Fecal DNA isolation and degradation in clam Cyclina sinensis: noninvasive DNA isolation for conservation and genetic assessment
Abstract Background To avoid destructive sampling for conservation and genetic assessment, we isolated the DNA of clam Cyclina sinensis from their feces. DNA electrophoresis and PCR amplification were used to determine the quality of fecal DNA. And we analyzed the effects of different conditions on...
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| Format: | Article |
| Language: | English |
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BMC
2019-12-01
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| Series: | BMC Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12896-019-0595-6 |
| _version_ | 1818569966371209216 |
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| author | Min Zhang Min Wei Zhiguo Dong Haibao Duan Shuang Mao Senlei Feng Wenqian Li Zepeng Sun Jiawei Li Kanglu Yan Hao Liu Xueping Meng Hongxing Ge |
| author_facet | Min Zhang Min Wei Zhiguo Dong Haibao Duan Shuang Mao Senlei Feng Wenqian Li Zepeng Sun Jiawei Li Kanglu Yan Hao Liu Xueping Meng Hongxing Ge |
| author_sort | Min Zhang |
| collection | DOAJ |
| description | Abstract Background To avoid destructive sampling for conservation and genetic assessment, we isolated the DNA of clam Cyclina sinensis from their feces. DNA electrophoresis and PCR amplification were used to determine the quality of fecal DNA. And we analyzed the effects of different conditions on the degradation of feces and fecal DNA. Results The clear fecal DNA bands were detected by electrophoresis, and PCR amplification using clam fecal DNA as template was effective and reliable, suggesting that clam feces can be used as an ideal material for noninvasive DNA isolation. In addition, by analyzing the effects of different environmental temperatures and soaking times on the degradation of feces and fecal DNA, we found that the optimum temperature was 4 °C. In 15 days, the feces maintained good texture, and the quality of fecal DNA was good. At 28 °C, the feces degraded in 5 days, and the quality of fecal DNA was poor. Conclusions The clam feces can be used as an ideal material for noninvasive DNA isolation. Moreover, the quality of fecal DNA is negatively correlated with environmental temperature and soaking time. |
| first_indexed | 2024-12-14T06:54:36Z |
| format | Article |
| id | doaj.art-d872517f378d4d8393b0ba081e71b1dd |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-12-14T06:54:36Z |
| publishDate | 2019-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-d872517f378d4d8393b0ba081e71b1dd2022-12-21T23:12:45ZengBMCBMC Biotechnology1472-67502019-12-011911810.1186/s12896-019-0595-6Fecal DNA isolation and degradation in clam Cyclina sinensis: noninvasive DNA isolation for conservation and genetic assessmentMin Zhang0Min Wei1Zhiguo Dong2Haibao Duan3Shuang Mao4Senlei Feng5Wenqian Li6Zepeng Sun7Jiawei Li8Kanglu Yan9Hao Liu10Xueping Meng11Hongxing Ge12Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean UniversityJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean UniversityJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean UniversityJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean UniversityJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean UniversityJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean UniversityJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean UniversityJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean UniversityJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean UniversityJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean UniversityJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean UniversityJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean UniversityJiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean UniversityAbstract Background To avoid destructive sampling for conservation and genetic assessment, we isolated the DNA of clam Cyclina sinensis from their feces. DNA electrophoresis and PCR amplification were used to determine the quality of fecal DNA. And we analyzed the effects of different conditions on the degradation of feces and fecal DNA. Results The clear fecal DNA bands were detected by electrophoresis, and PCR amplification using clam fecal DNA as template was effective and reliable, suggesting that clam feces can be used as an ideal material for noninvasive DNA isolation. In addition, by analyzing the effects of different environmental temperatures and soaking times on the degradation of feces and fecal DNA, we found that the optimum temperature was 4 °C. In 15 days, the feces maintained good texture, and the quality of fecal DNA was good. At 28 °C, the feces degraded in 5 days, and the quality of fecal DNA was poor. Conclusions The clam feces can be used as an ideal material for noninvasive DNA isolation. Moreover, the quality of fecal DNA is negatively correlated with environmental temperature and soaking time.https://doi.org/10.1186/s12896-019-0595-6Cyclina sinensisFeces; noninvasive DNA isolationDNA degradation |
| spellingShingle | Min Zhang Min Wei Zhiguo Dong Haibao Duan Shuang Mao Senlei Feng Wenqian Li Zepeng Sun Jiawei Li Kanglu Yan Hao Liu Xueping Meng Hongxing Ge Fecal DNA isolation and degradation in clam Cyclina sinensis: noninvasive DNA isolation for conservation and genetic assessment BMC Biotechnology Cyclina sinensis Feces; noninvasive DNA isolation DNA degradation |
| title | Fecal DNA isolation and degradation in clam Cyclina sinensis: noninvasive DNA isolation for conservation and genetic assessment |
| title_full | Fecal DNA isolation and degradation in clam Cyclina sinensis: noninvasive DNA isolation for conservation and genetic assessment |
| title_fullStr | Fecal DNA isolation and degradation in clam Cyclina sinensis: noninvasive DNA isolation for conservation and genetic assessment |
| title_full_unstemmed | Fecal DNA isolation and degradation in clam Cyclina sinensis: noninvasive DNA isolation for conservation and genetic assessment |
| title_short | Fecal DNA isolation and degradation in clam Cyclina sinensis: noninvasive DNA isolation for conservation and genetic assessment |
| title_sort | fecal dna isolation and degradation in clam cyclina sinensis noninvasive dna isolation for conservation and genetic assessment |
| topic | Cyclina sinensis Feces; noninvasive DNA isolation DNA degradation |
| url | https://doi.org/10.1186/s12896-019-0595-6 |
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