole cell lysates or nuclear fractions. We also examined the effects of MBE and delphinidins against rotenone-induced mitochondrial dysfunction. Results Blue light-induced cell death, increased intracellular ROS generation and mitochondrial fragmentation, decreased ATP-production coupled respiration, caused lysosomal membrane permeabilization, and increased ATF4 protein level. Treatment with MBE and its main constituents, delphinidin 3-O-sambubioside-5-O-glucoside and delphinidin 3,5-O-diglucoside, prevented these defects. Furthermore, MBE and delphinidins also protected 661W cells from rotenone-induced cell death. Conclusions Maqui berry may be a useful protective agent for photoreceptors against the oxidative damage induced by exposure to blue light. Graphical abstract |
| first_indexed | 2024-03-08T16:24:43Z |
| format | Article |
| id | doaj.art-d865dad8afd8404b82ffe56ad659dd73 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-03-08T16:24:43Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-d865dad8afd8404b82ffe56ad659dd732024-01-07T12:11:04ZengBMCBMC Complementary Medicine and Therapies2662-76712024-01-0124111610.1186/s12906-023-04322-zDelphinidins from Maqui Berry (Aristotelia chilensis) ameliorate the subcellular organelle damage induced by blue light exposure in murine photoreceptor-derived cellsKanta Yamazaki0Kodai Ishida1Wataru Otsu2Aomi Muramatsu3Shinsuke Nakamura4Wakana Yamada5Hideshi Tsusaki6Hiroshi Shimoda7Hideaki Hara8Masamitsu Shimazawa9Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical UniversityDepartment of Biomedical Research Laboratory, Gifu Pharmaceutical UniversityDepartment of Biomedical Research Laboratory, Gifu Pharmaceutical UniversityMolecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical UniversityMolecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical UniversityResearch & Development Division, Oryza Oil & Fat Chemical Co., LtdDepartment of Biomedical Research Laboratory, Gifu Pharmaceutical UniversityResearch & Development Division, Oryza Oil & Fat Chemical Co., LtdMolecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical UniversityMolecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical UniversityAbstract Background Blue light exposure is known to induce reactive oxygen species (ROS) production and increased endoplasmic reticulum stress, leading to apoptosis of photoreceptors. Maqui berry (Aristotelia chilensis) is a fruit enriched in anthocyanins, known for beneficial biological activities such as antioxidation. In this study, we investigated the effects of Maqui berry extract (MBE) and its constituents on the subcellular damage induced by blue light irradiation in mouse retina-derived 661W cells. Methods We evaluated the effects of MBE and its main delphinidins, delphinidin 3-O-sambubioside-5-O-glucoside (D3S5G) and delphinidin 3,5-O-diglucoside (D3G5G), on blue light-induced damage on retinal cell line 661W cells. We investigated cell death, the production of ROS, and changes in organelle morphology using fluorescence microscopy. The signaling pathway linked to stress response was evaluated by immunoblotting in the whole cell lysates or nuclear fractions. We also examined the effects of MBE and delphinidins against rotenone-induced mitochondrial dysfunction. Results Blue light-induced cell death, increased intracellular ROS generation and mitochondrial fragmentation, decreased ATP-production coupled respiration, caused lysosomal membrane permeabilization, and increased ATF4 protein level. Treatment with MBE and its main constituents, delphinidin 3-O-sambubioside-5-O-glucoside and delphinidin 3,5-O-diglucoside, prevented these defects. Furthermore, MBE and delphinidins also protected 661W cells from rotenone-induced cell death. Conclusions Maqui berry may be a useful protective agent for photoreceptors against the oxidative damage induced by exposure to blue light. Graphical abstracthttps://doi.org/10.1186/s12906-023-04322-zAnthocyaninIntegrated stress responseLight-emitting diodeMitochondriaOxidative stressPhotoreceptor |
| spellingShingle | Kanta Yamazaki Kodai Ishida Wataru Otsu Aomi Muramatsu Shinsuke Nakamura Wakana Yamada Hideshi Tsusaki Hiroshi Shimoda Hideaki Hara Masamitsu Shimazawa Delphinidins from Maqui Berry (Aristotelia chilensis) ameliorate the subcellular organelle damage induced by blue light exposure in murine photoreceptor-derived cells BMC Complementary Medicine and Therapies Anthocyanin Integrated stress response Light-emitting diode Mitochondria Oxidative stress Photoreceptor |
| title | Delphinidins from Maqui Berry (Aristotelia chilensis) ameliorate the subcellular organelle damage induced by blue light exposure in murine photoreceptor-derived cells |
| title_full | Delphinidins from Maqui Berry (Aristotelia chilensis) ameliorate the subcellular organelle damage induced by blue light exposure in murine photoreceptor-derived cells |
| title_fullStr | Delphinidins from Maqui Berry (Aristotelia chilensis) ameliorate the subcellular organelle damage induced by blue light exposure in murine photoreceptor-derived cells |
| title_full_unstemmed | Delphinidins from Maqui Berry (Aristotelia chilensis) ameliorate the subcellular organelle damage induced by blue light exposure in murine photoreceptor-derived cells |
| title_short | Delphinidins from Maqui Berry (Aristotelia chilensis) ameliorate the subcellular organelle damage induced by blue light exposure in murine photoreceptor-derived cells |
| title_sort | delphinidins from maqui berry aristotelia chilensis ameliorate the subcellular organelle damage induced by blue light exposure in murine photoreceptor derived cells |
| topic | Anthocyanin Integrated stress response Light-emitting diode Mitochondria Oxidative stress Photoreceptor |
| url | https://doi.org/10.1186/s12906-023-04322-z |
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