A single dose of lipopolysaccharide elicits autofluorescence in the mouse brain
One hallmark of aging is autofluorescence (AF) in the brain. However, the underlying mechanism for inducing AF remains unknown. This study aims to determine the cause(s) of this phenomenon. The endogenous expression pattern of AF in mice was examined at differing ages. Intraperitoneal injection of a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Aging Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnagi.2023.1126273/full |
| _version_ | 1827986838033268736 |
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| author | Yanzhuo Yang Qingting Yu Bin Li Shijia Li Zuisu Yang Falei Yuan Zhongliang Liu |
| author_facet | Yanzhuo Yang Qingting Yu Bin Li Shijia Li Zuisu Yang Falei Yuan Zhongliang Liu |
| author_sort | Yanzhuo Yang |
| collection | DOAJ |
| description | One hallmark of aging is autofluorescence (AF) in the brain. However, the underlying mechanism for inducing AF remains unknown. This study aims to determine the cause(s) of this phenomenon. The endogenous expression pattern of AF in mice was examined at differing ages. Intraperitoneal injection of a single dose of lipopolysaccharide (LPS) was performed to induce AF. Copper sulfate was applied to remove AF to allow for further immunofluorescence staining. AF appeared in the mouse brain as early as 3 months of age. In the cortex, AF occurs in the lysosomes of microglia, astrocytes, endothelial cells, and oligodendrocyte lineage cells and its prevalence increases with age. Interestingly, AF never occurs in the pericytes of young or aged brains. LPS administration resulted in a rapid and marked induction of brain AF, similar to the normal aging process. Finally, age-related and induced AF can be eliminated by low concentrations of copper sulfate solution. This pre-treatment is safe for aging and lineage tracing studies. These findings depict that AF in the brain could be associated with the innate immune response against Gram-negative bacteria infection. |
| first_indexed | 2024-04-09T23:37:42Z |
| format | Article |
| id | doaj.art-d869406ee06a49b7871cfe8c32c48119 |
| institution | Directory Open Access Journal |
| issn | 1663-4365 |
| language | English |
| last_indexed | 2024-04-09T23:37:42Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Aging Neuroscience |
| spelling | doaj.art-d869406ee06a49b7871cfe8c32c481192023-03-20T04:56:54ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652023-03-011510.3389/fnagi.2023.11262731126273A single dose of lipopolysaccharide elicits autofluorescence in the mouse brainYanzhuo Yang0Qingting Yu1Bin Li2Shijia Li3Zuisu Yang4Falei Yuan5Zhongliang Liu6Department of Pharmacy, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, ChinaDepartment of Pharmacy, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, ChinaZhoushan Institute for Food and Drug Control, Zhoushan, ChinaDepartment of Pharmacy, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, ChinaDepartment of Pharmacy, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, ChinaDepartment of Pharmacy, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, ChinaDepartment of Oncology, Zhoushan Hospital of Traditional Chinese Medicine, Zhoushan, ChinaOne hallmark of aging is autofluorescence (AF) in the brain. However, the underlying mechanism for inducing AF remains unknown. This study aims to determine the cause(s) of this phenomenon. The endogenous expression pattern of AF in mice was examined at differing ages. Intraperitoneal injection of a single dose of lipopolysaccharide (LPS) was performed to induce AF. Copper sulfate was applied to remove AF to allow for further immunofluorescence staining. AF appeared in the mouse brain as early as 3 months of age. In the cortex, AF occurs in the lysosomes of microglia, astrocytes, endothelial cells, and oligodendrocyte lineage cells and its prevalence increases with age. Interestingly, AF never occurs in the pericytes of young or aged brains. LPS administration resulted in a rapid and marked induction of brain AF, similar to the normal aging process. Finally, age-related and induced AF can be eliminated by low concentrations of copper sulfate solution. This pre-treatment is safe for aging and lineage tracing studies. These findings depict that AF in the brain could be associated with the innate immune response against Gram-negative bacteria infection.https://www.frontiersin.org/articles/10.3389/fnagi.2023.1126273/fullautofluorescenceagingneuronlipopolysaccharidelipofuscin |
| spellingShingle | Yanzhuo Yang Qingting Yu Bin Li Shijia Li Zuisu Yang Falei Yuan Zhongliang Liu A single dose of lipopolysaccharide elicits autofluorescence in the mouse brain Frontiers in Aging Neuroscience autofluorescence aging neuron lipopolysaccharide lipofuscin |
| title | A single dose of lipopolysaccharide elicits autofluorescence in the mouse brain |
| title_full | A single dose of lipopolysaccharide elicits autofluorescence in the mouse brain |
| title_fullStr | A single dose of lipopolysaccharide elicits autofluorescence in the mouse brain |
| title_full_unstemmed | A single dose of lipopolysaccharide elicits autofluorescence in the mouse brain |
| title_short | A single dose of lipopolysaccharide elicits autofluorescence in the mouse brain |
| title_sort | single dose of lipopolysaccharide elicits autofluorescence in the mouse brain |
| topic | autofluorescence aging neuron lipopolysaccharide lipofuscin |
| url | https://www.frontiersin.org/articles/10.3389/fnagi.2023.1126273/full |
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