Cytochrome P450 Monooxygenases <i>CYP6AY3</i> and <i>CYP6CW1</i> Regulate Rice Black-Streaked Dwarf Virus Replication in <i>Laodelphax striatellus</i> (Fallén)
The small brown planthopper, <i>Laodelphax striatellus</i> (Fallén), is an important agricultural pest that causes significant losses by sucking and transmitting multiple plant viruses, such as rice black-streaked dwarf virus (RBSDV). Insecticides are commonly used to control planthopper...
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MDPI AG
2021-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/8/1576 |
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| author | Jian-Hua Zhang Ming Zhao Yi-Jun Zhou Qiu-Fang Xu Yuan-Xue Yang |
| author_facet | Jian-Hua Zhang Ming Zhao Yi-Jun Zhou Qiu-Fang Xu Yuan-Xue Yang |
| author_sort | Jian-Hua Zhang |
| collection | DOAJ |
| description | The small brown planthopper, <i>Laodelphax striatellus</i> (Fallén), is an important agricultural pest that causes significant losses by sucking and transmitting multiple plant viruses, such as rice black-streaked dwarf virus (RBSDV). Insecticides are commonly used to control planthoppers and cause the induction or overexpression of cytochrome P450 monooxygenases (P450s) from the CYP3 and CYP4 clades after insecticide application. However, little is known about the roles of insecticides and P450s in the regulation of viral replication in insects. In this study, RBSDV-infected <i>L. striatellus</i> were injected with imidacloprid, deltamethrin, pymetrozine, and buprofezin, respectively. The insecticide treatments caused a significant decrease in RBSDV abundance in <i>L. striatellus</i>. Treatment of piperonyl butoxide (PBO), an effective inhibitor of P450s, significantly increased the RBSDV abundance in <i>L. striatellus</i>. Fourteen P450 candidate genes in the CYP3 clade and 21 in the CYP4 clade were systematically identified in <i>L. striatellus</i>, and their expression patterns were analyzed under RBSDV infection, in different tissues, and at different developmental stages. Among the thirty-five P450 genes, the expression level of <i>CYP6CW1</i> was the highest, while <i>CYP6AY3</i> was the lowest after RBSDV infection. Knockdown of <i>CYP6CW1</i> and <i>CYP6AY3</i> significantly increased the virus abundance and promoted virus replication in <i>L. striatellus</i>. Overall, our data reveal that <i>CYP6CW1</i> and <i>CYP6AY3</i> play a critical role in the regulation of virus replication in <i>L.</i><i>striatellus</i>. |
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| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T08:19:00Z |
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| series | Viruses |
| spelling | doaj.art-d8660a97aef8488ea5668968b0c433972023-11-22T10:11:33ZengMDPI AGViruses1999-49152021-08-01138157610.3390/v13081576Cytochrome P450 Monooxygenases <i>CYP6AY3</i> and <i>CYP6CW1</i> Regulate Rice Black-Streaked Dwarf Virus Replication in <i>Laodelphax striatellus</i> (Fallén)Jian-Hua Zhang0Ming Zhao1Yi-Jun Zhou2Qiu-Fang Xu3Yuan-Xue Yang4Key Laboratory of Food Quality and Safety of Jiangsu Province, State Key Laboratory Breeding Base, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaKey Laboratory of Food Quality and Safety of Jiangsu Province, State Key Laboratory Breeding Base, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaKey Laboratory of Food Quality and Safety of Jiangsu Province, State Key Laboratory Breeding Base, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaThe small brown planthopper, <i>Laodelphax striatellus</i> (Fallén), is an important agricultural pest that causes significant losses by sucking and transmitting multiple plant viruses, such as rice black-streaked dwarf virus (RBSDV). Insecticides are commonly used to control planthoppers and cause the induction or overexpression of cytochrome P450 monooxygenases (P450s) from the CYP3 and CYP4 clades after insecticide application. However, little is known about the roles of insecticides and P450s in the regulation of viral replication in insects. In this study, RBSDV-infected <i>L. striatellus</i> were injected with imidacloprid, deltamethrin, pymetrozine, and buprofezin, respectively. The insecticide treatments caused a significant decrease in RBSDV abundance in <i>L. striatellus</i>. Treatment of piperonyl butoxide (PBO), an effective inhibitor of P450s, significantly increased the RBSDV abundance in <i>L. striatellus</i>. Fourteen P450 candidate genes in the CYP3 clade and 21 in the CYP4 clade were systematically identified in <i>L. striatellus</i>, and their expression patterns were analyzed under RBSDV infection, in different tissues, and at different developmental stages. Among the thirty-five P450 genes, the expression level of <i>CYP6CW1</i> was the highest, while <i>CYP6AY3</i> was the lowest after RBSDV infection. Knockdown of <i>CYP6CW1</i> and <i>CYP6AY3</i> significantly increased the virus abundance and promoted virus replication in <i>L. striatellus</i>. Overall, our data reveal that <i>CYP6CW1</i> and <i>CYP6AY3</i> play a critical role in the regulation of virus replication in <i>L.</i><i>striatellus</i>.https://www.mdpi.com/1999-4915/13/8/1576insecticides<i>Laodelphax striatellus</i>rice black-streaked dwarf virusP450s<i>CYP6CW1</i><i>CYP6AY3</i> |
| spellingShingle | Jian-Hua Zhang Ming Zhao Yi-Jun Zhou Qiu-Fang Xu Yuan-Xue Yang Cytochrome P450 Monooxygenases <i>CYP6AY3</i> and <i>CYP6CW1</i> Regulate Rice Black-Streaked Dwarf Virus Replication in <i>Laodelphax striatellus</i> (Fallén) Viruses insecticides <i>Laodelphax striatellus</i> rice black-streaked dwarf virus P450s <i>CYP6CW1</i> <i>CYP6AY3</i> |
| title | Cytochrome P450 Monooxygenases <i>CYP6AY3</i> and <i>CYP6CW1</i> Regulate Rice Black-Streaked Dwarf Virus Replication in <i>Laodelphax striatellus</i> (Fallén) |
| title_full | Cytochrome P450 Monooxygenases <i>CYP6AY3</i> and <i>CYP6CW1</i> Regulate Rice Black-Streaked Dwarf Virus Replication in <i>Laodelphax striatellus</i> (Fallén) |
| title_fullStr | Cytochrome P450 Monooxygenases <i>CYP6AY3</i> and <i>CYP6CW1</i> Regulate Rice Black-Streaked Dwarf Virus Replication in <i>Laodelphax striatellus</i> (Fallén) |
| title_full_unstemmed | Cytochrome P450 Monooxygenases <i>CYP6AY3</i> and <i>CYP6CW1</i> Regulate Rice Black-Streaked Dwarf Virus Replication in <i>Laodelphax striatellus</i> (Fallén) |
| title_short | Cytochrome P450 Monooxygenases <i>CYP6AY3</i> and <i>CYP6CW1</i> Regulate Rice Black-Streaked Dwarf Virus Replication in <i>Laodelphax striatellus</i> (Fallén) |
| title_sort | cytochrome p450 monooxygenases i cyp6ay3 i and i cyp6cw1 i regulate rice black streaked dwarf virus replication in i laodelphax striatellus i fallen |
| topic | insecticides <i>Laodelphax striatellus</i> rice black-streaked dwarf virus P450s <i>CYP6CW1</i> <i>CYP6AY3</i> |
| url | https://www.mdpi.com/1999-4915/13/8/1576 |
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