Selection and Optimization of a Bioink Based on PANC-1- Plasma/Alginate/Methylcellulose for Pancreatic Tumour Modelling
3D bioprinting involves using bioinks that combine biological and synthetic materials. The selection of the most appropriate cell-material combination for a specific application is complex, and there is a lack of consensus on the optimal conditions required. Plasma-loaded alginate and alginate/methy...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/15/3196 |
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| author | Cristina Banda Sánchez Nieves Cubo Mateo Laura Saldaña Alba Valdivieso Julie Earl Itziar González Gómez Luis M. Rodríguez-Lorenzo |
| author_facet | Cristina Banda Sánchez Nieves Cubo Mateo Laura Saldaña Alba Valdivieso Julie Earl Itziar González Gómez Luis M. Rodríguez-Lorenzo |
| author_sort | Cristina Banda Sánchez |
| collection | DOAJ |
| description | 3D bioprinting involves using bioinks that combine biological and synthetic materials. The selection of the most appropriate cell-material combination for a specific application is complex, and there is a lack of consensus on the optimal conditions required. Plasma-loaded alginate and alginate/methylcellulose (Alg/MC) inks were chosen to study their viscoelastic behaviour, degree of recovery, gelation kinetics, and cell survival after printing. Selected inks showed a shear thinning behavior from shear rates as low as 0.2 s<sup>−1,</sup> and the ink composed of 3% <i>w/v</i> SA and 9% <i>w/v</i> MC was the only one showing a successful stacking and 96% recovery capacity. A 0.5 × 10<sup>6</sup> PANC-1 cell-laden bioink was extruded with an Inkredible 3D printer (Cellink) through a D = 410 μm tip conical nozzle into 6-well culture plates. Cylindrical constructs were printed and crosslinked with CaCl<sub>2</sub>. Bioinks suffered a 1.845 Pa maximum pressure at the tip that was not deleterious for cellular viability. Cell aggregates can be appreciated for the cut total length observed in confocal microscopy, indicating a good proliferation rate at different heights of the construct, and suggesting the viability of the selected bioink PANC-1/P-Alg<sub>3</sub>/MC<sub>9</sub> for building up three-dimensional bioprinted pancreatic tumor constructs. |
| first_indexed | 2024-03-11T00:18:49Z |
| format | Article |
| id | doaj.art-d8700457892e467b8cabb84062382dda |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-11T00:18:49Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-d8700457892e467b8cabb84062382dda2023-11-18T23:27:52ZengMDPI AGPolymers2073-43602023-07-011515319610.3390/polym15153196Selection and Optimization of a Bioink Based on PANC-1- Plasma/Alginate/Methylcellulose for Pancreatic Tumour ModellingCristina Banda Sánchez0Nieves Cubo Mateo1Laura Saldaña2Alba Valdivieso3Julie Earl4Itziar González Gómez5Luis M. Rodríguez-Lorenzo6Institute of Science and Technology of Polymers (ICTP-CSIC), 28006 Madrid, SpainNebrija Research Group ARIES, Higher Polytechnic School, Antonio de Nebrija University, 28015 Madrid, SpainIdiPAZ, Hospital Universitario La Paz, 28046 Madrid, SpainInstitute for Physical and Information Technologies (ITEFI-CSIC), Sensors and Ultrasonic Systems, 28006 Madrid, SpainRamón y Cajal Health Research Institute (IRYCIS), Molecular Epidemiology and Predictive Tumour Markers, 28034 Madrid, SpainInstitute for Physical and Information Technologies (ITEFI-CSIC), Sensors and Ultrasonic Systems, 28006 Madrid, SpainInstitute of Science and Technology of Polymers (ICTP-CSIC), 28006 Madrid, Spain3D bioprinting involves using bioinks that combine biological and synthetic materials. The selection of the most appropriate cell-material combination for a specific application is complex, and there is a lack of consensus on the optimal conditions required. Plasma-loaded alginate and alginate/methylcellulose (Alg/MC) inks were chosen to study their viscoelastic behaviour, degree of recovery, gelation kinetics, and cell survival after printing. Selected inks showed a shear thinning behavior from shear rates as low as 0.2 s<sup>−1,</sup> and the ink composed of 3% <i>w/v</i> SA and 9% <i>w/v</i> MC was the only one showing a successful stacking and 96% recovery capacity. A 0.5 × 10<sup>6</sup> PANC-1 cell-laden bioink was extruded with an Inkredible 3D printer (Cellink) through a D = 410 μm tip conical nozzle into 6-well culture plates. Cylindrical constructs were printed and crosslinked with CaCl<sub>2</sub>. Bioinks suffered a 1.845 Pa maximum pressure at the tip that was not deleterious for cellular viability. Cell aggregates can be appreciated for the cut total length observed in confocal microscopy, indicating a good proliferation rate at different heights of the construct, and suggesting the viability of the selected bioink PANC-1/P-Alg<sub>3</sub>/MC<sub>9</sub> for building up three-dimensional bioprinted pancreatic tumor constructs.https://www.mdpi.com/2073-4360/15/15/31963D bioprintingbioinksplasmaalginatemethylcellulosePANC-1 |
| spellingShingle | Cristina Banda Sánchez Nieves Cubo Mateo Laura Saldaña Alba Valdivieso Julie Earl Itziar González Gómez Luis M. Rodríguez-Lorenzo Selection and Optimization of a Bioink Based on PANC-1- Plasma/Alginate/Methylcellulose for Pancreatic Tumour Modelling Polymers 3D bioprinting bioinks plasma alginate methylcellulose PANC-1 |
| title | Selection and Optimization of a Bioink Based on PANC-1- Plasma/Alginate/Methylcellulose for Pancreatic Tumour Modelling |
| title_full | Selection and Optimization of a Bioink Based on PANC-1- Plasma/Alginate/Methylcellulose for Pancreatic Tumour Modelling |
| title_fullStr | Selection and Optimization of a Bioink Based on PANC-1- Plasma/Alginate/Methylcellulose for Pancreatic Tumour Modelling |
| title_full_unstemmed | Selection and Optimization of a Bioink Based on PANC-1- Plasma/Alginate/Methylcellulose for Pancreatic Tumour Modelling |
| title_short | Selection and Optimization of a Bioink Based on PANC-1- Plasma/Alginate/Methylcellulose for Pancreatic Tumour Modelling |
| title_sort | selection and optimization of a bioink based on panc 1 plasma alginate methylcellulose for pancreatic tumour modelling |
| topic | 3D bioprinting bioinks plasma alginate methylcellulose PANC-1 |
| url | https://www.mdpi.com/2073-4360/15/15/3196 |
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